Distinctive Pattern of Glial Fibrillary Acidic Protein Immunoreactivity Useful in Distinguishing Fragmented Pleomorphic Adenoma, Canalicular Adenoma and Polymorphous Low Grade Adenocarcinoma of Minor Salivary Glands

Department of Diagnostic Sciences, Division of Oral and Maxillofacial Pathology, University of North Carolina School of Dentistry, Chapel Hill, NC 27599, USA.
Head and Neck Pathology 09/2007; 1(1):27-32. DOI: 10.1007/s12105-007-0003-8
Source: PubMed


Immunohistochemistry (IHC) can be helpful in the diagnosis of minor salivary gland neoplasms including those that have been incisionally biopsied or fragmented during surgery that do not contain key diagnostic features on hematoxylin and eosin sections. IHC has been used as an adjunct to distinguish among many salivary gland neoplasms using both qualitative and quantitative methods. The objective of this study was to determine whether a distinctive immunoreactivity staining pattern to GFAP can be consistently observed among three selected minor salivary gland neoplasms and thus serve as a diagnostic adjunctive procedure.
Glial fibrillary acidic protein (GFAP) reactivity was examined among 78 minor salivary gland neoplasms: 27 canalicular adenomas (CAA), 21 pleomorphic adenomas (PA) and 30 polymorphous low grade adenocarcinomas (PLGA). Each case was evaluated by two oral and maxillofacial pathologists (OMP) blinded to the diagnosis. Consensus was reached on the pattern of GFAP reactivity among the neoplastic cells and on the similarities and differences among the cases.
Ninety-six percent (96%) of CAAs demonstrated a distinctive linear immunoreactive pattern among cells in proximity to connective tissue interface. All (100%) PAs demonstrated diffuse immunopositivity within tumor cells. All (100%) PLGAs showed little or no intralesional reactivity and no peripheral linear immunoreactivity. Additional challenge cases were examined by outside OMPs to demonstrate the utility of these findings.
This study demonstrates that the pattern of GFAP immunoreactivity may be an adjunct to diagnosis among PA, CAA and PLGA. The pattern of distinctly linear GFAP immunoreactivity at the tumor/connective tissue interface in CAA has not been reported previously. This distinctive feature may permit the pathologist to differentiate among CAA, PA and PLGA when an incisional biopsy and/or fragmentation cause key diagnostic features to be absent. Because each of these neoplasms requires a different treatment approach, this can be of major significance.

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Available from: Valerie A Murrah, Jun 17, 2015
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    • "Multiple studies have been performed to investigate the role of immunomarkers in salivary gland tumors. A large number of studies have evaluated the role of CD 117 (c-kit) and smooth muscle actin (SMA) in differentiating AdCC from PLGA [13–17], while glial fibrillary acidic protein (GFAP) seems promising in differentiating between PA and PLGA [12, 18–20]. In salivary gland pathology, lot many antibodies have been applied to analyse different tumor cell types in order to establish differences among tumor types and highly variable results have been reported. "
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