Estrogenic and AhR activities in dissolved phase and suspended solids from wastewater treatment plants

ArticleinScience of The Total Environment 408(12):2608-15 · March 2010with15 Reads
Impact Factor: 4.10 · DOI: 10.1016/j.scitotenv.2010.02.034 · Source: PubMed
Abstract

The distribution of estrogen receptor (ERalpha) and Aryl Hydrocarbon Receptor (AhR) activities between the dissolved phase and suspended solids were investigated during wastewater treatment. Three wastewater treatment plants with different treatment technologies (waste stabilization ponds (WSPs), trickling filters (TFs) and activated sludge supplemented with a biofilter system (ASB)) were sampled. Estrogenic and AhR activities were detected in both phases in influents and effluents. Estrogenic and AhR activities in wastewater influents ranged from 41.8 to 79 ng/L E(2) Eq. and from 37.9 to 115.5 ng/L TCDD Eq. in the dissolved phase and from 5.5 to 88.6 ng/g E(2) Eq. and from 15 to 700 ng/g TCDD Eq. in the suspended solids. For both activities, WSP showed greater or similar removal efficiency than ASB and both were much more efficient than TF which had the lowest removal efficiency. Moreover, our data indicate that the efficiency of removal of ER and AhR activities from the suspended solid phase was mainly due to removal of suspended solids. Indeed, ER and AhR activities were detected in the effluent suspended solid phase indicating that suspended solids, which are usually not considered in these types of studies, contribute to environmental contamination by endocrine disrupting compounds and should therefore be routinely assessed for a better estimation of the ER and AhR activities released in the environment.

    • "Another study compared water samples taken upstream and downstream of seven WWTPs, six of which showed greater concentrations of TEQbio downstream of the effluents than upstream (Jaro sov a et al., 2012). Two additional studies reported the concentration in a single sample in China to be < 0.01 ng TEQbio/L (Ma et al., 2005) and three samples from France ranging from 37 to 115 ng TEQbio/L (Dagnino et al., 2010). "
    [Show abstract] [Hide abstract] ABSTRACT: Discharge of substances like pesticides, pharmaceuticals, flame retardants, and chelating agents in surface waters has increased over the last decades due to the rising numbers of chemicals used by humans and because many WWTPs do not eliminate these substances entirely. The study, results of which are presented here, focused on associations of (1) concentrations of micropollutants in wastewater treatment plant (WWTP) effluents, surface waters, sediments, and tissues of fishes; (2) results of laboratory biotests indicating potentials for effects in these samples and (3) effects either in feral chub (Leuciscus cephalus) from two German rivers (Schussen, Argen) or in brown trout (Salmo trutta f. fario) and rainbow trout (Oncorhynchus mykiss) exposed in bypass systems to streamwater of these rivers or in cages directly in the rivers. The Schussen and Argen Rivers flow into Lake Constance. The Schussen River is polluted by a great number of chemicals, while the Argen River is less influenced by micropollutants. Pesticides, chelating agents, flame retardants, pharmaceuticals, heavy metals, polychlorinated biphenyls (PCBs), and polybrominated diphenyl ethers (PBDEs) were detected in effluents of a WWTP discharging to the Schussen as well as in surface water, and/or fishes from downstream of the WWTP. Results obtained by biotests conducted in the laboratory (genotoxicity, dioxin-like toxicity, and embryotoxicity) were linked to effects in feral fish collected in the vicinity of the WWTP or in fishes exposed in cages or at the bypass systems downstream of the WWTP. Dioxin-like effect potentials detected by reporter gene assays were associated with activation of CYP1A1 enzymes in fishes which are inducible by dioxin-like chemicals. Abundances of several PCBs in tissues of fishes from cages and bypass systems were not associated with these effects but other factors can influence EROD activity. Genotoxic potentials obtained by in vitro tests were associated with the presence of micronuclei in erythrocytes of chub from the river. Chemicals potentially responsible for effects on DNA were identified. Embryotoxic effects on zebrafish (Danio rerio), investigated in the laboratory, were associated with embryotoxic effects in trout exposed in streamwater bypass systems at the two rivers. In general, responses at all levels of organization were more pronounced in samples from the Schussen than in those from the Argen. These results are consistent with the magnitudes of chemical pollution in these two streams. Plausibility chains to establish causality between exposures and effects and to predict effects in biota in the river from studies in the laboratory are discussed.
    Full-text · Article · Sep 2014 · Water Research
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    • "Among the most well known effects, it has been reported that triclosan impairs algal growth and develops bacterial resistance (Orvos et al., 2002) whereas pharmaceutical mixtures containing carbamazepine, ibuprofen, and clofibric acid have also been found to be toxic for algae (Cleuvers, 2003). Estrogenicity has already been demonstrated for many contaminants such as natural and synthetic hormones and alkylphenols commonly detected in wastewaters (Dagnino et al., 2010). Although the removal of EOCs may be partially achieved by the application of conventional wastewater treatment plants (WWTPs) combined to advanced tertiary treatment processes (Rosal et al., 2010 ), their high cost limits the widespread application of these treatment technologies. "
    [Show abstract] [Hide abstract] ABSTRACT: The capacity of a hybrid constructed wetland (CW) system consisting of two vertical flow (VF) CWs working alternatively (3m(2)), one horizontal flow (HF) CW (2m(2)) and one surface flow (FWS) CW (2m(2)) in series to eliminate 13 emerging organic contaminants (EOCs) under three different hydraulic loading rates (HLRs) (0.06, 0.13 and 0.18m d(-1) considering the area of the two VF beds) was studied through a continuous injection experiment. General toxicity, dioxin-like activity, antimicrobial activity and estrogenicity were also measured under the highest hydraulic loading rate. The hybrid system was highly efficient on the removal of total injected EOCs (except for antibiotics, 43±32%) at all three HLRs (87±10%). The removal efficiency in the hybrid CW system showed to decrease as the HLR increased for most compounds. The VF wetlands removed most of the injected EOCs more efficiently than the other two CWs, which was attributable to the predominant aerobic degradation pathways of the VF beds (70±21%). General toxicity was reduced up to 90% by the VF beds. Estrogenicity and dioxin-like activity were similarly reduced by the VF and the HF wetlands, whereas antimicrobial activity was mainly removed by the FWS wetland. Bearing this in mind, this injection study has demonstrated that the use of hybrid CW systems is a suitable wastewater technology for removing EOCs and toxicity even at high HLRs.
    Full-text · Article · Nov 2013 · Science of The Total Environment
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    • "Therefore, sample pretreatment is often required for isolating and/or concentrating the target toxic substances and eliminating the interference. When organic substances are the targets of ecotoxicity test, liquid–liquid extraction (Pérez et al., 2009), resin adsorption (Reginatto et al., 2009) and solid-phase extraction (SPE) (Dagnino et al., 2010; Smital et al., 2011) are the common pretreatment methods for effective extraction of organic substances and elimination of all inorganic interferences. If heavy metals become the target substances, passive sampling can effectively concentrate the metals ions by utilizing diffusive gradient in thin-films (Roig et al., 2011). "
    [Show abstract] [Hide abstract] ABSTRACT: Luminescent bacterial test is a fast and sensitive method for acute toxicity assessment of water and wastewater. In this study, an improved toxicity testing method was developed using the freshwater luminescent bacteria Vibrio qinghaiensis sp.-Q67 that involved pretreatment of water samples with reverse osmosis (RO) to eliminate the interferences caused by nutrients in concentrated samples and to improve the reliability and sensitivity of the analysis. Because water samples contain low concentrations of several target toxic substances, rapid acute toxicity testing method that is commonly employed does not achieve enough sensitivity. The proposed RO pretreatment could effectively enrich organic and inorganic substances in water samples to enable a more effective and sensitive toxicity evaluation. The kinetic characteristics of toxicity of raw sewage and secondary effluent were evaluated based on the relative luminescence unit (RLU) curves and time-concentration-effect surfaces. It was observed that when the exposure time was prolonged to 8-h or longer, the bacteria reached the logarithmic growth stage. Hence, the stimulating effects of the coexisting ions (such as Na(+), K(+), NO3(-)) in the concentrated samples could be well eliminated. A 10-h exposure time in proposed Q67 test was found to quantitatively evaluate the toxicity of the organic and inorganic pollutants in the RO-concentrated samples.
    Full-text · Article · Aug 2013 · Ecotoxicology and Environmental Safety
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