Potential of the TCE-Degrading Endophyte Pseudomonas putida W619-TCE to Improve Plant Growth and Reduce TCE Phytotoxicity and Evapotranspiration in Poplar Cuttings

Hasselt University, Centre for Environmental Sciences, Agoralaan Building D, B-3590 Diepenbeek, Belgium.
Environmental Pollution (Impact Factor: 4.14). 09/2010; 158(9):2915-9. DOI: 10.1016/j.envpol.2010.06.004
Source: PubMed


The TCE-degrading poplar endophyte Pseudomonas putida W619-TCE was inoculated in poplar cuttings, exposed to 0, 200 and 400 mg l(-1) TCE, that were grown in two different experimental setups. During a short-term experiment, plants were grown hydroponically in half strength Hoagland nutrient solution and exposed to TCE for 3 days. Inoculation with P. putida W619-TCE promoted plant growth, reduced TCE phytotoxicity and reduced the amount of TCE present in the leaves. During a mid-term experiment, plants were grown in potting soil and exposed to TCE for 3 weeks. Here, inoculation with P. putida W619-TCE had a less pronounced positive effect on plant growth and TCE phytotoxicity, but resulted in strongly reduced amounts of TCE in leaves and roots of plants exposed to 400 mg l(-1) TCE, accompanied by a lowered evapotranspiration of TCE. Dichloroacetic acid (DCAA) and trichloroacetic acid (TCAA), which are known intermediates of TCE degradation, were not detected.

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    • "Genes involved in the catabolism of a wide variety of organic compounds, including many naturally-occurring products (eg, metabolites from the partial degradation of lignin), are chromosomally encoded in the P. putida genome. As stated above, some strains of this species colonize the rhizosphere of plants (Molina et al., 2000; Weyens et al., 2010; Li et al., 2013; Roca et al., 2013). This characteristic, "
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    ABSTRACT: Pseudomonas putida are strict aerobes that proliferate in a range of temperate niches and are of interest for environmental applications due to their capacity to degrade pollutants and ability to promote plant growth. Furthermore solvent tolerant strains are useful for biosynthesis of added-value chemicals. We present a comprehensive comparative analysis of nine strains and the first characterization of the Pseudomonas putida pangenome. The core genome of P. putida comprises approximately 3386 genes. The most abundant genes within the core genome are those that encode nutrient transporters. Other conserved genes include those for central carbon metabolism through the Entner-Doudoroff pathway, the pentose phosphate cycle, arginine and proline metabolism, and pathways for degradation of aromatic chemicals. Genes that encode transporters, enzymes and regulators for amino acid metabolism (synthesis and degradation) are all part of the core genome, as well as various electron transporters, which enable aerobic metabolism under different oxygen regimes. Within the core genome are thirty genes for flagella biosynthesis and twelve key genes for biofilm formation. Pseudomonas putida strains share 85% of the coding regions with Pseudomonas aeruginosa; however, in P. putida, virulence factors such as exotoxins and type III secretion systems are absent. This article is protected by copyright. All rights reserved.
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    • "Recently, it was proposed that the addition of plant growthpromoting bacteria (preferably endophytes) is a simple and effective strategy to enhance plant growth and remediation of contaminants from the soil [9] [10]. Endophytic bacteria can assist their host plant to overcome pollutant-induced stress responses and consequently improve plant growth and phytoremediation activity [11] [12]. Several endophytic bacteria can also survive and colonize in the soil surrounding the roots, where they can penetrate into their associated plant via the roots [13], however, it was also observed that some phyllosphere bacteria may be a source of endophytic bacteria [14] [15]. "
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    ABSTRACT: The aim of this study was to determine whether the inoculation of plant growth-promoting bacteria to plants, vegetated in soil irrigated with textile effluent, influences plant biomass production, and soil remediation. Three different plant species (Acacia ampliceps, Eucalyptus camaldulensis, and Leucaena leucocephala) with and without bacterial inoculation were grown in soil irrigated with secondary treated textile wastewater for one year. An endophytic bacterium, Burkholderia sp. strain PsJN, possessing plant growth-promoting 1-aminocyclopropane-1-carboxylate deaminase activity was inoculated to plants. There was more plant biomass production (up to 12%) and contaminants removal (up to 29%) from soil with bacterial inoculation as compared to soil having non-inoculated plants. Enhanced plant growth and soil remediation activity are associated with the survival and colonization of the inoculated bacterium in the rhizosphere and endosphere of plants. The highest plant biomass production and contaminants removal from soil were observed in the treatment, in which A. ampliceps was inoculated with Burkholderia sp. strain PsJN. These results suggest that plant-bacteria partnerships can be applied to improve plant growth and soil remediation during the application of industrial effluent for plant biomass production in the arid regions.
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    • "Inoculated bacteria may transfer pollutant-degrading genes to the indigenous endophytic bacterial community through horizontal gene transfer as shown by Weyens et al. (2010b). Horizontal gene transfer plays a significant role in the adaptation of microbial communities to environmental stress (van der Lelie et al., 2005). "
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    ABSTRACT: Recently, there has been an increased effort to enhance the efficacy of phytoremediation of contaminated environments by exploiting plant-microbe interactions. The combined use of plants and endophytic bacteria is an emerging approach for the clean-up of soil and water polluted with organic compounds. In plant-endophyte partnerships, plants provide the habitat as well as nutrients to their associated endophytic bacteria. In response, endophytic bacteria with appropriate degradation pathways and metabolic activities enhance degradation of organic pollutants, and diminish phytotoxicity and evapotranspiration of organic pollutants. Moreover, endophytic bacteria possessing plant growth-promoting activities enhance the plant's adaptation and growth in soil and water contaminated with organic pollutants. Overall, the application of endophytic bacteria gives new insights into novel protocols to improve phytoremediation efficiency. However, successful application of plant-endophyte partnerships for the clean-up of an environment contaminated with organic compounds depends on the abundance and activity of the degrading endophyte in different plant compartments. Although many endophytic bacteria have the potential to degrade organic pollutants and improve plant growth, their contribution to enhance phytoremediation efficiency is still underestimated. A better knowledge of plant-endophyte interactions could be utilized to increase the remediation of polluted soil environments and to protect the foodstuff by decreasing agrochemical residues in food crops.
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