Rapid Detection of Trypanosoma cruzi in Human Serum by Use of an Immunochromatographic Dipstick Test

Department of Infectious & Tropical Diseases, London School of Hygiene & Tropical Medicine, London, United Kingdom.
Journal of clinical microbiology (Impact Factor: 3.99). 08/2010; 48(8):3003-7. DOI: 10.1128/JCM.02474-09
Source: PubMed


We evaluated a commercially available immunochromatographic dipstick test to detect Trypanosoma cruzi infection in 366 human serum samples with known serological results from Argentina, Ecuador, Mexico, and Venezuela. One hundred forty-nine of 366 (40.7%) and 171/366 (46.7%) samples tested positive by dipstick and serology, respectively. Dipstick sensitivity was calculated to be 84.8% (range between countries, 77.5 to 95%), and specificity was 97.9% (95.9 to 100%). Copyright © 2010, American Society for Microbiology. All Rights Reserved.

Download full-text


Available from: Jaime R Torres
  • Source
    • "seropositive sera (Padilla et al. 2002) and by evaluating three different T. cruzi tests on samples from each individual dog. We evaluated how likely were the results similar by mere chance through the estimation of Cohen's coefficient of agreement Kappa (Cohen, 1960), between the Leishmania IFAT and ELISA diagnostic tests with a rapid immunochromatographic test for Chagas disease (Reithinger et al. 2010), a T. cruzi ELISA and a T. cruzi IFAT (Pineda et al. 2011), and a gold standard based on seropositive reactions for T. cruzi by at least two different tests, following WHO recommendation for T. cruzi serological diagnosis in humans (Pineda et al. 2011). "
    [Show abstract] [Hide abstract]
    ABSTRACT: American cutaneous leishmaniasis (ACL) is a complex disease with a rich diversity of animal host species. This diversity imposes a challenge, since understanding ACL transmission requires the adequate identification of reservoir hosts, those species able to be a source of additional infections. In this study we present results from an ACL cross-sectional serological survey of 51 dogs ( Canis familiaris ), where we used diagnostic tests that measure dog's exposure to Leishmania spp. parasites. We did our research in Panamá, at a village that has undergone significant ecosystem level transformations. We found an ACL seroprevalence of 47% among dogs, and their exposure was positively associated with dog age and abundance of sand fly vectors in the houses of dog owners. Using mathematical models, which were fitted to data on the proportion of positive tests as function of dog age, we estimated a basic reproductive number ( R 0 ± s.e. ) of 1·22 ± 0·09 that indicates the disease is endemically established in the dogs. Nevertheless, this information by itself is insufficient to incriminate dogs as ACL reservoirs, given the inability to find parasites (or their DNA) in seropositive dogs and previously reported failures to experimentally infect vectors feeding on dogs with ACL parasites.
    Full-text · Article · May 2015 · Parasitology
  • Source
    • "Several studies have evaluated the performance of these tests with serum samples in the laboratory (Ponce et al. 2005, Reithinger et al. 2010). In a recent multicentre "
    [Show abstract] [Hide abstract]
    ABSTRACT: Many patients with Chagas disease live in remote communities that lack both equipment and trained personnel to perform a diagnosis by conventional serology (CS). Thus, reliable tests suitable for use under difficult conditions are required. In this study, we evaluated the ability of personnel with and without laboratory skills to perform immunochromatographic (IC) tests to detect Chagas disease at a primary health care centre (PHCC). We examined whole blood samples from 241 patients and serum samples from 238 patients. Then, we calculated the percentage of overall agreement (POA) between the two groups of operators for the sensitivity (S), specificity (Sp) and positive (PPV) and negative (NPV) predictive values of IC tests compared to CS tests. We also evaluated the level of agreement between ELISAs and indirect haemagglutination (IHA) tests. The readings of the IC test results showed 100% agreement (POA = 1). The IC test on whole blood showed the following values: S = 87.3%; Sp = 98.8%; PPV = 96.9% and NPV = 95.9%. Additionally, the IC test on serum displayed the following results: S = 95.7%; Sp = 100%; PPV = 100% and NPV = 98.2%. Using whole blood, the agreement with ELISA was 96.3% and the agreement with IHA was 94.1%. Using serum, the agreement with ELISA was 97.8% and the agreement with IHA was 96.6%. The IC test performance with serum samples was excellent and demonstrated its usefulness in a PHCC with minimal equipment. If the IC test S value and NPV with whole blood are improved, then this test could also be used in areas lacking laboratories or specialised personnel.
    Full-text · Article · Dec 2014 · Memórias do Instituto Oswaldo Cruz
  • Source

    Full-text · Article · Oct 2011 · PLoS Neglected Tropical Diseases
Show more