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Konstantinidou V, Covas MI, Muñoz-Aguayo D, Khymenets O, de la Torre R, Saez G, et al. In vivo nutrigenomic effects of virgen olive oil polyphenols within the frame of the Mediterranean diet: a randomized controlled trial. FASEB

Cardiovascular Risk and Nutrition Research Group, Institut Municipal d'Investigació Mèdica (IMIM-Hospital del Mar), Centro de Investigación Biomédica Eu Red (CIBER) de Fisiopatología de la Obesidad y Nutrición, Barcelona, Spain.
The FASEB Journal (Impact Factor: 5.04). 02/2010; 24(7):2546-57. DOI: 10.1096/fj.09-148452
Source: PubMed

ABSTRACT

The aim of the study was to assess whether benefits associated with the traditional Mediterranean diet (TMD) and virgin olive oil (VOO) consumption could be mediated through changes in the expression of atherosclerosis-related genes. A randomized, parallel, controlled clinical trial in healthy volunteers (n=90) aged 20 to 50 yr was performed. Three-month intervention groups were as follows: 1) TMD with VOO (TMD+VOO), 2) TMD with washed virgin olive oil (TMD+WOO), and 3) control with participants' habitual diet. WOO was similar to VOO, but with a lower polyphenol content (55 vs. 328 mg/kg, respectively). TMD consumption decreased plasma oxidative and inflammatory status and the gene expression related with both inflammation [INF-gamma (INFgamma), Rho GTPase-activating protein15 (ARHGAP15), and interleukin-7 receptor (IL7R)] and oxidative stress [adrenergic beta(2)-receptor (ADRB2) and polymerase (DNA-directed) kappa (POLK)] in peripheral blood mononuclear cells. All effects, with the exception of the decrease in POLK expression, were particularly observed when VOO, rich in polyphenols, was present in the TMD dietary pattern. Our results indicate a significant role of olive oil polyphenols in the down-regulation of proatherogenic genes in the context of a TMD. In addition, the benefits associated with a TMD and olive oil polyphenol consumption on cardiovascular risk can be mediated through nutrigenomic effects.

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    • "Also for these reasons, research on the anti-inflammatory properties of natural compounds is particularly active and has led to substantiate with some molecular details the already documented efficacy of EVOO and of its phenolic component in this respect. For example, significant reductions in serum leukotriene B4 and thromboxane B2 concentrations at 2 and 6 h after consumption of EVOO, but not after consumption of either olive oil or corn oil (both poor in phenols) were observed (Bogani et al. 2007); a three-month intervention with a MD characterised by EVOO as the main source of fats determined a decrease in the expression of inflammation related genes (INF-γ, Rho GTPase-activating protein-15 and interleukin-7 receptor) higher than that coming from the adoption of a MD containing an olive oil poor in phenols (Konstantinidou et al. 2010); 50 µM OLE (a concentration that was supposed to be reached in plasma by individuals consuming an EVOO-rich diet) inhibited tumour necrosis factor-α (TNF-α) induced matrix metalloproteinase 9 (MMP-9) expression and secretion in THP-1 human monocytic leukaemia cells by impairing NF-κB -mediated genes transcription (Dell'Agli et al. 2010); similar oleuropein and HT concentrations inhibited PMA-stimulated COX-2 and MMP-9 expression and activities thus reducing inflammatory angiogenesis in cultured endothelial cells (Scoditti et al. 2012); in this case too a reduction in the ROS-sensitive NF-κB transactivation was observed. However, the opposite result was obtained in a different cell type (embryonic kidney HEK293 cells stably expressing APP) where oleuropein seemed to increase MMP-9 gelatinolytic activity in the culture medium (Kostomoiri et al. 2013). "
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