Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, Tandogan, Ankara, Turkey
Anodic voltammetry of zolmitriptan at boron-doped diamond electrode
and its analytical applications
B. Uslu, D. Canbaz
Received July 23, 2009, accepted August 3, 2009
Pharmazie 65: 245–250 (2010)doi: 10.1691/ph.2010.9245
The electrooxidative behavior and determination of zolmitriptan at a boron-doped diamond electrode
were investigated using cyclic, linear sweep, differential pulse and square wave voltammetric tech-
niques. Zolmitriptan undergoes irreversible oxidation at a peak potential of about +0.9V (vs Ag/AgCl/3M
KCl). DPV and SWV techniques are proposed for the determination of zolmitriptan in phosphate
buffer at pH 3.03, which allows quantitation over the two different ranges (8×10−7–8×10−6M and
1×10−5–1×10−4M) in supporting electrolyte for both methods. A linear response was obtained in
phosphate buffer over two different ranges (6×10−7–8×10−6M and 1×10−5–1×10−4M) for spiked
serum samples at pH 3.03 for both techniques. The repeatability and reproducibility of the meth-
ods for all media were determined. The standard addition method was used in serum. Precision
and accuracy were also checked in all media. No electroactive interferences from the excipients and
endegenous substances were found in the pharmaceutical dosage form and the biological sample,
Boron-doped diamond (BDD) electrodes are becoming increas-
ingly attractive particularly in the field of electroanalysis due to
their unusual and extremely useful properties such as low and
stable background current (Spataru et al. 2002; Uslu and Ozkan
ous and non-aqueous solvent systems (Fujishima et al. 2002)
and good electroactivity towards certain organic species which
deactivate the surface of other conventional electrodes (Iniesta
BDD electrodes (Granger et al. 1999; Wirley et al. 2008; Zhao
et al. 2009; Uslu et al. 2008; Altun et al. 2009; Dogan-Topal
et al. 2007).
5-yl}methyl)-1,3-ozazolidin-2-one] is a selective serotonin
receptor agonist of the 1B and 1D subtypes. It is a triptan,
used in the acute treatment of migraine attacks with or without
aura and of cluster headaches (http:// www.medicinenet.com;
Peterlin et al. 2007). Migraine affects 18% of women and
6% of men. The significant impact of migraine results in a
huge burden for the individual, health services, and society.
Successful treatment of acute migraine attacks can reduce the
use of healthcare resources and improve health-related quality
of life (Oldman et al. 2002). The introduction of the trip-
tans in the 1990s revolutionized the treatment of migraine,
and a second-generation triptan, zolmitriptan, is highly effec-
tive in the oral treatment of acute migraine with or without
Spectrophotometry (Sankar et al. 2008; Raza et al. 2007;
Aydogmus and Inanlı 2007) and high-performance liquid
chromatography have been widely used for the quantitative
determination of triptans together with UV (Rao et al. 2005;
Yu et al. 2005), and mass spectrometry (Kılıc et al. 2007; Ding
et al. 2006; Chen et al. 2006; Zhang et al. 2004; Vishwanathan
et al. 2000) techniques. The sensitivity achieved by all these
procedures is highly satisfactory for the quantification of phar-
maceutical compounds. However, in some cases, a prior step is
required before quantification, involving extraction from mix-
tures with other compounds or from complex samples, which is
not economically feasible in routine analyses.
In this context, electroanalytical techniques have proved to be
compounds, since they are simple, cost little, and require rela-
tively short analysis times, without the need for derivatization
or time-consuming extraction steps. In addition to providing
high precision in pharmaceutical analyses, electroanalytical
Pharmazie 65 (2010)245
behavior of zolmitriptan on a BDD electrode was established and studied
for the first time. Zolmitriptan undergoes irreversible oxidation at positive
potentials. The BDD electrode was shown to be perfectly suitable for anal-
ysis of zolmitriptan using the DPV and SWV techniques. The advantage of
the BDD electrode is that it is highly stable and sensitive.
the oxidation of their indole moiety over the BDD electrode. This behavior
provides a useful tool for detection and quantification of drugs at low levels
in biological samples.
This paper is not intended to be a study of the pharmacodynamic properies
for sample collection and results may be of no significance. It showed the
possibility of monitoring this drug compound, making the method likely to
be useful for pharmacokinetic and pharmacodynamic purposes.
Acknowledgements: This work was supported by Ankara Univer-
sity Scientific Research Project Foundation (Grant Nos: 20030803037
and 20030803043), Turkey. The authors also gratefully acknowledge
AstraZeneca Ind. for supplying zolmitriptan standard samples.
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