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The cardiac and haemostatic effects of dietary hempseed


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Despite its use in our diet for hundreds of years, hempseed has surprisingly little research published on its physiological effects. This may have been in the past because the psychotropic properties wrongly attributed to hemp would complicate any conclusions obtained through its study. Hemp has a botanical relationship to drug/medicinal varieties of Cannabis. However, hempseed no longer contains psychotropic action and instead may provide significant health benefits. Hempseed has an excellent content of omega-3 and omega-6 fatty acids. These compounds have beneficial effects on our cardiovascular health. Recent studies, mostly in animals, have examined the effects of these fatty acids and dietary hempseed itself on platelet aggregation, ischemic heart disease and other aspects of our cardiovascular health. The purpose of this article is to review the latest developments in this rapidly emerging research field with a focus on the cardiac and vascular effects of dietary hempseed.
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Rodriguez-Leyva and Pierce Nutrition & Metabolism 2010, 7:32
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The cardiac and haemostatic effects of dietary
Delfin Rodriguez-Leyva
and Grant N Pierce*
Despite its use in our diet for hundreds of years, hempseed has surprisingly little research published on its physiological
effects. This may have been in the past because the psychotropic properties wrongly attributed to hemp would
complicate any conclusions obtained through its study. Hemp has a botanical relationship to drug/medicinal varieties
of Cannabis. However, hempseed no longer contains psychotropic action and instead may provide significant health
benefits. Hempseed has an excellent content of omega-3 and omega-6 fatty acids. These compounds have beneficial
effects on our cardiovascular health. Recent studies, mostly in animals, have examined the effects of these fatty acids
and dietary hempseed itself on platelet aggregation, ischemic heart disease and other aspects of our cardiovascular
health. The purpose of this article is to review the latest developments in this rapidly emerging research field with a
focus on the cardiac and vascular effects of dietary hempseed.
Cannabis sativa L. is an annual plant in the Cannabaceae
family. It has been an important source of food, fiber,
medicine and psychoactive/religious drug since prehis-
toric times [1]. Cannabis is mentioned as a medication in
ancient Egyptian medical texts: Ramesseum III Papyrus
(1700 B.C.), Eber's Papyrus (1600 B.C.), the Berlin Papy-
rus (1300 B.C.), and the Chester Beatty VI Papyrus (1300
B.C.) [1,2].
Two main types of Cannabis Sativa L. must be distin-
guished, the drug and non-drug types. The first is also
known as marijuana, hashish or Cannabis tincture and
contains Δ9-Tetrahydrocannabinol (THC) in concentra-
tions between 1-20%, high enough to exhibit psychoactiv-
ity. The second type of Cannabis Sativa L. is industrial
hemp with THC concentrations < 0.3% so it has no psy-
choactive properties [3,4].
Canada, Australia, Austria, China, Great Britain,
France and Spain are among the most important agricul-
tural producers of hempseed. In the United States, it is
not legal to cultivate hempseed. This is primarily because
many believe that by legalizing hemp they may lead to a
legalization of marijuana [5]. Other governments have
accepted the distinction between the two types of Canna-
bis and, while continuing to penalize the growing of mar-
ijuana, have legalized the growing of industrial hemp [5].
Hempseed possesses excellent nutritional value. It is
very rich in essential fatty acids (EFAs) and other polyun-
saturated fatty acids (PUFAs). It has almost as much pro-
tein as soybean and is also rich in Vitamin E and minerals
such as phosphorus, potassium, sodium, magnesium, sul-
fur, calcium, iron, and zinc [6,7]. The nutrient profile of
hempseed is shown in Table 1. Hempseed oil contains all
of the essential amino acids and also contains surprisingly
high levels of the amino acid arginine, a metabolic pre-
cursor for the production of nitric oxide (NO), a molecule
now recognized as a pivotal signaling messenger in the
cardiovascular system that participates in the control of
hemostasis, fibrinolysis, platelet and leukocyte interac-
tions with the arterial wall, regulation of vascular tone,
proliferation of vascular smooth muscle cells, and
homeostasis of blood pressure [8]. In a study that
included 13 401 participants, 25 years and older from the
Third National Health Nutrition and Examination Sur-
vey, an independent relationship was shown between the
dietary intake of L-arginine and levels of C-Reactive pro-
tein [9], a marker strongly correlated with the risk of car-
diovascular disease (CVD) [10]. The results of this
populated-based study suggested that individuals may be
able to decrease their risk for CVD by following a diet
that is high in arginine-rich foods [9]. Dietary hempseed
* Correspondence:
1 Department of Physiology, University of Manitoba and Institute of
Cardiovascular Sciences, St Boniface Hospital Research Centre, 351 Tache
Avenue, Winnipeg, Manitoba, R2H 2A6, Canada
Full list of author information is available at the end of the article
Rodriguez-Leyva and Pierce Nutrition & Metabolism 2010, 7:32
Page 2 of 9
Table 1: Nutrient profile of hempseed*.
Nutrient Units Value per 100
Nutrient Units Value per 100
Energy kcal 567 Lipids
Energy kJ 2200 Saturated fat g 3.3
Protein g 24.8 16:0 g 3.44
Total lipid (fat) g 35.5 18:0 g 1.46
Ash g 5.6 20:0 g 0.28
Carbohydrates g 27.6 Monounsaturated
Fiber, total dietary g 27.6 18:1n9 g 9
Digestable fiber g 5.4 Total
g 22.2 18:2n6 g 56
Moisture g 6.5 18:3n6 g 4
Glucose g 0.30 18:3n3 g 22
Fructose g 0.45 18:4n3 g 2
Lactose g <0.1 Cholesterol mg 0
Maltose g <0.1 Amino acids
Tryptophan g 0.20
Minerals Threonine g 0.88
Calcium, Ca mg 145 Isoleucine g 0.98
Iron, Fe mg 14 Leucine g 1.72
Magnesium, Mg mg 483 Lysine g 1.03
Rodriguez-Leyva and Pierce Nutrition & Metabolism 2010, 7:32
Page 3 of 9
is also particularly rich in the omega-6 fatty acid linoleic
acid (LA) and also contains elevated concentrations of
the omega-3 fatty acid α-linolenic acid (ALA). The
LA:ALA ratio normally exists in hempseed at between
2:1 and 3:1 levels. This proportion has been proposed to
be ideal for a healthy diet [11]. Other rich sources of LA
[12,13] are listed in Table 2.
The long chain PUFA that is found in the body ulti-
mately originates from the diet and through elongation
and desaturation of their dietary precursors, ALA and
LA. Both families of fatty acids, n-3 and n-6, share and
compete for the same enzymes (Δ6-desaturase, Δ5-desat-
urase, and elongases) in their biosynthetic pathways. The
Δ6-desaturase enzyme is the rate-limiting step [9]. Fol-
lowing its metabolism, LA can be converted into arachi-
donic acid whereas ALA will be converted into the long
chain fatty acids, eicosapentaenoic acid (EPA) and doco-
sahexaenoic acid (DHA) (Figure 1). A high LA intake
interferes with the desaturation and elongation of ALA
[14]. Therefore, theorically, a lower ratio of omega-6/
omega-3 fatty acids is more advantageous in reducing the
risk of many of the chronic diseases of high prevalence in
Western societies. The ratio of ω-6 to ω-3 fatty acids
ranges from 20-30:1 in Western societies instead of the
traditional (historic) range of 1-2:1 on which human
beings evolved [15]. This is thought to be closely associ-
ated with chronic diseases like coronary artery disease,
hypertension, diabetes, arthritis, osteoporosis, inflamma-
tory and autoimmune disorders and cancer.
Phosphorus, P mg 1160 Methionine g 0.58
Potassium, K mg 859 Cystine g 0.41
Sodium, Na mg 12 Phenylalanine g 1.17
Zinc, Zn mg 7 Tyrosine g 0.82
Copper, Cu mg 2 Valine g 1.28
Manganese, Mn mg 7 Arginine g 3.10
Selenium, Se mcg <0.02 Histidine g 0.71
Vitamins Alanine g 1.28
Vitamin C mg 1.0 Aspartic acid g 2.78
Thiamin mg 0.4 Glutamic acid g 4.57
Riboflavin mg 0.11 Glycine g 1.14
Niacin mg 2.8 Proline g 1.15
Vitamin B-6 mg 0.12 Serine g 1.27
Vitamin A IU 3800
Vitamin D UI 2277.5
Vitamin E mg 90.00
* Adapted from reference 6 and 7. Data based on Finola variety of hempseed.
Table 1: Nutrient profile of hempseed*. (Continued)
Rodriguez-Leyva and Pierce Nutrition & Metabolism 2010, 7:32
Page 4 of 9
Hempseed is also a rich and unusual source of the poly-
unsaturated fatty acid gamma linolenic acid (GLA)
(18:3n6) to the body. Additionally, another important
biological metabolite of ALA and LA, stearidonic acid
(18:4n3; SDA) is also present in hempseed oil (Figure 1).
Both can inhibit inflammatory responses [16,17].
Recently, many studies have demonstrated that dietary
interventions can play a central role in the primary and
secondary prevention of several diseases. The PUFAs
derived from fish, EPA and DHA, have been extensively
studied. Based on the close relation between the path-
ways that metabolize ALA and LA, and the capacity of
both to be converted into long chain fatty acids, plant
sources of ALA (i.e. flaxseed, canola and others) have
begun to attract more scientific attention for their poten-
tial to improve our health. However, because of legal reg-
ulations, lack of knowledge and some confusion about the
differences between fiber hemp and marijuana, the
growth of hempseed research has been slower than
expected. In view of its long history of dietary usage, it is
surprising that research on the effects of dietary hemp-
seed in animal and humans has been limited. Further-
more, because of its expected nutritional value and the
hypothetical benefits of LA and ALA against a variety of
health disorders, a better understanding of the appropri-
ate doses and presentation (oil, seed, etc) of hempseed
should represent useful health-related information. It is
important to point out that dietary hempseed as an
energy containing food item introduces changes in the
fatty acid composition of the diet and will inevitably
replace other dietary components under an isocaloric
condition. Previously some [18] but not all authors [19]
have found differences in body weight after the adminis-
tration of 30 ml/d of hempseed oil for four to eight weeks
in humans. Finally, an identification of the target patient
population (age, clinical condition, co-morbidities, etc)
that may benefit the most from a supplementation of
hempseed in the diet would also be important informa-
Animal Data
The biochemical metabolism of omega-6 fatty acids like
LA produces eicosanoids in the body. Eicosanoids are
biologically active and contribute to the formation of
thrombi and atheromas and shifts the physiological state
to one that is prothrombotic and proaggregatory, with
increases in blood viscosity, vasospasm, and vasocontric-
tion and decreases in bleeding time [15]. Hempseed is
rich in LA content. Therefore, hempseed has received
research attention for its effects on platelet aggregation.
Richard et al [20] reported that diets supplemented
with 5% and 10% hempseed (wt/wt) for 12 weeks resulted
in a significant increase in total plasma PUFAs in rats.
ALA and LA levels increased significantly in a concentra-
tion-dependent manner [20]. Dietary hempseed supple-
mentation also resulted in a significant inhibition of
platelet aggregation and a lower rate of aggregation. This
is an important result with physiological and pathological
implications. As we have become increasingly aware of
the importance of blood clots to the initiation of myocar-
Table 2: Rich sources of the essential fatty acid linoleic acid*.
Source of LA LA (g/100 g) ALA (g/100 g) Ratio n6/n3
Safflower oil 73 0.4 >100
Corn oil 57 1 57
Hempseed Oil 56 22 2.5
Cottonseed oil 50 0.2 >100
Soybean oil 50 8 6.2
Sesame oil 40 0.3 >100
Black walnuts 37 2 18.5
English walnuts 35 6.8 5.1
Sunflower seeds 30 0.06 >100
Brazil nuts 25 0.01 >100
Margarine 22 2.1 10.4
Pumpkin and squash seeds 20 0.12 >100
Spanish peanuts 16 0.01 >100
Peanut butter 15 0.08 >100
Almonds 10 0.06 >100
*Adapted from reference [12] and [13]
Rodriguez-Leyva and Pierce Nutrition & Metabolism 2010, 7:32
Page 5 of 9
dial infarctions and strokes, the capacity of a dietary
intervention like hempseed to inhibit clot formation has
obvious appeal. However, if excessive bleeding is an
expected event (as would be the case during surgery), it
becomes essential for the physician/surgeon to know of a
prior history of dietary hempseed usage.
These data on the effects of dietary hempseed on plate-
let aggregation have been extended into hypercholester-
olemic conditions by Prociuk and colleagues [21]. They
have shown that rabbits fed a high cholesterol diet for
eight weeks exhibit an enhanced platelet aggregation [21].
However, when 10% hempseed was supplemented to the
diet together with the high cholesterol diet, these hyperc-
holesterolemic animals displayed normal platelet aggre-
gation values. This normalization was not related to any
correction of the elevated plasma cholesterol levels but
was related in part to the increased levels of plasma
gamma-linolenic acid [21]. Because most patients at high
risk for coronary heart disease are hypercholesterolemic,
these findings have important potential for treating or
preventing cardiovascular diseases.
Two other studies have been focused on the capacity of
hempseed for altering cardiac function before and after
an ischemic event [22,23]. Both studies have shown no
effects of a hempseed-supplemented diet on basal cardiac
contractile function or electrical activity before ischemia
[22,23]. However, the data on the effects of dietary hemp-
seed on cardiac performance post-ischemia is less consis-
tent. Al-Khalifa and colleagues [23] reported that hearts
from rats fed a 5% or 10% hempseed supplemented diet
for 12 weeks exhibited significantly better post-ischemic
recovery of maximal contractile function and enhanced
rates of tension development and relaxation during rep-
erfusion than hearts from the control group. The authors
found that these hearts were not protected from the
occurrence of premature contractions, nor were the
increases in resting tension altered during ischemia or
reperfusion [23]. This beneficial effect of hempseed on
post-ischemic cardiac performance may be species spe-
cific. The same lab found that supplementation of the diet
with 10% hempseed in rabbits did not show any beneficial
effects on left ventricular end-diastolic pressure
(LVEDP), left ventricular developed pressure (LVDP),
arrhythmia incidence and arrhythmia duration during
ischemia and reperfusion [22]. Some limitations of the
study related to the duration of the dietary intervention
(8 weeks as opposed to 12 weeks) and sample size may
have influenced the capacity for the dietary hempseed to
protect the heart during ischemic insult [22].
Clinical Data
The actions of dietary hempseed in humans have only
been studied to a limited extent. Fatty acid bioavailability
from hempseed oil was recently studied in comparison to
two other dietary oils (fish and flaxseed) [24]. Hempseed
and hempseed oil is enriched in LA and GLA. Eighty-six
healthy subjects completed a 12 week dietary supplemen-
tation with 2 g/day of these oils. The hempseed interven-
tion did not significantly increase the concentration of
LA, GLA or any other fatty acid in the plasma of the sub-
jects, nor did it change the level of plasma total choles-
terol (TC), high density cholesterol (HDL-C), low density
cholesterol (LDL-C) or triglycerides (TG) [24]. Both flax-
seed and fish oils did induce significant changes in circu-
lating fatty acid species associated with their respective
oils (ALA for flaxseed; EPA and DHA for fish oil) [24].
Supplementation with hempseed oil also did not induce
any change in collagen- or thrombin-stimulated platelet
aggregation or in the levels of circulating inflammatory
markers [24]. It was suggested that the lack of effects may
be related to the dose used [24]. This hypothesis has been
supported by data obtained in another dietary interven-
tion that used higher doses of hempseed (30 ml/day) [18].
In this randomized, double-blinded, crossover design
trial, hempseed and flaxseed oils were compared at the
same doses. After 4 weeks of supplementation, the hemp-
seed intervention increased the concentrations of both
LA and GLA in serum cholesteryl esters (CE) and TG.
The flaxseed intervention resulted in higher serum CE
Figure 1 Biochemical pathway for linolenic acid and α-linolenic
acid transformation. ALA = α-linolenic acid; ARA = arachidonic acid;
DGLA = dihomo γ-linolenic acid; DHA = docosahexaenoic acid; DPA =
docosapentaenoic acid; EPA = eicosapentaenoic acid; GLA = γ-linolen-
ic acid; LA = linoleic acid.
18:3 (GLA) 18:4
22:5 (DPA)
24:4 24:5
18:3 (ALA)
20:5 (EPA)
22:6 (DHA)
n-6 n-3
18:2 (LA)
20:4 (ARA)
22:5 (DPA)
Rodriguez-Leyva and Pierce Nutrition & Metabolism 2010, 7:32
Page 6 of 9
and TG concentrations of ALA. However, a statistically
significant decrease in GLA concentrations was observed
during this period of intervention. Importantly, the pro-
portion of arachidonic acid in CE was lower after the
flaxseed diet than after the hempseed supplementation
but this was not statistically significant. However, the
hempseed supplements resulted in a lower total choles-
terol:HDL cholesterol ratio. A higher total-to-HDL cho-
lesterol ratio has been found in association with coronary
heart disease [25]. However, no significant differences
were found between the effects of flaxseed and hempseed
oils in terms the fasting serum total or lipoprotein lipid
levels, plasma glucose levels, or insulin or hemostatic fac-
tors [18]. Callaway and colleagues [19], using 30 ml/day of
hempseed oil, conducted a 20-week randomized, single-
blind crossover study in 20 patients with atopic dermati-
tis, and found that the levels of both essential fatty acids,
LA and ALA, and GLA increased in all lipid fractions
after using hempseed oil, with no significant increases of
arachidonic acid in any lipid fractions. Moreover, atopic
dermatitis symptoms were improved after the interven-
tion with hempseed oil [19].
These results emphasize the importance of using
higher doses of hempseed oil if significant increases in
fatty acid species are to be achieved. Clearly, the ingestion
of two large capsules of hempseed daily (as most people
in the general public may ingest), is insufficient to achieve
a desired increase in LA or GLA levels in the plasma [24].
Much larger doses are required to induce beneficial phys-
iological effects. However, this may not be possible to
achieve currently in the general population. If 10-15
times the amount used by Kaul and co-workers [24] is
required to achieve a significant increase in plasma fatty
acid levels, it would be unpractical to expect the general
public to ingest 20-30 capsules of hempseed per day. This
is a significant problem that the food and supplement
industry must address in the future if hempseed is to be
considered a realistic dietary approach to healthy living.
Supplementing the diet with tablespoons of hemp oil in
addition to hemp capsules as well as ingesting foods that
contain these omega-3 fatty acids may be the optimal way
to obtain them.
Linoleic acid and heart disease: New research fields
for hempseed
Hempseed is a rich source of LA and others nutrients.
The specific pathologies or conditions in which it can be
used effectively are in need of more research but the data
presently available suggest that LA may have beneficial
effects in certain cardiovascular circumstances.
Effects on cholesterol levels
Iacono et al [26] reported that a high LA based diet
(10.8%) decreased total cholesterol by 15% and LDL-C by
22%, without producing significant changes in plasma
HDL-C after 6 weeks of dietary intervention in 11 healthy
middle aged, male subjects. Apolipoprotein B decreased
by 37% whereas apolipoprotein A-I increased by 24% in
the group of individuals supplemented with this diet [26].
In a multiple crossover design that included 56 normo-
lipemic, healthy subjects, Zock and colleagues [27] found
that those who received the LA supplemented dietary
intervention for three weeks (2.0% of total energy intake
as LA) obtained lower levels of serum LDL-C, and higher
HDL-C levels when compared with subjects who received
its hydrogenation products elaidic (trans-Cl8:ln9) and
stearic acid (C18:O). Recently, Mensink et al [28]
employed a meta-analysis that included 60 controlled tri-
als to show that polyunsaturated fat (mainly LA) reduces
LDL-C, triglycerides and increases HDL-C. However,
others have shown that healthy individuals supplemented
for 4 weeks with hempseed exhibited a lower total-to-
HDL cholesterol ratio [24]. A higher total:HDL choles-
terol ratio is associated with coronary heart disease and
has a worse prognosis after a myocardial infarction
[29,30]. Clearly, the issue is not resolved yet. The popula-
tion studied (healthy vs clinically compromised), the dos-
ages of hempseed used, the presentation administered
(whole hempseed vs milled hempseed vs hemp oil vs
purified LA), the duration of the dietary intervention, the
composition of the diet, are all factors that may be critical
in producing the effects (of lack of effects) in these stud-
ies. More research is needed in order to understand if
these specific conditions influence cardiovascular effi-
cacy and to understand which metabolic factors are most
sensitive (hypertriglyceridemia, hypercholesterolemia,
low HDL-C, or other hyperlipoproteinemias) to this kind
of dietary intervention.
Effects on high blood pressure
Results reported by The International Study of Macro-
Micronutrients and Blood Pressure, a cross-sectional epi-
demiological study that included 4680 individuals, sug-
gested that dietary LA intake may contribute to
prevention and control of high blood pressure [31]. Other
small studies have found that supplementation with LA (4
g-23 g/day) decreased blood pressure after 4 weeks of
dietary intervention [32,33]. However, these promising
results are in conflict with another study that reported no
association between LA intake and lower blood pressure
levels [34]. Studies using hempseed as a source of LA for
hypertensive patients have not been conducted. It is also
important to note that the consequences of these kinds of
diets on arterial stiffness and vascular perfusion charac-
teristics are unknown. The additional effects of these
diets on ventricular hypertrophy that develops secondary
to high blood pressure is not known nor are the effects
when hempseed is supplemented with an antihyperten-
Rodriguez-Leyva and Pierce Nutrition & Metabolism 2010, 7:32
Page 7 of 9
sive medication. The potential for hempseed to alter drug
kinetics in the body has not been studied.
Effects on atherosclerosis
Almost three decades ago, Cornwell and Panganamala
postulated that an intracellular deficiency in essential
fatty acids plays a central role in the atherogenic process
[35]. Recently, Das [36] showed how a defect in the activ-
ity of Δ6 and Δ5 desaturases may be a factor in the initia-
tion and progression of atherosclerosis. He also provided
evidence that low-grade systemic inflammatory condi-
tions are also essential fatty acids deficient states [36].
With our current understanding of the close relationship
that infectious disease and inflammation has with athero-
genesis [37,38], it is not difficult to predict that foods with
an optimal LA-ALA ratio will reduce inflammation under
ideal dietary conditions and it may thereby attenuate ath-
erosclerotic heart disease. Unfortunately, the effects of
LA on atherosclerosis are not completely clear. Arachi-
donic acid can be derived from LA. This can be con-
verted to prothrombotic and proinflammatory
prostaglandins. However, changes in dietary LA within
the usual dietary range do not appreciably alter arachi-
donic acid levels [39,40]. Consistent with this, some have
suggested that LA could have anti-inflammatory effects
mediated by biochemical pathways that do not involve
the cyclooxygenase pathway [41]. Presently, the random-
ized, controlled trials that address this topic have not
been able to distinguish between the effects of omega-3
and omega-6 fatty acids [42]. Both have had beneficial
effects by decreasing plasma levels of soluble TNF recep-
tor 1 and 2, indicators of TNF activity [42].
Surprisingly, studies of the effects on atherosclerotic
heart disease of dietary hempseed supplementation in
animals or humans have not been completed. This type of
study has been successfully completed using flaxseed as a
dietary intervention [43,44]. It would also be important to
determine if the LA content of hempseed (and not its
ALA content) is responsible for decreasing inflammatory
markers and the systemic atherosclerotic process in gen-
Coronary heart disease
A meta-analysis of data from 25 case-control studies
strongly suggested that a lower tissue content of LA is
associated with increased coronary heart disease risk
[45]. More importantly, this study did not show an associ-
ation between AA tissue content and the risk for coro-
nary artery disease. The results from randomized
controlled trials have not been consistent either. Some
[46,47] but not all [48,49] have found reductions in coro-
nary risk with the use of an LA diet intervention. In a
recent review, Harris [45] states that reducing LA intakes
to less than 5% energy would be likely to increase the risk
for coronary heart disease whereas higher intakes should
be beneficial even in conditions without clinical evidence
of adverse effects.
What we do not know about the effects of dietary
As discussed earlier in this paper, there is a lack of knowl-
edge regarding the usefulness of hempseed or LA in dif-
ferent aspects related to cardiovascular diseases. It is
important to identify not only what we presently know
about dietary hempseed but also what is not known. The
animal data lacks systematic information about the action
of hempseed on myocardial infarctions, hypertension,
atherosclerosis, markers of inflammation and arrhyth-
mias. Similarly, we need to know more about the effects
of this plant on the circulating lipid profile. Primary and
secondary cardiovascular prevention trials using hemp-
seed as a source of LA have not been performed. In gen-
eral, we need to understand better the bioavailability of
fatty acids like LA and GLA from dietary hempseed as a
function of the age or sex of the subject, or as a function
of the dosage of hempseed employed. Other dietary inter-
ventions (i.e. flaxseed) are sensitive to these variables
[50,51] so it is not unrealistic to hypothesize that the
delivery of hempseed will be influenced by these variables
as well. It will also be important to identify if the hypoten-
sive effects attributed to LA can be reproduced by dietary
hempseed. As discussed previously, the capacity of LA
and/or hempseed to affect ventricular hypertrophy sec-
ondary to high blood pressure, human atherosclerosis,
inflammation, as well as the co-morbidities associated
with cardiovascular diseases (like metabolic syndrome,
diabetes mellitus, insulin resistance, obesity, heart failure
or arrhythmias) still need to be determined in carefully
controlled clinical trials.
The data discussed above supports the hypothesis that
hempseed has the potential to beneficially influence heart
disease. A mix of legal issues and misunderstandings has
slowed research progress in this area but enough data
presently exists to argue strongly for the continued inves-
tigation into the therapeutic efficacy of dietary hemp-
seed. There remain many questions regarding the
cardiovascular effects of hempseed that demand scien-
tific answers in order to definitively establish this food as
a preventive or therapeutic dietary intervention. Cardio-
vascular patients may not be the only subjects who bene-
fit from this research. Furthermore, only time will tell if
other diseases that have an immunological, dermatologi-
cal, neurodegenerative basis may also benefit from this
new nutritional intervention.
Rodriguez-Leyva and Pierce Nutrition & Metabolism 2010, 7:32
Page 8 of 9
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
Both authors contributed to the creation, literature review and writing of this
The work was supported through a grant from the Canadian Institutes for
Health Research. The indirect costs of this research were supported by the St
Boniface Hospital and Research Foundation. Dr Rodriguez Leyva was a Visiting
Scientist of the Heart and Stroke Foundation of Canada.
Author Details
1Department of Physiology, University of Manitoba and Institute of
Cardiovascular Sciences, St Boniface Hospital Research Centre, 351 Tache
Avenue, Winnipeg, Manitoba, R2H 2A6, Canada and 2Cardiovascular Research
Division, V.I. Lenin Universitary Hospital, s/n Lenin Avenue, Holguin, 80100,
1. Russo EB: History of cannabis and its preparations in saga, science,
andsobriquet. Chem Biodivers 2007, 4:1614-48.
2. Manniche L: An ancient Egyptian herbal. Third University of Texas Press
Printing; 1989.
3. Ross SA, Mehmedic Z, Murphy TP, Elsohly MA: GC-MS analysis of the
totalΔ9-THC content of both drug-and fiber-type cannabis seeds. J
Anal Toxicol 2000, 24:715-717.
4. Holler JM, Bosy TZ, Dunk ley CS, Levine B, Past MR, Jacobs A: Delta9-
tetrahydrocannabinol content of commercially available hemp
products. J AnalToxicol 2008, 32:428-32.
5. West DP: Hemp and Marijuana: Myths & Realities. 1998 [http://]. North AmericanIndustrial
Hemp Council, INC April 8,2009
6. Callaway JC: Hempseed as a nutritional resource: An overview.
Euphytica 2004, 140:65-72.
7. Welcome to Finola®. February 26 2009 [].
April16, 2009
8. Napoli C, Ignarro LJ: Nitric oxide and pathogenic mechanisms involved
inthedevelopment of vascular diseases. Arch Pharm Res 2009,
9. Wells BJ, Mainous AG, Everett CJ: Association Between dietaryarginine
and C-reactive protein. Nutrition 2005, 21:125-30.
10. Ridker PM, Cushman M, Stampfer MJ, Tracy RP, Hennekens CH:
Inflammation, aspirin, and the risk of cardiovascular disease in
apparently healthy men. N Engl J Med 1997, 336:973-9.
11. Holub BJ: Clinical nutrition: 4. Omega-3 fatty acids in
cardiovascularcare. CMAJ 2002, 166:608-15.
12. Ensminger AH, Konlande JE: Fats and other lipids. In Foods & nutrition
encyclopedia Edited by: Ensminger AH, Konlande JE. CRC Press; 1993:691.
13. USDA National Nutrient Database for Standard Reference, Release 22.
October 10 2009 [].
February 18, 2010
14. Emken EA, Adlof RO, Rakoff H, Rohwedder WK: Metabolism of
deuterium-labeled linolenic, linoleic, oleic, stearic and palmitic acid in
humansubjects. In Synthesis and application of
isotopicallylabeledcompounds Edited by: Baillie TA, Jones JR. Amsterdam:
Elsevier Science Publishers; 1988:713-716.
15. Simopoulos AP: The importance of the omega-6/omega-3 fatty acid
ratio incardiovascular disease and other chronic diseases. Exp Biol Med
(Maywood) 2008, 233:674-88.
16. Chang CS, Sun HL, Lii CK, Chen HW, Chen PY, Liu KL: Gamma-linolenic
acid inhibits inflammatory responses by regulating NF-kappaB and AP-
1 activationin lipopolysaccharide-induced RAW 264.7 macrophages.
Inflammation 2010, 33:46-57.
17. Horia E, Watkins BA: Comparison of stearidonic acid and alpha-
linolenicacid on PGE2 production and COX-2 protein levels in MDA-
MB-231 breast cancer cell cultures. J Nutr Biochem 2005, 16:184-92.
18. Schwab US, Callaway JC, Erkkilä AT, Gynther J, Uusitupa MI, Järvinen T:
Effects of hempseed and flaxseed oils on the profile of serum lipids,
serum total andlipoprotein lipid concentrations a nd haemostatic
factors. Eur J Nutr 2006, 45:470-7.
19. Callaway J, Schwab U, Harvima I, Halonen P, Mykkänen O, Hyvönen P,
Järvinen T: Efficacy of dietary hempseed oil in patients with atopic
dermatitis. J Dermatolog Treat 2005, 16:87-94.
20. Richard MN, Ganguly R, Steigerwald SN, Al-Khalifa A, Pierce GN: Dietary
hempseed reduces platelet aggregation. J Thromb Haemost 2007,
21. Prociuk MA, Edel AL, Richard MN, Gavel NT, Ander BP, Dupasquier CM,
Pierce GN: Cholesterol-induced stimulation of platelet aggregation is
prevented byahempseed-enriched diet. Ca n J Physiol Pharmacol 2008,
22. Prociuk M, Edel A, Gavel N, Deniset J, Ganguly R, Austria J, Ander B, Lukas
A, Pierce G: The effects of dietary hempseed on cardiac ischemia/
reperfusioninjury in hypercholesterolemic rabbits. Exp Clin Cardiol
2006, 11:198-205.
23. Al-Khalifa A, Maddaford TG, Chahine MN, Austria JA, Edel AL, Richard MN,
Ander BP, Gavel N, Kopilas M, Ganguly R, Ganguly PK, Pierce GN: Effect
ofdietary hempseed intake on cardiac ischemia-reperfusion injury. Am
J Physiol RegulIntegr Comp Physiol 2007, 292:R1198-203.
24. Kaul N, Kreml R, Austria JA, Richard MN, Edel AL, Dibrov E, Hirono S, Zettler
ME, Pierce GN: A comparison of fish oil, flaxseed oil and hempseed oil
supplementation on selected parameters of cardiovascular health in
healthy volunteers. J Am Coll Nutr 2008, 27:51-8.
25. Kinosian B, Glick H, Preiss L, Puder KL: Cholesterol and coronary
heartdisease: predicting risks in men by changes in levels and ratios. J
Investig Med 1995, 43:443-450.
26. Iacono JM, Dougherty RM: Lack of effect of linoleic acid on thehigh-
density-lipoprotein-cholesterol fraction of plasma lipoproteins. Am J
Clin Nutr 1991, 53:660-4.
27. Zock PL, Katan MB: Hydrogenation alternatives: effects of trans
fattyacids and stearic acid versus linoleic acid on serum lipids and
lipoproteins inhumans. J Lipid Res 1992, 33:399-410.
28. Mensink RP, Zock PL, Kester AD, Katan MB, et al.: Effects of dietaryfatty
acids and carbohydrates on the ratio of serum total to HDL cholesterol
and on serumlipids andapolipoproteins: a meta-analysis of 60
controlled trials. Am J Clin Nutr 2003, 77:1146-55.
29. Marchioli R, Avanzini F, Barzi F: GISSI-Prevenzione Investigators.
Assessment of absolute risk of death after myocardial infarction by use
ofmultiple-risk-factor assessment equations: GISSI-Prevenzione
mortality risk chart. Eur Hear t J 2001, 22:2085-103.
30. Stampfer MJ, Sacks FM, Salvini S, Willett WC, Hennekens CH: Aprospective
study of cholesterol, apolipoproteins, and the risk of myocardial
infarction. N Engl J Med 1991, 325:373-381.
31. Miura K, Stamler J, Nakagawa H, International Study ofMacro-
Micronutrients and Blood Pressure Research Group: Relationship of
dietary linoleic acid toblood pressure. The International Study of
Macro-Micronutrients and BloodPressure. Hypertension 2008,
32. Heagerty AM, Ollerenshaw JD, Robertson DI, Bing RF, Swales JD:
Influenceofdietary linoleic acid on leucocyte sodium transport and
blood pressure. BMJ 1986, 293:295-297.
33. Sacks FM, Stampfer MJ, Monoz A, McManus K, Canessa M, Kass EH: Effect
oflinoleic and oleic acids on blood pressure, blood viscosity,
anderythrocyte cationtransport. J A m Coll Cardiol 1987, 6:179-185.
34. Salonen JT, Salonen R, Ihanainen M, Parviainen M, Seppänen R, Kantola M,
Seppänen K, Rauramaa R: Blood pressure, dietary fats, and antioxidants.
Am J Clin Nutr 1988, 48:1226-1232.
35. Cornwell DG, Panganamala RV: Atherosclerosis: an
intracellulardeficiency in essential fatty acids. Prog Lipid Res 1981,
36. Das UN: A defect in the activity of Delta6 and Delta5 desaturases may
bea factor in the initiation and progression of atherosclerosis.
Prostaglandins LeukotEssent Fatty Acids 2007, 76:251-68.
37. Hu H, Pierce GN, Zhong G: The atherogenic effects of chlamydia
aredependent on serum cholesterol and specific to Chlamydia
pneumoniae. J Clin Invest 1999, 103:747-53.
38. Hirono S, Dibrov E, Hurtado C, Kostenuk A, Ducas R, Pierce GN:
Chlamydiapneumoniae stimulates proliferation of vascular smooth
Received: 10 September 2009 Accepted: 21 April 2010
Published: 21 April 2010
This article is available from:© 2010 Rodriguez-Leyva and Pierce; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Nutrition & Metabolism 2010, 7:32
Rodriguez-Leyva and Pierce Nutrition & Metabolism 2010, 7:32
Page 9 of 9
muscle cells throughinduction of endogenous heat shock protein 60.
Circ Res 2003, 93:710-6.
39. Goyens PL, Spilker ME, Zock PL, Katan MB, Mensink RP: Conversion
ofalpha-linolenic acid in humans is influenced by the absolute
amounts ofalpha-linolenicacid and linoleic acid in the diet and not by
their ratio. Am J Clin Nutr 2006, 84:44-53.
40. Hussein N, Ah-Sing E, Wilkinson P, Leach C, Griffin BA, Millward DJ: Long-
chain conversion of [13C]linoleic acid and alpha-linolenic acid in
response tomarkedchanges in their dietary intake in men. J Lipid Res
2005, 46:269-280.
41. Sacks FM, Campos H: Polyunsaturated fatty acids, inflammation,
andcardiovascular disease: time to widen our view of the mechanisms.
J Clin Endocrinol Metab 2006, 91:398-400.
42. Willett WC: The role of dietary n-6 fatty acids in the prevention
ofcardiovascular disease. J Cardiovasc Med (Hagerstown) 2007, 8(Suppl
43. Dupasquier CMC, Weber AM, Ander BP, Rampersad PP, Steigerwald S,
Wigle JT, Mitchell RW, Kroeger EA, Gilchrist JSC, Moghadasian MM, Lukas
A, Pierce GN: The effects of dietary flaxseed on vascular contractile
function andatherosclerosis in rabbits during prolonged
hypercholesterolemia. Am J Physiol 2006, 291:H2987-H2996.
44. Dupasquier CMC, Dibrov E, Kneesh AL, Cheung PKM, Lee KGY, Alexander
HK, Yeganeh B, Moghadasian MH, Pierce GN: Dietary flaxseed
inhibitsatherosclerosis in the LDL receptor deficient mouse in part
through anti-proliferative andanti-inflammatory actions. Am J Physio l
2007, 293:H2394-2402.
45. Harris WS, Poston WC, Haddock CK: Tissue n-3 and n-6 fatty acids andrisk
for coronary heart disease events. Atherosclerosis 2007, 193:1-10.
46. Leren P: The Oslo diet-heart study. Eleven-year report. Circulation 1970,
47. Turpeinen O, Karvonen MJ, Pekkarinen M, et al.: Dietary prevention
ofcoronaryheart disease: the Finnish mental hospital study. Int J
Epidemiol 1979, 8:99-118.
48. Medical Research Council: Controlled trial of soya-bean oil
inmyocardialinfarction. Lancet 1968, 2:693-699.
49. Frantz ID, Dawson EA, Ashman PL, Gatewood LC, Bartsch GE, Kuba K,
Brewer ER: Test of effect of lipid lowering by diet on cardiovascular risk.
TheMinnesotacoronary sur vey. Arteriosclerosis 1989, 9:129-135.
50. Patenaude A, Rodriguez-Leyva D, Edel AL, Dibrov E, Dupasquier CM,
Austria JA, Richard MN, Chahine MN, Malcolmson LJ, Pierce GN:
Bioavailability of alpha-linolenic acid from flaxseed diets as a function
of the age of the subject. Eur J Clin Nutr 2009, 63:1123-9.
51. Austria JA, Richard MN, Chahine MN, Edel AL, Malcolmson LJ, Dupasquier
CM, Pierce GN: Bioavailability of alpha-linolenic acid in subjects
afteringestion of three different forms of flaxseed. J Am Coll Nutr 2008,
doi: 10.1186/1743-7075-7-32
Cite this article as: Rodriguez-Leyva and Pierce, The cardiac and haemo-
static effects of dietary hempseed Nutrition & Metabolism 2010, 7:32
... For centuries, hemp stems have been used for fibers (mats, shoes, cloth, and ropes) and its seeds have been used for oil production [7,9]. Moreover, hemp seeds are an excellent source of omega-3 and omega-6 fatty acids, as well as other nutritious oil and proteins [6,10,11]. Recently, the stem tissues desirable [28]. With the aim of simplifying the propagation method, eliminating the use of expensive bioreactors, using the lowest possible amount of nutrient media and hormones for rooting, and diminishing root disturbances, we attempted to combine the advantages of the older methods used by Tanaka et al. [39] and Nagae et al. [40], while also integrating recent applications such as those of Teixeira da Silva et al. [38], Kodym and Leeb [42], and Vidal and Sanchez [49]. ...
... Plants 2022,11, 1333 ...
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An alternative in vitro propagation protocol for medical Cannabis sativa L. cultivars for pharmaceutical industrial use was established. The aim of the protocol was to reduce the culture time, offering healthy and aseptic propagating material, while making the whole process more economic for industrial use. The propagation procedure was performed using plastic autoclavable vented and non-vented vessels, containing porous rooting fine-milled sphagnum peat moss-based sponges, impregnated in ½ Murashige and Skoog liquid growth medium, supplemented with indole-3-butyric acid (IBA) at various concentrations (0, 2.46, 4.92, and 9.84 µM) or by dipping nodal cuttings into 15 mM IBA aqueous solution. The highest average root numbers per cutting, 9.47 and 7.79 for high cannabidiol (H_CBD) and high cannabigerol (H_CBG) varieties, respectively, were achieved by dipping the cuttings into IBA aqueous solution for 4 min and then placing them in non-vented vessels. The maximum average root length in H_CBD (1.54 cm) and H_CBG (0.88 cm) was ascertained using 2.46 μM filter sterilized IBA in non-vented vessels. Filter-sterilized IBA at concentrations of 2.46 μM in vented and 4.92 μM in non-vented vessels displayed the maximum average rooting percentages in H_CBD (100%) and H_CBG (95.83%), respectively. In both varieties, maximum growth was obtained in non-vented vessels, when the medium was supplemented with 4.92 μM filter-sterilized IBA. Significant interactions between variety and vessel type and variety and IBA treatments were observed in relation to rooting traits. Approximately 95% of plantlets were successfully established and acclimatized in field. This culture system can be used not only for propagating plant material at an industrial scale but also to enhance the preservation and conservation of Cannabis genetic material.
... The ratio of omega-6 fatty acid linoleic acid (LA) to omega-3 fatty acid α-linolenic acid (ALA) found in hempseed is between 2:1 and 3:1, which is deemed ideal for a healthy diet. Much higher ratios, typically between 20:1 and 30:1, are recommended for patients with chronic diseases such as coronary artery disease, hypertension, diabetes, and cancer (Pierce and Rodriguez-Leyva 2010). According to Pierce and Rodriguez-Leyva (2010), hempseed has "almost as much protein as soybean and is also rich in Vitamin E and minerals. ...
... Much higher ratios, typically between 20:1 and 30:1, are recommended for patients with chronic diseases such as coronary artery disease, hypertension, diabetes, and cancer (Pierce and Rodriguez-Leyva 2010). According to Pierce and Rodriguez-Leyva (2010), hempseed has "almost as much protein as soybean and is also rich in Vitamin E and minerals. " Antunović et al. (2019) suggest that the nutritive properties of hemp seed and its derivatives (hemp seed oil, hemp seed cake) could also be used for animal feed "as a valuable source of crude protein and essential fat". ...
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Disease and foreign competition have made many growers question the viability of traditional commodity crops such as tomatoes, citrus, and avocados. Industrial hemp appears to be an attractive alternative, as the 2018 Farm Bill permitted its production. Florida has followed several other states in approving permits in 2020 to allow production of industrial hemp. Markets for industrial hemp products were promising in the first years of production, but market prices started to decline dramatically in 2020 with many growers unable to sell all their product. In this publication, we examine the hemp value chain with a focus on opportunities in the Florida market. Better coordination between regulators, processors, financial institutions, research and Extension services, and the retail sector to build confidence, harmonize policies, and lower transaction costs would help the industry flourish and ensure growers, consumers, and hemp-related enterprises can benefit from this emerging market.
... Due to the high nutritional value of hemp seed, it could be a complete food source for mankind. Each 100 g hemp seed provides 500-600 Kcal energy (Rodriguez-Leyva et al., 2010). Hemp seed usually contains 25-35% lipids, 20-25% proteins, and 20-30% carbohydrates as well as vitamins (thiamine, riboflavin, pyridoxine, vitamin E and C), minerals (mainly magnesium and iron), flavonoids, tocopherols (alpha, beta, gamma, and delta tocopherols), terpenes, phytosterols and bioactive peptides (Irakli et al., 2019). ...
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“Hemp” refers to non-intoxicating, low delta-9 tetrahydrocannabinol (Δ9-THC) cultivars of Cannabis sativa L. “Marijuana” refers to cultivars with high levels of Δ9-THC, the primary psychoactive cannabinoid found in the plant and a federally controlled substance used for both recreational and therapeutic purposes. Although marijuana and hemp belong to the same genus and species, they differ in terms of chemical and genetic composition, production practices, product uses, and regulatory status. Hemp seed and hemp seed oil have been shown to have valuable nutritional capacity. Cannabidiol (CBD), a non-intoxicating phytocannabinoid with a wide therapeutic index and acceptable side effect profile, has demonstrated high medicinal potential in some conditions. Several countries and states have facilitated the use of THC-dominant medical cannabis for certain conditions, while other countries continue to ban all forms of cannabis regardless of cannabinoid profile or low psychoactive potential. Today, differentiating between hemp and marijuana in the laboratory is no longer a difficult process. Certain thin layer chromatography (TLC) methods can rapidly screen for cannabinoids, and several gas and liquid chromatography techniques have been developed for precise quantification of phytocannabinoids in plant extracts and biological samples. Geographic regulations and testing guidelines for cannabis continue to evolve. As they are improved and clarified, we can better employ the appropriate applications of this uniquely versatile plant from an informed scientific perspective.
... Nrf-2 also blocks NF-κB-mediated upregulation of pro-inflammatory cytokines such as TNFα, IL-1β, and IL-6, and inflammatory events emanating from their accumulation or alone has been shown to prevent cholesterol accumulation and its ability to induce platelet aggregatory response, atherogenesis, and aortic contractility Gavel et al., 2011;Prociuk et al., 2006Prociuk et al., , 2008Richard et al., 2007). These protective mechanisms against cardiovascular degeneration were initially attributed to long-chain polyunsaturated fatty acids of hempseed (Rodriguez-Leyva & Pierce, 2010). However, current findings have established the contribution of HSPs and their peptides on the regulation of lipid metabolism and reduction in cardiovascular risks (Farinon et al., 2020;Lammi et al., 2019). ...
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Protein‐energy malnutrition is a global challenge that demands urgent attention, especially with the increasing population growth and unmatched food security plans. One strategy is to expand the list of protein sources, such as neglected and underutilized crops, with high protein content. A good number of plant proteins, in addition to their nutritional benefits, exert therapeutic properties as seen in seeds derived from legumes and emerging sources such as hemp. In this review, the transepithelial transport, functional, and biological properties of hempseed proteins (HSPs) and peptides were discussed. The review also described the potential safety issues of incorporating hempseeds in food products. Due to the multitargeted effects of hempseed‐derived proteins and their peptides against many chronic diseases, and their functional properties, current knowledge shows that hempseed has tremendous potential for functional food and nutraceutical applications. Practical applications The alarming rate of malnutrition and the attendant health consequences demand that underexploited nutrient‐rich crops should be incorporated as part of our common dietary sources. Among these crops, hempseed is gaining attention as an emerging source of proteins and peptides with promising potential in prevention and management of chronic diseases such as diabetes, hypertension, cancer, hypercholesterolemia, obesity, and diseases whose etiology involves oxidative stress and inflammation. Fortunately, a growing body of research evidence is demonstrating that hempseed is a reservoir of proteins and peptides with nutraceutical potentials for curbing life‐threatening diseases.
... In this category, flax (Linum usitatissimum L.) and camelina (Camelina sativa L.) are distinctive examples as the oils of their seeds are the richest sources of omega-3 fatty acid and α-linolenic acid (Crowley and Fröhlich 1998;Madhusudhan 2009). Additional examples of crops with high oilseed content include chia (Salvia hispanica L.), nigella (Nigella sativa L.), and hemp (Cannabis sativa L.) (Üstun et al. 1990;Rodriguez-Leyva and Pierce 2010;Mohd Ali et al. 2012). Both flax and industrial cannabis are also fiber crops with high-quality fibers widely used in textile and paper industries. ...
Climate change affects the sustainability of farming systems by downgrading soil fertility and diminishing crop yields. Agenda 2030 for Sustainable Development Goals aims to achieve key performance indicators to convert effectually currently degraded agroecosystems into smart, climate-resilient, and profitable farming systems. The introduction of alternative crops could equilibrate the negative impact of increased temperatures and water scarcity to ensure sufficient farm profitability. Alternative crops such as quinoa, teff, tritordeum, camelina, nigella, chia, and sweet potato show a high acclimatization potential to various conditions and could be components of novel re-designed agroecosystems, satisfying the goals the EU Green Deal for reduced chemical input use by 2030. In certain occasions, they adapt even better than conventional or traditional crops and could be integrated in crop rotations, demonstrating multiple uses that would benefit farmers. This review aimed to (i) evaluate seven alternative crops based on their potential contribution to climate change mitigation, in compliance with the EU (European Union) Green Deal objectives and the SDGs (Sustainable Development Goals) of the UN (United Nations), and (ii) examine the factors that would determine their successful integration in the Mediterranean Basin. These limiting factors for crop establishment included (i) soil properties (soil texture, pH value, salinity, and sodicity), (ii) environmental parameters (temperature, altitude, latitude, photoperiod), and (iii) crop performance and dynamics regarding water demands, fertilization needs, light, and heat requirements. All proposed crops were found to be adaptable to the Mediterranean climate characteristics and promising for the implementation of the goals of EU and UN.
The endocannabinoid system (ECS) was discovered in the early 1990s and is one of the most important neuroregulatory systems in the body. The ECS is responsible for homeostasis of most systems in the body. At a simplistic level, it is composed of endogenous ligands called endocannabinoids, cannabinoid receptors (CB1 and CB2 receptors), and enzymes that synthesize and degrade them. However, the ECS is actually more complex than this and there are other receptors and endocannabinoid-like substances involved in the ‘extended ECS’. CB1 receptors are particularly concentrated in the central nervous system and CB2 receptors are particularly concentrated in cells and tissues/organs of the immune system. However, cannabinoid receptors are also widely distributed throughout the body. In the nervous system, the classical understanding is that endocannabinoids are synthesized on demand in postsynaptic neurons and act as retrograde messengers, binding with cannabinoid receptors on presynaptic neurons to reduce neurotransmitter release from the presynaptic neuron. It is now known that there are also intracellular reservoirs and transporters of endocannabinoids. The ECS is critically involved in brain development, from the fetus through to adulthood. Dysfunction including deficiency of the ECS has been associated with a range of pathological disorders, including mental health conditions. The ECS plays a key role in the regulation of our mind and emotions and our reaction to stress. It is involved with the corticolimbic system and the HPA axis, both of which are key systems involved in regulation of stress and emotions. This chapter gives an overview of the ECS, as an understanding is necessary to later understand how medicinal cannabis may work in alleviating mental health disorders.
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Rising human population has increased the utilization of available resources for food, clothes, medicine, and living space, thus menacing natural environment and mounting the gap between available resources, and the skills to meet human desires is necessary. Humans are satisfying their desires by depleting available natural resources. Therefore, multifunctional plants can contribute towards the livelihoods of people, to execute their life requirements without degrading natural resources. Thus, research on multipurpose industrial crops should be of high interest among scientists. Hemp, or industrial hemp, is gaining research interest because of its fastest growth and utilization in commercial products including textile, paper, medicine, food, animal feed, paint, biofuel, biodegradable plastic, and construction material. High biomass production and ability to grow under versatile conditions make hemp, a good candidate species for remediation of polluted soils also. Present review highlights the morphology, adaptability, nutritional constituents, textile use, and medicinal significance of industrial hemp. Moreover, its usage in environmental conservation, building material, and biofuel production has also been discussed.
A direct link between hypercholesterolemia (HC) and renal pathologies has been established. Statins, the drugs of choice for HC management, have been associated with various side effects and toxicities, including nephropathy and other renal insults. Thus, natural dietary products based-alternative strategies for HC and associated pathologies are being considered. Objectives: Based on the unique nutritional composition and numerous health benefits of Hempseeds (Cannabis sativa), currently the potential anti-inflammatory and redox modulatory effects of hempseeds lipid extract (HEMP) against HC associated renal damage were evaluated and compared with statins (Simvastatin) in HFD induced experimental model of HC in rats. Design & Methods The hempseed lipid fractions (HEMP) were prepared and their ameliorating effects on HFD induced lipid profiles, renal function markers (RFT), histopathological/morphological changes, renal oxidative stress, and inflammation markers were studied and compared with statins (HFD+STATINS). Further, HEMP-mediated modulation of lipid metabolism mediators (APO B/E) was studied. Results: Not only, HEMP administration improved the lipid profiles and morphological signs of HC, but it also was safe compared to Simvastatin in terms of hepatic and renal function markers. Further, changes in renal histoarchitecture, biochemical markers of oxidative stress, and expression profiles of lipid metabolism and inflammatory pathways (Cox-1/2, PGDS, PGES) revealed that HEMP positively modulating the redox homeostasis activated the resolution pathways against HC associated renal insults. Conclusion: The outcomes of the current study indicated HEMP's ameliorative and therapeutic potential against hypercholesterolemia-associated nephropathies and other systemic effects.
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Delta9-Tetrahydrocannabinol (THC) is the main psychoactive compound present in marijuana. THC can also be found, as a contaminant, in some commercially available hemp products marketed in health food stores and on the internet as a good source of essential fatty acids. The products range from oil to alcoholic beverages to nutritional bars to candies, with oil being the most popular and commonly available. The analytical results are separated into two groups, products tested prior to and after publication of 21 CFR Part 1308, "clarification of listing of tetrahydrocannabinols." The data presented are a summary of 79 different hemp products tested for THC. THC was separated by a liquid-liquid or solid-liquid extraction, depending upon the product matrix. THC concentrations range from none detected to 117.5 microg THC/g material. Typical limits of detection for the assay (depending on matrix) are 1.0-2.5 microg THC/g material. Products that were of aqueous base (beer, tea) had much lower limits of detection (2.5 ng/mL). No THC was detected in 58% of the products from group 1 and 86% of the products from group 2. The amounts indicate that THC levels in currently marketed hemp products are significantly lower than in those products available before 2003 and reported in previous studies. The results reported here may be used as a general guideline for the THC content of hemp products recently found in the marketplace today.
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Hempseed is a novel functional food that contains several health-promoting polyunsaturated fatty acids (PUFAs). PUFAs, such as those found in flaxseed and fish, have been shown to protect the heart against arrhythmias following ischemia/reperfusion. TO INVESTIGATE THE POTENTIAL OF DIETARY HEMPSEED AS A CARDIOPROTECTIVE AGENT AGAINST GLOBAL ISCHEMIA AND SUBSEQUENT REPERFUSION BY ASSESSING SEVERAL MEASUREMENTS OF CARDIAC PERFORMANCE: QT interval duration, left ventricular pressure, arrhythmia incidence and arrhythmia duration. MALE NEW ZEALAND WHITE RABBITS WERE FED ONE OF SIX DIETS: a control diet; or one supplemented with 10% hempseed, 10% delipidated hempseed, 0.5% cholesterol, 0.5% cholesterol plus 10% hempseed or 5% coconut oil. After eight weeks on their respective diets, the hearts were excised and subjected to 30 min of global ischemia and 45 min of reperfusion. Electrocardiogram traces were recorded throughout the experiment and were subsequently analyzed for QT interval duration, left ventricular pressure, arrhythmia incidence and arrhythmia duration. Plasma and cardiac tissue were analyzed for fatty acid content and composition. Cholesterol-fed animals exhibited significantly higher PUFA levels in their plasma, but this did not directly translate into higher PUFA levels in their cardiac fractions. There were no significant differences among the groups in the incidence or duration of ischemia-derived arrhythmias. During reperfusion, there was a significant decrease in the incidence of fibrillation in the hearts obtained from cholesterol-fed and hempseed- plus cholesterol-fed rabbits compared with the hearts from delipidated hempseed-fed rabbits. Dietary hempseed induced limited beneficial effects on cardiac function during ischemia/reperfusion challenge. The present study does not support the use of dietary hempseed to protect the heart during ischemic insult in this experimental model.
We studied the long-chain conversion of [U-C-13](alpha-linolenic acid (ALA) and linoleic acid (ALA) and responses of erythrocyte phospholipid composition to variation in the dietary ratios of 18:3n-3 (ALA) and 18:2n-6 (LA) for 12 weeks in 38 moderately hyperlipidemic men. Diets were enriched with either flaxseed oil (FXO; 17 g/day ALA, n = 2 1) or sunflower oil (SO; 17 g/day LA, n = 17). The FXO diet induced increases in phospholipid ALA ( > 3-fold), 20:5n-3 [eicosapentaenoic acid (EPA), > 2-fold], and 22:5n-3 [docosapentaenoic acid (DPA), 50%] but no change in 22:6n-3 [docosahexanoic acid (DHA)], LA, or 20:4n-6 [arachidonic acid (AA)]. The increases in EPA and DPA but not DHA were similar to those in subjects given the SO diet enriched with 3 g of EPA plus DHA from fish oil (n = 19). The SO diet induced a small increase in LA but no change in AA. Long-chain conversion of [U-13C]AILA and [U-13C]LA, calculated from peak plasma C-13 concentrations after simple modeling for tracer dilution in subsets from the FXO (n = 6) and SO (n = 5) diets, was similar but low for the two tracers (i.e., AA, 0.2%; EPA, 0.3%; and DPA, 0.02%) and varied directly with precursor concentrations and inversely with concentrations of fatty acids of the alternative series.jlr [C-13]DHA formation was very low ( < 0.01 %) with no dietary influences.-Hussein, N., E. Ah-Sing, P. Wilkinson, C. Leach, B. A. Griffin I, and D.J. Millward. Long-chain conversion of [C-13]linoleic acid and alpha-linolenic acid in response to marked changes in their dietary intake in men.
The seed of Cannabis sativa L. has been an important source of nutrition for thousands of years in Old World cultures. Non-drug varieties of Cannabis, commonly referred to as hemp, have not been studied extensively for their nutritional potential in recent years, nor has hempseed been utilized to any great extent by the industrial processes and food markets that have developed during the 20th century. Technically a nut, hempseed typically contains over 30% oil and about 25% protein, with considerable amounts of dietary fiber, vitamins and minerals. Hempseed oil is over 80% in polyunsaturated fatty acids (PUFAs), and is an exceptionally rich source of the two essential fatty acids (EFAs) linoleic acid (18:2 omega-6) and alpha-linolenic acid (18:3 omega-3). The omega-6 to omega-3 ratio (n6/n3) in hempseed oil is normally between 2:1 and 3:1, which is considered to be optimal for human health. In addition, the biological metabolites of the two EFAs, gamma-linolenic acid (18:3 omega-6; GLA) and stearidonic acid (18:4 omega-3; SDA), are also present in hempseed oil. The two main proteins in hempseed are edestin and albumin. Both of these high-quality storage proteins are easily digested and contain nutritionally significant amounts of all essential amino acids. In addition, hempseed has exceptionally high levels of the amino acid arginine. Hempseed has been used to treat various disorders for thousands of years in traditional oriental medicine. Recent clinical trials have identified hempseed oil as a functional food, and animal feeding studies demonstrate the long-standing utility of hempseed as an important food resource.
Gamma linolenic acid (GLA) is a member of the n-6 family of polyunsaturated fatty acids and can be synthesized from linoleic acid (LA) by the enzyme delta-6-desaturase. The therapeutic values of GLA supplementation have been documented, but the molecular mechanism behind the action of GLA in health benefits is not clear. In this study, we assessed the effect of GLA with that of LA on lipopolysaccharide (LPS)-induced inflammatory responses and further explored the molecular mechanism underlying the pharmacological properties of GLA in mouse RAW 264.7 macrophages. GLA significantly inhibited LPS-induced protein expression of inducible nitric oxide synthase, pro-interleukin-1beta, and cyclooxygenase-2 as well as nitric oxide production and the intracellular glutathione level. LA was less potent than GLA in inhibiting LPS-induced inflammatory mediators. Both GLA and LA treatments dramatically inhibited LPS-induced IkappaB-alpha degradation, IkappaB-alpha phosphorylation, and nuclear p65 protein expression. Moreover, LPS-induced nuclear factor-kappaB (NF-kappaB) and activator protein-1 (AP-1) nuclear protein-DNA binding affinity and reporter gene activity were significantly decreased by LA and GLA. Exogenous addition of GLA but not LA significantly reduced LPS-induced expression of phosphorylated extracellular signal-regulated kinase (ERK) 1/2 and c-Jun N-terminal kinase (JNK)-1. Our data suggest that GLA inhibits inflammatory responses through inactivation of NF-kappaB and AP-1 by suppressed oxidative stress and signal transduction pathway of ERK and JNK in LPS-induced RAW 264.7 macrophages.