Development of SCAR Markers for Species Identification of the Genus Nepenthes (Nepenthaceae)

ArticleinPakistan Journal of Biological Sciences 12(22):1455-61 · November 2009with46 Reads
DOI: 10.3923/pjbs.2009.1455.1461 · Source: PubMed
Nepenthes species in Thailand, namely N. mirabilis Druce, N. gracilis Korth., N. smilesii Hemsl., N. ampullaria Jack and N. kampotiana Lecomte, were collected for development of Sequence Characterized Amplified Region (SCAR) marker, a genotype identification tool. Forty Random Amplified Polymorphic DNA (RAPD) primers were screened and three successful primers produced different banding patterns including five candidate species-specific markers. The candidate markers were cloned and sequenced. The marker sequences are 602, 379, 420, 473 and 1017 bp for N. mirabilis, N. gracilis, N. smilesii, N. ampullaria and N. kampotiana, respectively. Then the sequences were used to design primers for development of a species-specific band being a SCAR marker, including Mir 1, Mir 2 and Mir 3 for N. mirabilis; Gra 1 and Gra 2 for N. gracilis; Smi 1, Smi 2 and Smi 3 for N. smilesii; Amp 1 and Amp 2 for N. ampullaria and Kam 1 and Kam 2 of N. kampotiana. The primers were evaluated with each other Nepenthes species. Finally, species-specific SCAR markers were successfully developed for N. gracilis, N. ampullaria and N. kampotiana. Application of these markers is feasible for identification of Nepenthes species in Thailand.
    • "Recently, the development of molecular technology has provided suitable tools for DNA analysis in the field of genotoxicology. In many researches, random amplified polymorphic DNA (RAPD) is generally used to effectively indicate the genetic relationships by phylogenetic tree reconstruction (Tanee et al., 2012; Kaewdoungdee & Tanee, 2013; Noikotr et al., 2013) and species identification (Anuniwat et al., 2009). Furthermore, RAPD banding profiles can be scored for genomic template stability (GTS) analysis to detect various types of DNA damage and mutations (point mutation, rearrangement and small deletion or insertion of DNA). "
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