Biophysics: Transporter in the spotlight

Department of Cell Biology, NYU Langone Medical Center, New York, New York, United States
Nature (Impact Factor: 41.46). 05/2010; 465(7295):171-2. DOI: 10.1038/465171a
Source: PubMed


Membrane transporter proteins switch between conformational states to
move substrates across membranes. The transition between these states
can now be studied using single-molecule experiments.

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Available from: Da-Neng Wang
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    ABSTRACT: OxlT, the oxalate transporter of Oxalobacter formigenes, is a member of the Major Facilitator Superfamily of transporters (MFS), one of the largest groups of membrane proteins with substantial relevance to solute transport physiology, pharmacology, and possible drug development. MFS proteins transport a wide range of substrates such as organic and inorganic anions, sugars, drugs, and neurotransmitters. This review succinctly summarizes experimental work on a model MFS protein, OxlT, beginning with its identification as an electrogenic oxalate/formate exchanger, its three-dimensional structure, and discussion of biochemical and biophysical data that have shed further light on its structure and function. We also discuss the structure and function of OxlT in relation to notable MFS carriers such as LacY and GlpT.
    No preview · Article · Sep 2014 · Journal of Membrane Biology