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Evaluation of the Polyphenol Content and Antioxidant Properties of Methanol Extracts of the Leaves, Stem And Root Barks Of

Biochemistry Department, Ahmadu Bello University, Zaria, Nigeria.
Journal of medicinal food (Impact Factor: 1.63). 06/2010; 13(3):710-6. DOI: 10.1089/jmf.2009.0057
Source: PubMed

ABSTRACT

Medicinal plants have been shown to have both chemopreventive and/or therapeutic effects on cancer and other diseases related to oxidative damage. Moringa oleifera Lam., known in the Hausa and Igala languages of Nigeria as "Zogale" and "Gergedi," respectively, and drumstick in English, is a plant that is used both as food and in folkloric medicine in Nigeria and elsewhere. Different parts of the plant were analyzed for polyphenol content as well as in vitro antioxidant potential. The methanol extract of the leaves of M. oleifera contained chlorogenic acid, rutin, quercetin glucoside, and kaempferol rhamnoglucoside, whereas in the root and stem barks, several procyanidin peaks were detected. With the xanthine oxidase model system, all the extracts exhibited strong in vitro antioxidant activity, with 50% inhibitory concentration (IC(50)) values of 16, 30, and 38 microL for the roots, leaves, and stem bark, respectively. Similarly, potent radical scavenging capacity was observed when extracts were evaluated with the 2-deoxyguanosine assay model system, with IC(50) values of 40, 58, and 72 microL for methanol extracts of the leaves, stem, and root barks, respectively. The high antioxidant/radical scavenging effects observed for different parts of M. oleifera appear to provide justification for their widespread therapeutic use in traditional medicine in different continents. The possibility that this high antioxidant/radical scavenging capacity may impact on the cancer chemopreventive potential of the plant must be considered.

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    • "Kumari[40]also examined the hypoglycemic effect of M. oleifera leaf dietary consumption in T2DM patients, and reported that it reduced glucose level significantly after a 40-day period. The therapeutic actions of Moringa oleifera medication has been attributed to the relatively high antioxidant activity of its leaves, flowers, and seeds[41,42,43,44]. Moringa oleifera leaves and seeds contain many powerful antioxidant phytochemicals, especially quercetin and kaempferol[33]. "

    Full-text · Article · Jan 2016
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    • "Kumari (2010) also reported significant hypoglycaemic effect of M. oleifera leaf dietary consumption in Type 2 diabetes mellitus (T2DM) patients, and reported that it reduced glucose level significantly after a 40-day period. The therapeutic actions of Moringa oleifera medication have been attributed to the relatively high antioxidant activity of its leaves, flowers, and seeds (Atawodi et al., 2010). Quercetin and kaemferol found in Moringa oleifera leaves were shown to be antioxidant in nature (Fuglie, 1999). "

    Full-text · Article · Dec 2015
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    • "This was fairly low but significantly different from that of the reference standard (2.10 µg/mL). The observed result was similar to the findings of Atawodi et al. (2010). The DPPH radical is one of the few stable organic nitrogen radicals, with a deep purple colour. "
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    ABSTRACT: Moringa oleifera, a plant with a wide range of medicinal, nutritional and economic benefits was examined for phytochemicals and evaluated for antioxidant activities. Phytochemical tests, total phenol and flavonoid contents were determined using standard procedures. Antioxidant activities of the methanol extracts and fractions were evaluated using the 2,2-diphenyl-1-picryl hydrazyl radical scavenging assay. The leaf and seed contained alkaloids, saponins, phenols and flavonoids. The ethyl acetate fraction of the leaf contained significantly (P < 0.05) more phenol (78.67 ± 3.40 mgGAE/g Extract) than the crude extract and petroleum ether fraction with total phenolic content of 58.00 ± 1.00 and 26.17 ± 3.88 mg GAE/g Extract respectively while the seed extract had a total phenol content of 17.67 ± 2.02 mg GAE/g Extract. The petroleum ether fraction of the leaf was found to contain significantly (P < 0.05) more flavonoid (161.33 ± 19.05 mgQE/g Extract) than the other samples (31.73 ± 2.66, 30.20 ± 10.14 and 20.73 ± 4.16 mgQE/g Extract for the crude leaf extract, leaf petroleum ether fraction and seed extract respectively). The leaf and seed extracts exhibited remarkable and concentration-dependent increase in radical scavenging activities with IC50 values ranging from 5.72-42.56 μg/mL. The results therefore support the use of Moringa oleifera as a natural plant antioxidant by preventing free radical damage.
    Full-text · Article · Jan 2015
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