In Vivo Evidence of the Immunomodulatory Activity of Orally Administered Aloe vera Gel

College of Pharmacy, Chungbuk National University, Cheongju, 361-763, Korea.
Archives of Pharmacal Research (Impact Factor: 2.05). 03/2010; 33(3):451-6. DOI: 10.1007/s12272-010-0315-1
Source: PubMed


The gels of Aloe species contain immunomodulatory components such as aloctin A and acemannan. Most studies on these gels were performed in in vitro cell culture systems. Although several studies examined their immunomodulatory activity in vivo, the route of administration was intraperitoneal or intramuscular. Here, we evaluated the in vivo immunomodulatory activity of processed Aloe vera gel (PAG) in mice. Oral administration of PAG significantly reduced the growth of C. albicans in the spleen and kidney following intravenous injection of C. albicans in normal mice. PAG administration also reduced the growth of C. albicans in streptozotocin-induced diabetic mice. PAG administration did not increase ovalbumin (OVA)-specific cytotoxic T lymphocyte (CTL) generation in normal mice, but did increase it in high-fat-diet induced diabetic mice. These findings provide the first clear evidence for the immunomodulatory activity of orally administered Aloe vera gel.

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Available from: Young In Park, Aug 13, 2014
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    • "Many investigators have shown that Aloe vera extract induces hepatoprotective effects (Arosio et al., 2000; Chandan et al., 2007); protects against heavy metals induce oxidative stress (Gupta & Flora, 2005); as well as enhances anti-inflammatory properties (Bezakova, Oblozinsky, Sykorova, Paulikova, & Kostalova, 2005; Wu, 2008). On the other hand, aloe vera accelerates protection and healing effects against ulcerative colitis (Korkina et al., 2003); promotes antioxidant status (Saada, Ussama, & Mahdy, 2003; Lim et al., 2003) and induces immune-stimulation actions (Hart et al., 1990; Im et al., 2010; Peng et al., 1991). "
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    ABSTRACT: The current study was conducted to evaluate the modulating efficacy of prolonged administration of Aloe vera extract against γ-irradiation-induced oxidative stress in male rats. Aloe vera extract was orally administrated in a dose of 300 mg/kg body weight daily for 21 successive days before irradiation (5 Gy as an acute dose) and 7 days post exposure. The oxidative stress markers; malondialdehyde (MDA), reduced glutathione (GSH) and metallothionein (MT) were estimated in hepatic tissues. Levels of certain trace metals namely iron, copper and zinc were determined in blood, liver and intestinal tissues as well as in Aloe vera extract, in addition histological examination of liver tissues was investigated. Rats exposed to ionizing radiation had elevation in MDA, depletion in GSH and MT level, in addition to alterations in trace metal concentration in blood, liver and intestine. Photomicrograph showed abnormalities in hepatic tissues. Aloe vera extract administration pre- and post-γ-irradiation resulted in noticeable improvement in the studied antioxidant status of liver tissue; restored the control levels of Fe and Cu levels in liver and intestine as well as intestinal Zn. In conclusion, Aloe vera extract have a beneficial protective effect against radiation-induced oxidative stress.
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    • "The beneficial properties of the plant have been ascribed to the inner, colorless leaf gel that can function both orally and topically as an active immunomodulator. The gel of Aloe vera has been reported to stimulate wound-healing and skin hydration, induce hematopoiesis, and possess anti-diabetic, anti-carcinogenic, antimicrobial, anti-oxidant as well as anti-inflammatory activities (Dat et al., 2012; Hamman, 2008a; Im et al., 2010; Surjushe et al., 2008; Vazquez et al., 1996). Over 75 active components have already been identified in Aloe vera leaf gels (Hamman, 2008b), and some of them have been implicated as immunomodulatory compounds based on animal studies. "
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    ABSTRACT: Aloe vera has been used in traditional herbal medicine as an immunomodulatory agent inducing anti-inflammatory effects. However, its role on the IL-1β inflammatory cytokine production has not been studied. IL-1β production is strictly regulated both at transcriptional and posttranslational levels through the activity of Nlrp3 inflammasome. In this study we aimed to determine the effect of Aloe vera on the molecular mechanisms of Nlrp3 inflammasome-mediated IL-1β production in LPS-activated human THP-1 cells and monocyte-derived macrophages. Our results show that Aloe vera significantly reduced IL-8, TNFα, IL-6 and IL-1β cytokine production in a dose dependent manner. The inhibitory effect was substantially more pronounced in the primary cells. We found that Aloe vera inhibited the expression of pro-IL-1β, Nlrp3, caspase-1 as well as that of the P2X7 receptor in the LPS-induced primary macrophages. Furthermore, LPS-induced activation of signaling pathways like NF-κB, p38, JNK and ERK were inhibited by Aloe vera in these cells. Altogether, we show for the first time that Aloe vera-mediated strong reduction of IL-1β appears to be the consequence of the reduced expression of both pro-IL-1β as well as Nlrp3 inflammasome components via suppressing specific signal transduction pathways. Furthermore, we show that the expression of the ATP sensor P2X7 receptor is also downregulated by Aloe vera that could also contribute to the attenuated IL-1β cytokine secretion. These results may provide a new therapeutic approach to regulate inflammasome-mediated responses.
    Full-text · Article · Jul 2013 · Molecular Immunology
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    • "This anti-inflammatory effect was detectable in the A.V.-treated group from three days after treatment. The anti-inflammatory properties of A.V. and its efficacy in some other diseases have been shown in several previously conducted studies.[211–131718] Some well-known antioxidant constituents in A.V. gel (e.g., flavonoids, saponins, sterols, terpenoids…) might contribute to its anti-inflammatory properties.[101419] "
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    ABSTRACT: Aphthous ulcer is one of the most common diseases of the oral cavity with no known effective treatment so far, which could cause severe discomfort in patients. Aloe vera (A.V.) is a tropical plant with anti-inflammatory and immunostimulant effects, which could be of benefit in a diversity of wound healing conditions. The aim of this study is to evaluate topically administered A.V. gel on oral cavity minor aphthous healing. As a double-blind (case control) clinical trial, 40 patients with oral minor aphthous lesions were randomly allocated in either the case group (A.V. gel) or the control (placebo) group. The healing time (days after gel application), patient's pain score; the lesion and its surrounding inflammation diameters were recorded for 2 weeks. The obtained results were analyzed by either "Fishers exact" or t-student test using SPSS software. The mean (±SD) of patients' age was 29.25 ± 8.48 and 27.95 ± 7.96 years in the control and A.V.-treated groups, respectively, which were not significantly different (P > 0.05). The duration of complete wound healing, pain score, wound size and inflammation zone diameter in the A.V.-treated group were significantly lower than the control group (P ≤ 0.05) on specific time points after treatment. It seems likely that A.V. 2% oral gel is not only effective in decreasing the recurrent aphthous stomatitis patients' pain score and wound size but also decreases the aphthous wound healing period.
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