Accurate and simple sizing of primer extension products using a non-radioactive approach facilitates identification of transcription initiation sites

ArticleinJournal of microbiological methods 81(3):256-8 · April 2010with13 Reads
Impact Factor: 2.03 · DOI: 10.1016/j.mimet.2010.03.021 · Source: PubMed


    An improved method of non-radioactive identification of transcription start sites is presented in which the use of 7-deaza dGTP in the primer extension reaction allows the product to be directly aligned to cycle sequencing traces on an automated sequencer. This removes the documented need to apply corrections for mobility differences.