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Manual de Laboratorio de la OMS Para el Examen del Semen Humano y de la interacciön Entre el Semen y el Moco Cervical

SERBIULA (sistema Librum 2.0) 01/1987;
Source: OAI

ABSTRACT

Traducción de: Who laboratory manual for the examination of human semen and sperm-cervical mucus interaction

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    ABSTRACT: RESUMEN Antecedentes: la infertilidad puede alterar la armonía psicológica de una pareja, su vida sexual y función social. Objetivo: conocer las características generales de pacientes con infertilidad (sociales, clínicas, diagnósticas y terapéuticas) que acuden al Departamento de Biología de la Reproducción Humana del Hospital Juárez de México. Pacientes y métodos: se realizó un estudio descriptivo, observacional, indagatorio situacional y retrospectivo que incluyó 116 pacientes con infertilidad, atendidas en la consulta externa del Departamento de Biología de la Reproducción Humana del Hospital Juárez de México, del 1 de enero al 31 de diciembre de 1999. Todas ingresaron al protocolo de infertilidad y tratamiento seleccionado. Resultados: el factor endocrino-ovárico alterado fue el más frecuente (82.7%), seguido por el factor cervical (80%), el factor masculino (38%) y el factor tuboperitoneal (29%). En la mayoría de los pacientes la causa fue multifactorial. El porcentaje de embarazos logrados (31.8%) fue similar al informado en la bibliografía Conclusiones: la frecuencia de factores que influyen en la infertilidad fue similar a la observada en clínicas especializadas de países prósperos, lo mismo que en tasas de embarazo. Palabras clave: infertilidad, epidemiología. ABSTRACT Background: Infertility is defined as the failure to conceive after a year of sexual life without a method of birth control. Most studies indicate that 15% of all couples will experience primary or secondary infertility in some moment of their reproductive life. Objective: To gain knowledge of general characteristics from patients with infertility in our environment (social, clinical, diagnostic and therapeutic) that attended the Reproductive Biology Department of Hospital Juárez de Mexico. Patients and methods: A descriptive, observational, situational, and retrospective indagatory study was performed on 116 infertile patients, seen in the outpatient clinic, from January through December 1999. All had entered our protocol of infertility and selected treatment. Results: The altered ovarian endocrine factor was the most frequent (82.7%), followed by cervical factor (80%), masculine factor (38%) and tuboperitoneal factor (29%). In most cases the cause was multifactorial. The percentage of successful pregnancies (31.88%) was similar to that reported in the literature. Conclusions: The most common factors that influence infertility resembled those exhibited by specialized clinics of affluent countries with similar pregnancy rates.
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    ABSTRACT: The study of interspecific gamete interactions has been improved by the use of in vitro fertilization techniques. Zona pellucida is at the cellular level the main barrier to cross-fertilization. Moreover, there are several cases in which the heterologous spermatozoa fail to enter the cytoplasm of zona-free oocytes, emphasizing the notion that another important barrier exists to cross-fertilization at the egg plasma membrane level. We report here a simple method to fertilize hamster oocytes with zona pellucida by human spermatozoa. We used lysophosphatidylcholine (LPC), a fusogenic agent, to treat spermatozoa, spermatozoa and zona-intact oocytes, or zona-intact oocytes alone. This molecule allows the penetration of hamster oocytes zona by human spermatozoa. Our results suggest that LPC modifies the zona pellucida of oocytes and the plasma membrane of both gametes in vitro, favouring sperm penetration through the zona pellucida and gamete fusion.
    No preview · Article · Feb 1995 · Journal of Experimental Zoology
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    ABSTRACT: Objective: analysing abnormalities in seminal parameters and their correlation with age through spermograms of patients admitted to the GESTAMOS Reproductive Medicine Unit for infertility. Methods and materials: this was a descriptive cross-sectional study of 226 spermogramscollected between January 2005 and January 2008. They were examined by standard analysis according to WHO guidelines and CECOLFES Fertility and Sterility Centre's Andrology Laboratory protocol. BioStat® 2007 statistical analysis software was used for analysing Pearson correlation Indices. Results: a total of 226 spermograms presented 73,5% significant changes in all their parameters. Decreased rapidly progressive mobility (astenozoospermy) was observed in 65% and such downward trend was related to age (0,04% per year). Abnormalities in sperm morphology (teratozoospermy) were presented in 52,2%, having an upward trend (0,02% per year). Decreased sperm concentration was observed in 28% (0,01 × 106/mL per year decrease). Conclusions: there was a significant decrease in the study population's sperm quality which was greater than that reported in the literature. This was more relevant in the sense that as patients' age increased then the rapidly progressive mobility and morphology of their sperm became most affected, this being closely related to patients' fertilising capacity. 2009 © Federación Colombiana de Asociaciones de Obstetricia y Ginecología.
    Preview · Article · Jun 2009 · Revista colombiana de obstetricia y ginecología
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