Conference Paper

Detection of Ralstonia Pickettii Bacteria in M9 Medium using Polymer Nanocomposite Sensors

Univ. of Limerick, Limerick
DOI: 10.1109/SAS.2007.374360 Conference: Sensors Applications Symposium, 2007. SAS '07. IEEE
Source: IEEE Xplore

ABSTRACT

Detection of bacteria such as Ralstonia pickettii in high purity water (HPW) has gained increasing interest for researchers. Contamination problems in HPW in both pharmaceutical and semiconductor applications can lead to a reduction in performance and inefficiency in production chains leading to huge expense. Real time detection of these bacteria in HPW prior to use can allow corrective action to be taken sooner, by reallocating the water for repurification or utilization elsewhere thereby reducing industrial costs The aim of this study was to evaluate the responses of polymer nanocomposite sensors to increasing concentrations of Ralstonia pickettii bacteria. M9 minimal growth medium was used to grow the bacteria and three different concentrations were prepared by taking samples after 2 hours, 4 hours and 6 hours of growth. The sensors showed large sensitivity to each concentration of bacteria and produced a decrease in response over the growth cycle of the bacteria. These sensors demonstrated the ability to detect early stage actively growing cells in real time, which is of large benefit to HPW applications. This work has shown that these sensors can be used to detect both lag phase and late exponential and stationary phase growing cells in a minimal nutrient environment.

2 Followers
 · 
5 Reads
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The research and development of new electronic-nose applications in the biomedical field has accelerated at a phenomenal rate over the past 25 years. Many innovative e-nose technologies have provided solutions and applications to a wide variety of complex biomedical and healthcare problems. The purposes of this review are to present a comprehensive analysis of past and recent biomedical research findings and developments of electronic-nose sensor technologies, and to identify current and future potential e-nose applications that will continue to advance the effectiveness and efficiency of biomedical treatments and healthcare services for many years. An abundance of electronic-nose applications has been developed for a variety of healthcare sectors including diagnostics, immunology, pathology, patient recovery, pharmacology, physical therapy, physiology, preventative medicine, remote healthcare, and wound and graft healing. Specific biomedical e-nose applications range from uses in biochemical testing, blood-compatibility evaluations, disease diagnoses, and drug delivery to monitoring of metabolic levels, organ dysfunctions, and patient conditions through telemedicine. This paper summarizes the major electronic-nose technologies developed for healthcare and biomedical applications since the late 1980s when electronic aroma detection technologies were first recognized to be potentially useful in providing effective solutions to problems in the healthcare industry.
    Full-text · Article · Dec 2011 · Sensors
  • [Show abstract] [Hide abstract]
    ABSTRACT: Clean water is one of our most valuable natural resources. In addition to providing safe drinking water it assures functional ecosystems that support fisheries and recreation. Human population growth and its associated increased demands on water pose risks to maintaining acceptable water quality. It is vital to assess source waters and the aquatic systems that receive inputs from industrial waste and sewage treatment plants, storm water systems, and runoff from urban and agricultural lands. Rapid and confident assessments of aquatic resources form the basis for sound environmental management. Current methods engaged in tracing the presence of various bacteria in water employ bulky laboratory equipment and are time consuming. Thus, real-time water quality monitoring is essential for National and International Health and Safety. Environmental water monitoring includes measurements of physical characteristics (e.g. pH, temperature, conductivity), chemical parameters (e.g. oxygen, alkalinity, nitrogen and phosphorus compounds), and abundance of certain biological taxa. Monitoring could also include assays of biological activity such as alkaline phosphatase, tests for toxins such as microcystins and direct measurements of pollutants such as heavy metals or hydrocarbons. Real time detection can significantly reduce the level of damage and also the cost to remedy the problem. This paper presents overview of state-of-the-art methods and devices used for point-of-access water quality monitoring and suggest further developments in this area.
    No preview · Chapter · Jan 2012
  • [Show abstract] [Hide abstract]
    ABSTRACT: This paper presents the design of a novel receiver for a broadband ultrasonic Local Positioning System (LPS). As in other devices based on matched filtering of the transmitted encoded signals, this receiver features high robustness to noise and multiple access capability. Unlike previous solutions, the proposed receiver is capable of detecting beacons emissions despite being installed in a fast moving device, and it also provides estimation of its velocity. The system has been implemented in an FPGA-based architecture. An experimental analysis of performance has been carried out. Firstly using a set of test signals synthetically generated to simulate different positions and velocities of the receiver. Secondly, a set of real signals obtained with a prototype have been used. The simulated results show the capability of the system to detect the encoded signals emitted by the LPS infrastructure when the device is moving at velocities of up to 3 m/s under low SNR conditions. The real results confirm the improved system capability, but also make evident the negative influence that phenomena such as the transducers response and multipath propagation can have on system performance.
    No preview · Article · Jan 2013 · Sensors and Actuators A Physical