Systematic Analysis of Human Protein Complexes Identifies Chromosome Segregation Proteins

Research Institute of Molecular Pathology (IMP), Dr. Bohr-Gasse 7, A-1030 Vienna, Austria.
Science (Impact Factor: 33.61). 04/2010; 328(5978):593-9. DOI: 10.1126/science.1181348
Source: PubMed


Chromosome segregation and cell division are essential, highly ordered processes that depend on numerous protein complexes.
Results from recent RNA interference screens indicate that the identity and composition of these protein complexes is incompletely
understood. Using gene tagging on bacterial artificial chromosomes, protein localization, and tandem-affinity purification–mass
spectrometry, the MitoCheck consortium has analyzed about 100 human protein complexes, many of which had not or had only incompletely
been characterized. This work has led to the discovery of previously unknown, evolutionarily conserved subunits of the anaphase-promoting
complex and the γ-tubulin ring complex—large complexes that are essential for spindle assembly and chromosome segregation.
The approaches we describe here are generally applicable to high-throughput follow-up analyses of phenotypic screens in mammalian

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Available from: Andrei I. Pozniakovsky
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