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Genetic divergence and phylogenetic relationships of honey bee Apis mellifera (Hymenoptera: Apidae) populations from Greece and Cyprus using PCR – RFLP analysis of three mtDNA segments

Department of Biology, University of Patras, Rhion, West Greece, Greece
Apidologie (Impact Factor: 1.68). 07/2005; 36(3). DOI: 10.1051/apido:2005021

ABSTRACT

The genetic structure and phylogenetic relationships among six honey bee populations were studied using RFLP analysis on three PCR-amplified mtDNA gene segments (16s rDNA, CO I, and ND 5). The populations were sampled from various areas of Greece and Cyprus and correspond to Apis mellifera adami, A. m. macedonica, A. m. cecropia, and A. m. cypria races, based on origin (Ruttner, 1988). Seven, eight and seven restriction enzymes were found to have at least one recognition site at the 16s rDNA, CO I, and ND 5 segments, respectively. Seven different haplotypes were detected and diagnostic patterns enabled us to discriminate A. m. macedonica from the rest of the populations (races). The estimated net nucleotide sequence divergence among the populations examined was found to range from 0.00 to 1.18 with the highest value observed to be between A. m. macedonica and non-A. m. macedonica populations. The trees obtained (by UPGMA and Dollo parsimony methods) revealed that the most distant population was that of A. m. macedonica.

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    • "This approach allowed the identification of genetic profiles and their geographical distribution and has been widely applied for phylogeographical analyses (Carnaval et al., 2009; Resende et al., 2010; Thomé et al., 2010). Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) has successfully provided information on the genetic diversity of bees (Bouga et al., 2005; Brito and Arias, 2005; Collet et al., 2007). However, there is no information on the geographical distribution of mitochondrial lineages of Neotropical social wasps. "
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    • "The 16s rDNA gene segment was amplified according to Bouga et al. (2005). The PCR products were purified using a gel purification kit (Qiagen) and sequenced in both directions on an ABI Prism 3130 automated sequencer (Applied Biosystems) Fig. 1 Sampling locations in Turkey. "
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