Blood Transfus 2010; 8 Suppl 3:s113-s119 DOI 10.2450/2010.018S
© SIMTI Servizi Srl
Adsorption and carbonylation of plasma proteins by dialyser membrane
material: in vitro and in vivo proteomics investigations
Barbara Pavone1,2,4, Vittorio Sirolli3, Sonia Bucci1,2, Fulvio Libardi3, Paolo Felaco3,
Luigi Amoroso3, Paolo Sacchetta2, Andrea Urbani4,5,, Mario Bonomini3
1Centre of Investigation on Aging (Ce.S.I.), "G. d'Annunzio" University Foundation, Chieti-Pescara; 2Department
of Biomedical Sciences "G. d'Annunzio" University , Chieti-Pescara; 3 Institute of Nephrology , Department of
Medicine, "G. d'Annunzio" University, Chieti-Pescara; 4IRCCS-Santa Lucia Foundation, Rome; 5Department
of Internal Medicine, University "Tor Vergata", Rome, Italy.
Background. Protein carbonylation is an irreversible and not reparable reaction which is
caused by the introduction into proteins of carbonyl derivatives such as ketones and aldehydes,
generated from direct oxidation processes or from secondary protein reaction with reactive
carbonyl compounds. Several studies have demonstrated significantly increased levels of reactive
carbonyl compounds, a general increase in plasma protein carbonyls and carbonyl formation on
major plasma proteins in blood from uremic patients, particularly those undergoing chronic
Materials and methods. In the present preliminary study, we first assessed by an in vitro
filtration apparatus the possible effects of different materials used for haemodialysis membranes
on protein retention and carbonylation. We employed hollow fiber minidialyzers of identical
structural characteristics composed of either polymethylmethacrylate, ethylenevinyl alcohol, or
cellulose diacetate materials. Protein Western Blot and SDS-PAGE coupled to mass spectrometry
analysis were applied to highlight the carbonylated protein-binding characteristics of the different
materials. We also investigated in vivo protein carbonylation and carboxy methyl lisine-
modification in plasma obtained before and after a haemodialysis session.
Results. Our data underline a different capability on protein adsorption associated with the
different properties of the filter materials, highlighting the central buffering and protective role
of serum albumin. In particular, polymethylmethacrylate and cellulose diacetate showed, in vitro,
the highest capacity of binding plasma proteins on the surface of the hollow fiber minidialyzers.
Conclusions. The present study suggests that biomaterials used for fabrication of
haemodialysis membrane may affect the carbonyl balance in chronic uremic patients.
Key words: minidialyzer, haemodialysis membrane, carbonylated proteins, carboxy methyl
Protein carbonylation is an irreversible and not
reparable reaction which is caused by the introduction
into proteins of carbonyl derivatives such as ketones
and aldehydes, generated from direct oxidation
processes or from secondary protein reaction with
reactive carbonyl compounds, eventually forming
advanced glycation end products (AGEs) and
advanced lipoxidation end products (ALEs)1. Loss of
protein function usually stem from the carbonylation
process and may lead to cellular dysfunction and tissue
damage2. Carbonyl stress-modified proteins can also
and haemodialysis such as dialysis-related
amyloidosis and accelerated atherosclerosis1,20. Our
study suggests that haemodialytic membranes may
affect the carbonyl balance in chronic uremic patients,
though the study design does not allow us to draw
any definitive conclusion on the issue.
At present, however, these assumptions are only
speculative and require further investigation in vivo
with the aim to verify that adsorption onto the
haemodialysis membrane surface might represent a
mechanism to antagonize protein carbonylation in
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Correspondence : Mario Bonomini
Clinica Nefrologica - Emodialisi
Ospedale Clinicizzato “SS. Annunziata”
Via dei Vestini
66013 Chieti, Italy
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Hemodialysis membrane and protein carbonylation
Blood Transfus 2010; 8 Suppl 3:s113-s119 DOI 10.2450/2010.018S