In Vitro Regeneration of Castor (Ricinus Communis L.) Using Cotyledon Explants.

HortScience 02/2008; 43(1).
Source: OAI


An efficient plant regeneration protocol using cotyledon explants was established for castor (Ricinus communis L.), an important oilseed crop. Mature seed-derived cotyledon explants produced adventitious shoots when placed on Murashige and Skoog (MS) medium containing thidiazuron (TDZ). The rate of shoot regeneration was maximal (25 shoots per explant) when explants were cultured on shoot induction medium supplemented with 5 μM TDZ and preincubated in the dark for the first 7 days before transferring to the day/night cycle (16/8 h). Only the proximal ends of cotyledon explants produced adventitious shoots, although green calli were observed in cotyledon veins. After 4 weeks in culture, explants with well-developed shoot buds were transferred to MS medium without plant growth regulators for the shoot elongation and development. At 4 months after culture initiation, shoots (2 cm in length) were transferred to root induction medium (MS medium supplemented with 5 μM indole-3-butyric acid) where they developed roots in 4 to 6 weeks. Plantlets were transferred to soil and acclimatized to greenhouse conditions. Histological analysis showed the adventitious induction of the shoots originated from the cortical and epidermal cell layers of the cotyledon explants.

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    • "Green compact calli were often produced along the cotyledon vein but did not resulted in shoot regeneration. Similar type of result was also observed in castor by Ahn and Chen (2008). However callusing is less in the medium containing Kin. "
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    ABSTRACT: An efficient plant regeneration protocol was described for castor (Ricinus communis L.) using whole cotyledonary nodes as explant. Seeds were surface sterilized with 0.1% (w/v) mercuric chloride and germinated in growth regulator-free MS medium. Cotyledonary nodes were excised from 5-7 days old seedlings and were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of BAP, Kin singly or in combination with NAA. Use of BAP at 3.0 mg.l -1 induced the highest frequency (85%) of shoot induction as well as maximum number of shoots per explant (12.56). Proliferated shoot clusters were elongated in 1.0 mg.l -1 BAP in combination with 0.25 mg.l -1 GA 3 . For root induction, in vitro shoots were transferred to rooting media containing NAA or IBA. The highest rooting frequency (87.5%) as well as highest number of roots (10.5) was observed in MS medium supplemented with 1.0 mg.l -1 NAA. Regenerated plantlets were acclimatized successfully in the growth room for further development.
    Full-text · Article · Mar 2010 · Australian Journal of Crop Science
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    Full-text · Article · Jan 2010
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    ABSTRACT: Successful in vitro plant regeneration protocol has been described for fusarium wilt resistant castor (Ricinus communis L.) parental line SKP-84 through apical meristem. Shoot apex containing apical meristems, were excised from 5-7 days old in vitro grown seedlings and cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of cytokinins either alone or in combination. Kinetin had marked effect on shoot initiation and shoot quality. Kinetin (2.325µM) in combination with BAP (1.111µM) produced maximum number of shoot (10.33) and shoot length (5.20 cm). In vitro produced shoots were transferred to rooting media containing half strength MS basal media supplemented with NAA at various concentrations. Well developed roots appeared in media supplemented with 0.537µM NAA (69.00 %). Rooted plants were transferred to the pots containing vermicompost with 64.5% survival rate in hardeninng. No genetic variation was detected in plantlets as revealed by RAPD at different stages proved the genetically stability during in vitro plant regeneration through apical meristem culture.
    Full-text · Article · Jan 2013
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