Glioma growth inhibition by neurostatin and O-But GD1b.

Neural Plasticity Group, Functional and Systems Neurobiology Department, Instituto Cajal, Madrid, Spain.
Neuro-Oncology (Impact Factor: 5.56). 11/2010; 12(11):1135-46. DOI: 10.1093/neuonc/noq073
Source: PubMed


In spite of their low incidence, central nervous system tumors have elevated morbidity and mortality, being responsible for
2.3% of total cancer deaths. The ganglioside O-acetylated GD1b (O-Ac GD1b; neurostatin), present in the mammalian brain, and
the semi-synthetic O-butyrylated GD1b (O-But GD1b) are potent glioma proliferation inhibitors, appearing as possible candidates
for the treatment of nervous system tumors. Tumoral cell division inhibitory activity in culture correlated with growth inhibition
of glioma xenotransplants in Foxn1nu nude mice and intracranial glioma allotransplants. Both O-Ac GD1b and O-But GD1b inhibited in vivo cell proliferation, induced
cell cycle arrest, and potentiated immune cell response to the tumor. Furthermore, the increased stability of the butyrylated
compound (O-But GD1b) enhanced its activity with respect to the acetylated ganglioside (neurostatin). These results are the
first report of the antitumoral activity of neurostatin and a neurostatin-like compound in vivo and indicate that semi-synthetic
O-acetylated and O-butyrylated gangliosides are potent antitumoral compounds that should be considered in strategies for brain
tumor treatment.

Download full-text


Available from: Manuel Nieto-Sampedro
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The high frequency and malignancy of human glioblastomas has stimulated the search for potential therapeutic approaches. The control of the glioma cell proliferation in response to mitogenic signals is one of the most promising antitumoral strategies, and the main target of several therapies. Neurostatin, an O-acetylated derivative of the ganglioside GD1b, has potent antiproliferative activity over the in vitro and in vivo growth of glioma cells. The mechanism of its antitumoral action is the focus of the present study. Using a combined in vitro-in vivo approach, we observed that neurostatin arrested glioma proliferation by inhibiting the expression of cell cycle promoters (i.e. cyclins and CDKs) and promoting the expression of cell cycle inhibitors (i.e. p21 and p27). Neurostatin inhibits epidermal growth factor receptor (EGFR) signaling pathways, blocking the activation of the main promitogenic MAPKs and PI3K pathways. Neurostatin action not only interferes in the cell cycle progression, but also in the protection from apoptosis, and the generation of angiogenic and invasive responses. The antitumoral actions described here point to neurostatin as a novel and promising chemotherapeutic agent for glioma treatment.
    Full-text · Article · Jan 2011 · Molecular and Cellular Neuroscience
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The impact of inflammation is crucial for the regulation of the biology of neural stem cells (NSCs). Interleukin-15 (IL-15) appears as a likely candidate for regulating neurogenesis, based on its well-known mitogenic properties. We show here that NSCs of the subventricular zone (SVZ) express IL-15, which regulates NSC proliferation, as evidenced by the study of IL-15-/- mice and the effects of acute IL-15 administration, coupled to 5-bromo-2'-deoxyuridine/5-ethynyl-2'-deoxyuridine dual-pulse labeling. Moreover, IL-15 regulates NSC differentiation, its deficiency leading to an impaired generation of neuroblasts in the SVZ-rostral migratory stream axis, recoverable through the action of exogenous IL-15. IL-15 expressed in cultured NSCs is linked to self-renewal, proliferation, and differentiation. IL-15-/- NSCs presented deficient proliferation and self-renewal, as evidenced in proliferation and colony-forming assays and the analysis of cell cycle-regulatory proteins. Moreover, IL-15-deficient NSCs were more prone to differentiate than wild-type NSCs, not affecting the cell population balance. Lack of IL-15 led to a defective activation of the JAK/STAT and ERK pathways, key for the regulation of proliferation and differentiation of NSCs. The results show that IL-15 is a key regulator of neurogenesis in the adult and is essential to understanding diseases with an inflammatory component.
    Full-text · Article · Jun 2011 · Molecular biology of the cell