The chondroprotective effects of ferulic acid on hydrogen peroxide-stimulated chondrocytes: Inhibition of hydrogen peroxide-induced pro-inflammatory cytokines and metalloproteinase gene expression at the mRNA level

ArticleinAgents and Actions 59(8):587-95 · March 2010with68 Reads
DOI: 10.1007/s00011-010-0165-9 · Source: PubMed
The objective of the study is to evaluate the effect of ferulic acid (FA), an antioxidant from the Chinese herb Dong-Gui [Chinese angelica, Angelica sinensis (Oliv.) Diels], on the regulation of various genes in hydrogen peroxide-stimulated porcine chondrocytes at the mRNA level. The effect of FA and the effective concentration of FA on porcine chondrocytes was evaluated by the lactate dehydrogenase, WST-1, crystal violet assay, and a chemical luminescence assay. Gene expression in hydrogen peroxide-stimulated chondrocytes either pre- or post-treated with FA was evaluated by real-time PCR. Chondrocytes pre-treated with 40 microM FA decreased the hydrogen peroxide-induced interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), and MMP-1 and partially restored SOX9 gene expression. Post-treatment with 40 microM FA also decreased the expression of MMP-1 and MMP-13. FA decreased the hydrogen peroxide-induced IL-1beta, TNF-alpha, MMP-1 and MMP-13 and increased SOX9 gene expression. These findings suggest that FA may prove to be important in the treatment of osteoarthritis. Further research is needed.
    • "In the present study, HPLC profile of MU has shown the presence of four main polyphenolic acids (gallic acid, vanillic acid, p-coumaric acid, and ferulic acid) and three major flavonoids (catechin, morin, and quercetin) which have been reported to have multiple effects such as anti-inflammatory, anti-oxidative, and immunomodulatory properties. Several studies have reported that ferulic acid possesses anti-inflammatory effect by suppressing the expression of proinflammatory cytokines (TNF-a and IL-1b), matrix metalloproteinases , and inflammatory enzymes (COX-2 and iNOS) through the inhibition of NF-jB pathway in various inflammatory diseases models [36] . In addition, gallic acid has been reported to have antiarthritic effect by inducing the apoptosis of rheumatoid arthritis (RA) fibroblast-like synoviocytes (FLS) by regulating the expression of apoptosis-related proteins and reducing the expression of pro-inflammatory mediators [37] . "
    [Show abstract] [Hide abstract] ABSTRACT: The present study was aimed to investigate the anti-arthritic effect of majoon ushba (MU) and its underlying mechanism in adjuvant induced arthritis (AIA) rats. Arthritis was induced by intradermal injection of complete freund's adjuvant (0.1ml) into the right hind paw of the Wistar albino rats. MU (1000mg/kg/b.wt) and methotrexate (3mg/kg/b.wt) were administered from day 11 to day 18th for 8days after adjuvant induction. We have found that MU treatment significantly increased the level of anti-inflammatory cytokine (IL-10) and inhibited the over production of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-6) and monocyte chemoattractant protein-1 (MCP-1) (ELISA) in the serum of adjuvant-induced arthritic rats. The mRNA expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-17), inflammatory enzymes (inducible nitric oxide synthase (iNOS) and cyclo-oxygenase-2 (COX-2)), MCP-1, receptor activator of nuclear factor-kB ligand (RANKL) and transcription factors (NF-кB and AP-1) (Real-Time PCR) was found significantly downregulated in the synovial tissues of MU treated arthritic rats. In addition, the protein expression of NF-кB, IL-17, COX-2, and RANKL (western blotting and immunohistochemistry analysis) was found reduced. On the other hand, osteoprotegerin (OPG), a bone remodeling marker was found to be elevated in synovial tissues of MU treated arthritic rats. Furthermore, MU treatment prevented body weight loss and reduced the joint paw edema, cell infiltration, cartilage and bone degradation as evidenced by the histopathological and radiological analysis. In conclusion, our current findings provide scientific evidence for the traditional claim of MU as an anti-arthritic drug.
    Article · Nov 2015
    • "Thus, the levels of TNF-í µí»¼ and IL-1í µí»½ in plasma may reflect the activity of inflammatory cells [25]. A previous report has indicated that FA had the ability to decrease the levels of hydrogen peroxide-induced IL-1í µí»½, TNF-í µí»¼, MMP-1, and MMP-13, thereby reducing bone destruction [26], synovitis, and the erosion of cartilage in antigen-induced arthritis [27]. Because of their purported protective effects on joints, FA and OD (which contains FA) were administered to CIA rats, and, as shown in Figures 4–10, the animals' symptoms ware ameliorated, the number of inflammatory cells decreased, and the levels of IL-1í µí»½ and TNF-í µí»¼ declined by day 28 and day 42. "
    [Show abstract] [Hide abstract] ABSTRACT: Objectives. This study aimed to identify the active compounds in Oldenlandia diffusa (OD) decoction and the compounds absorbed into plasma, and to determine whether the absorbed compounds derived from OD exerted any anti-inflammatory effects in rats with collagen induced arthritis (CIA). Methods. The UPLC-PDA (Ultra Performance Liquid Chromatography Photo-Diode Array) method was applied to identify the active compounds both in the decoction and rat plasma. The absorbable compound was administered to the CIA rats, and the effects were dynamically observed. X-ray films of the joints and HE stain of synovial tissues were analyzed. The levels of IL-1β and TNF-α in the rats from each group were measured by means of ELISA. The absorbed compound in the plasma of CIA rats was identified as ferulic acid (FA), following OD decoction administration. Two weeks after the administration of FA solution or OD decoction, the general conditions improved compared to the model group. The anti-inflammatory effect of FA was inferior to that of the OD decoction ( P < 0.05 ), based on a comparison of IL-1β TNF-α levels. FA from the OD decoction was absorbed into the body of CIA rats, where it elicited anti-inflammatory responses in rats with CIA. Conclusions. These results suggest that FA is the bioactive compound in OD decoction, and FA exerts its effects through anti-inflammatory pathways.
    Full-text · Article · May 2014
    • "However, they are the secondary symptoms subsequent to the primary cause, possibly inflammation and oxidative stress. Some studies have explored herbal extracts and their components for treating osteoarthritis by alleviating inflammation (Bar-Yehuda et al., 2009; Chen et al., 2010). Several studies have investigated anti-osteoarthritis effects of rose hip in human study, and the effect has been measured only by the degree of pain relief in patients with osteoarthritis, but they have not investigated its mechanism (Warholm et al., 2003; Winther et al., 2005). "
    [Show abstract] [Hide abstract] ABSTRACT: Although rose hip administration reduces pain and stiffness in patients with osteoarthritis, the mechanism of rose hip to alleviate primary symptoms has not been investigated. We examined how two types of Rosa canina, grown in Denmark (I-Flex) and Coesam in Chile, attenuate the osteoarthritis symptoms in male rats with osteoarthritis. Therefore pain-related behaviors were evaluated, and histological changes and cytokine expression in the articular cartilage of right knee induced osteoarthritis were assessed via intra-articular monoiodoacetate (MIA) injection. The right knee induced swollen knee, limping legs, and disproportional weight distribution into the right hind paw, and reduced maximum velocity to run on a treadmill from day 3 after MIA injection. The symptoms were exacerbated up to about 2 weeks and remained steady until day 21. Overall the osteoarthritis symptoms in MIA-injected control rats did not significantly change over the experimental period. After 21 days, bone mineral density in right leg and knee had greatly decreased in rats injected with MIA. These symptoms were related to increased expression of matrix metalloprotinase (MMP)-3 and MMP-13 in articular cartilage that degraded collage and elevated the production of TNF-α, IL-1β, and IL-6. However, both types of rose hip markedly attenuated all of the symptoms experienced by the control and overall symptoms from day 3 were improved at day 21. However, some symptoms of osteoarthrit remained and there was no difference between both rose hips for alleviating osteoarthritis symptoms. In conclusion, rose hips from Denmark and Coesam in Chile are potential therapeutic agents for the protection of articular cartilage against progression of osteoarthritis.
    Full-text · Article · Apr 2014
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