Mapping of the interaction sites between Wee1 kinase and the regulatory ß-subunit of protein kinase CK2
Institute of Biochemistry and Molecular Biology, University of Southern Denmark, 5230 Odense, Denmark.International Journal of Oncology (Impact Factor: 3.03). 05/2010; 36(5):1175-82. DOI: 10.3892/ijo-00000600
Somatic Wee1 is a protein kinase that plays a key role in cell cycle progression at the onset of mitosis by phosphorylating CDK1 at the inhibitory Tyr15 amino acid residue. Wee1 is regulated at multiple levels, i.e., phosphorylation, protein-protein association and proteasome-mediated degradation. We have recently shown that the regulatory beta-subunit of protein kinase CK2 participates in PLK1-Wee1 complex formation interacting directly with Wee1 and thereby contributing to the regulation of G2-M cell cycle transition. Here, we show that Wee1 binds CK2beta via two domains comprising amino acids 59-71 and 232-332. By employing deletion mutants of the CK2beta-subunit we also show that two regions between residues 1-5 and 155-170 are necessary for binding Wee1. Furthermore, we demonstrate that the interaction between CK2beta and Wee1 does not modify the kinase activity of the latter, instead CK2beta directly upregulates CDK1 kinase activity by reversing the inhibitory effect which follows Wee1-mediated phosphorylation. Taken together, our findings reinforce the notion that CK2beta is a modulator of protein kinases implicated in cell cycle regulation and exerts functions that are independent of CK2 tetramers.
- [Show abstract] [Hide abstract]
ABSTRACT: From the Outside to the Inside: Interaction of CK2 with Membrane Proteins Regulating Gene Expression: Interaction of CK2 with Components of Signaling Cascades, Transcription Factors and DNA Modifying Enzymes Mastering Nucleic Acid Functions: Interaction of CK2 with Proteins of the Replication, Transcription, and Translation Machinery of the Cell Let It Roll: Interaction of CK2 with Cell Cycle Regulatory Proteins Guardian Angels: Interaction of CK2 with Proteins That Maintain the Cellular Integrity Live and Let Die: Interaction of CK2 with Proteins of the Apoptotic Pathway Highways in the Cell: Interaction of CK2 with the Cytoskeleton and Motor Proteins Collaborating with the Enemy: Interaction of CK2 with Proteins Implicated in Viral Infections Last But Not Least: Miscellaneous
- [Show abstract] [Hide abstract]
ABSTRACT: Screening for protein kinase CK2 inhibitors of the structural diversity compound library (DTP NCI/NIH) led to the discovery of 4-[(E)-(fluoren-9-ylidenehydrazinylidene)-methyl]benzoic acid (E9). E9 induces apoptotic cell death in various cancer cell lines and upon hypoxia, the compound suppresses CK2-catalyzed HSP90/Cdc37 phosphorylation and induces HIF-1α degradation. Furthermore, E9 exerts a strong anti-tumour activity by inducing necrosis in murine xenograft models underlining its potential to be used for cancer treatment in future clinical studies. Crystal structure analysis of human and maize CK2α in complex with E9 reveals unique binding properties of the inhibitor to the enzyme, accounting for its affinity and selectivity. Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.