Determination of organic acids in Vaccinium berry standard reference materials

ArticleinAnalytical and Bioanalytical Chemistry 398(1):425-34 · September 2010with130 Reads
DOI: 10.1007/s00216-010-3916-0 · Source: PubMed
Abstract
Nine organic acids (citric acid, galacturonic acid, glycolic acid, isocitric acid, malic acid, oxalic acid, quinic acid, shikimic acid, and tartaric acid) and two anions (phosphate and sulfate) were determined in a suite of Vaccinium berry-containing dietary supplement standard reference materials (SRMs). Following solvent extraction, three independent methods were utilized in the quantification of these compounds. The first method involved reversed-phase liquid chromatography with ultraviolet absorbance detection at 210 nm and isotope dilution mass spectrometry. The second method utilized ion chromatography with conductivity detection. Finally, gas chromatography with isotope dilution mass spectrometry detection was used following derivatization with N-methyl-N-trifluoroacetamide (MSTFA). The combined data from these methods was used for the assignment of organic acid levels in the seven candidate SRMs.
    • "The extraction procedure was repeated two times more for each cultivar. For the organic acids determinations, the method suggested by Phillips et al. [26] was used. One gram of DM was homogenized into 10 mL of bidistilled water using a commercial sonicator (model Sonorex RK 100, Bandelin, Germany). "
    [Show abstract] [Hide abstract] ABSTRACT: In recent years the consumption of small fruits has increased continuously and knowledge about the more suitable production requirements is essential. This study aims to evaluate the influence of harvest year and altitude on chemical composition of four blueberry cultivars, in order to create rentable opportunities for producers and minimize eventual losses in quality fruits. Dry mass, protein, fat, energy, free sugars, organic acids, and vitamin C contents were determined using HPLC-UV-DAD and spectrophotometric methods. Differences ( p < 0.05 ) in the concentrations of free sugars, organic acids, and vitamin C were found among years, while the altitude had no ( p > 0.05 ) influence. Citric acid was the main organic acid and fructose the most abundant sugar in blueberries. Fruits of “Ozarkblue” had the highest mass and volume whilst the “Bluecrop” was the cultivar with highest crude protein and fat contents. “Goldtraube” showed the highest content of sucrose and organic acids and “Duke” had the highest content of fructose.
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    • "Juices were centrifuged for 20 min at 4600Âg (IEC CL) and filtered through 0.45 lm polyvinlydiflouride (PVDF) syringe filters (Restek). Filtrate was placed in 10 mL autosampler vials, sealed with Teflon lined silicon septa (Thermo Fisher Scientific, Waltham, MA) and analyzed on a Dionex HPLC (Sunnyvale, CA) with an AS50 autosampler, a GP50 gradient pump, a LC30 chromatography oven at 30 °C and a PDA100 photo diode array detector collecting absorbance data at 215 nm, controlled by Chromeleon software (Thermo Fisher Scientific, Waltham, MA), using a modified method (Phillips et al., 2010). Twenty microliter juice was injected into an Allure Organic Acids 300 mm HPLC column , also protected by a guard column (Restek). "
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    • "Other newly bred genotypes , 'Danute ˙ ' and 'Freda' also contained more than 3 mg/mL, as well as known cultivar 'Patriot'. Previously, an average concentration of quinic acid in solvent extract of blueberry freeze-dried powdered material was reported 25.5 mg/g (Phillips et al., 2010). However, to the best of our knowledge there is no published data about quinic acid concentration in V. corymbosum berry juices. "
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