Constraint of DNA on Functionalized Graphene Improves its Biostability and Specificity

Pacific Northwest National Laboratory, Richland, WA 99352, USA.
Small (Impact Factor: 8.37). 06/2010; 6(11):1205-9. DOI: 10.1002/smll.201000024
Source: PubMed


The single-stranded DNA constrained on graphene surface is effectively protected from enzymatic cleavage by DNase I. The anisotropy, fluorescence, NMR, and CD studies suggest that the single-stranded DNA is promptly adsorbed onto graphene forming strong molecular interactions. Furthermore, the constraint of DNA probe on graphene improves the specificity of its response to complementary DNA. These findings will promote the further application of graphene in biotechnology and biomedical fields.

Download full-text


Available from: Garry W Buchko
  • Source
    • "In particular, we functionalized GIANs with Sgc8 aptamers, which selectively bind to protein tyrosine kinase 7 (PTK7) cell membrane proteins, to investigate the targeted imaging capability of GIANs49. Single-stranded nucleic acids are known to adsorb on a graphene surface through strong π-π interactions, thereby providing a simple and efficient way to functionalize graphitic nanomaterials505152. Fig. 4a schematically illustrates Sgc8 aptamer-functionalized GIANs. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Using nanomaterials to develop multimodal systems has generated cutting-edge biomedical functions. Herein, we develop a simple chemical-vapor-deposition method to fabricate graphene-isolated-Au-nanocrystal (GIAN) nanostructures. A thin layer of graphene is precisely deposited on the surfaces of gold nanocrystals to enable unique capabilities. First, as surface-enhanced-Raman-scattering substrates, GIANs quench background fluorescence and reduce photocarbonization or photobleaching of analytes. Second, GIANs can be used for multimodal cell imaging by both Raman scattering and near-infrared (NIR) two-photon luminescence. Third, GIANs provide a platform for loading anticancer drugs such as doxorubicin (DOX) for therapy. Finally, their NIR absorption properties give GIANs photothermal therapeutic capability in combination with chemotherapy. Controlled release of DOX molecules from GIANs is achieved through NIR heating, significantly reducing the possibility of side effects in chemotherapy. The GIANs have high surface areas and stable thin shells, as well as unique optical and photothermal properties, making them promising nanostructures for biomedical applications.
    Full-text · Article · Sep 2014 · Scientific Reports
  • Source
    • "In the other words, without any chemical or biochemical functionalization, graphene is difficult to show the reliable BRE characteristics. In fact, there have been several attempts to functionalize graphene with biomolecules by processing with some chemicals [20] [21]. Thus, graphene can be a better candidate material for SPR sensors due to its excellent electrophotonic property with high SVR and tunable biocompatibility by surface functional- ization. "
    [Show abstract] [Hide abstract]
    ABSTRACT: In this study, a surface plasmon resonance (SPR) based fiber optic sensor coated with graphene is introduced. A graphene film synthesized by thermal chemical vapor deposition (TCVD) is transferred onto the sensing area of the optical fiber. The detection mechanism of this sensor is based on the principle that the SPR signal changes according to the refractive indices of analytes. Biotinylated Double Crossover DNA (DXB) lattice and protein Streptavidin (SA) were used for the evaluation. To the best of our knowledge, this is the first attempt to use graphene as a replacement for conventional metal films. The exact SPR phenomena and the red-shift of 7.276 nm for the DXB and SA combination were observed.
    Full-text · Article · Oct 2013 · Sensors and Actuators B Chemical
  • Source
    • "With an atomically thick sheet structure and high conductivity , graphene has attracted intense interest in the field of biomedicine and biotechnology [1] [2] [3] [4] [5] [6] [7]. Recently great attention has been focused on the preparation of graphene oxide (GO) and reduced graphene oxide (RGO) [8] [9] [10], which provides the potential of cost-effective, large-scale production of graphene-based materials [11]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Few-layer graphene prepared from graphite exfoliated by chitosan has a preserved structure without oxidation or destruction of the sp(2) character of the carbon plane and exhibits a higher peroxidase catalytic activity than that of graphene oxide (GO) and its reduced form. The peroxidase catalytic activity of as-obtained few-layer graphene is 45 times higher than that of GO and 4 times higher than that of reduced GO with the same concentration of 30 mu g mL(-1) and the detection limit of hydrogen peroxide is 10 nM. The excellently catalytic performance can be attributed to the fast electron transfer on the surface of few-layer graphene, which is further confirmed by electrochemical characterization. The as-prepared few-layer graphene has been used to determine hydrogen peroxide in three real water samples with satisfactory results. (c) 2013 Elsevier Ltd. All rights reserved.
    Full-text · Article · Oct 2013 · Carbon
Show more