Chen, Z. G. et al. Screening DNA-targeted anticancer drug in vitro based on the drug-conjugated DNA by resonance light scattering technique. Biosens. Bioelectron. 25, 1947-1952

ArticleinBiosensors & Bioelectronics 25(8):1947-52 · April 2010with14 Reads
Impact Factor: 6.41 · DOI: 10.1016/j.bios.2010.01.011 · Source: PubMed
Abstract

A sensitive and reliable assay has been developed to directly screen DNA-targeted anticancer drugs in vitro via using resonance light scattering (RLS) technique. The results of experiments displayed that the increment of RLS intensity was directly proportional to the antitumor effect of anticancer drugs. Through the RLS spectra, the activities of four drugs have been demonstrated as mitoxantrone (MIT)>epirarubicin (EPI)>daunorubicin (DAU)>adriamycin (ADM). However, to further verify the activities of the above four drugs, binding constant (k) for each of them has been calculated by RLS technique as follows: k(RLS) (MIT, 8.75 x 10(5) L mol(-1))>k(RLS) (EPI, 6.58 x 10(5) L mol(-1))>k(RLS) (DAU, 4.79 x 10(5) L mol(-1))>k(RLS) (ADM, 3.82 x 10(5) L mol(-1)). Also, this RLS assay result was validated by seasoned vitro screening methods for anticancer drugs. In all, the proposed RLS is not only a simple, sensitive, objective and straightforward method, but also it is an unprecedented assay for primarily screening DNA-targeted anticancer drugs.

    • "The light scattering signals can be easily detected by synchronously scanning both the excitation and emission monochromators with a conventional spectrofluorometer (Pasternack et al. 1993; Pasternack et al. 1995; Brar and Verma 2011; Ling et al. 2009; Ling et al. 2007). To date, RLS has been applied to the study and determination of nucleic acids (Huang et al. 1997), adenine (Xu et al. 2012), amino acid (Chen et al. 2012), and screening of anticancer drugs (Chen et al. 2010; Chen et al. 2011). With the development of nanoscience and nanotechnology, gold nanoparticles (AuNPs) based on RLS methods have been attracting enormous attention in biomolecule detection, since the light scattering ability of AuNPs is about four orders of magnitude stronger than that of a fluorescent dye (). "
    [Show abstract] [Hide abstract] ABSTRACT: In this contribution, novel chitosan-stabilized gold nanoparticles (AuNPs) were prepared by mixing chitosan with citrate-reductive AuNPs under appropriate conditions. The as-prepared chitosan-stabilized AuNPs were positively charged and highly stably dispersed in aqueous solution. They exhibited weak resonance light scattering (RLS) intensity and a wine red color. In addition, the chitosan-stabilized AuNPs were successfully utilized as novel sensitive probes for the detection of heparin for the first time. It was found that the addition of heparin induced a strong increase of RLS intensity for AuNPs and the color change from red to blue. The increase in RLS intensity and the color change of chitosan-stabilized AuNPs caused by heparin allowed the sensitive detection of heparin in the range of 0.2–60 μM (~6.7 U/mL). The detection limit for heparin is 0.8 μM at a signal-to-noise ratio of 3. The present sensor for heparin detection possessed a low detection limit and wide linear range. Additionally, the proposed method was also applied to the detection of heparin in biological media with satisfactory results. Electronic supplementary material The online version of this article (doi:10.1007/s11051-013-1930-9) contains supplementary material, which is available to authorized users.
    Preview · Article · Sep 2013 · Journal of Nanoparticle Research
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  • [Show abstract] [Hide abstract] ABSTRACT: Interactions of the anti-inflammatory drug ketoprofen with calf thymus DNA (ctDNA) in aqueous solution have been studied by multi-spectroscopic method including resonance light scattering (RLS) technique, ultraviolet spectra (UV), (1)H NMR, etc. The characteristics of RLS spectra, the effective factors and optimum conditions of the reaction have been unequivocally investigated. Mechanism investigations have shown that ketoprofen can bind to ctDNA by groove binding and form large particles, which resulted in the enhancement of RLS intensity. In Critic acid-Na(2)HPO(4) buffer (pH=6.5), ketoprofen has a maximum peak 451.5 nm and the RLS intensity is remarkably enhanced by trace amount of ctDNA due to the interaction between ketoprofen and ctDNA. The enhancement of RLS signal is directly proportional to the concentration of ctDNA in the range of 1.20×10(-6)-1.0×10(-5) mol/L, and its detection limit (3σ) is 1.33×10(-9) mol/L. The method is simple, rapid, practical and relatively free from interference generated by coexisting substance, and was applied to the determination of trace amounts of nucleic acid in synthetic samples with satisfactory results.
    No preview · Article · Feb 2011 · Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy
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  • [Show abstract] [Hide abstract] ABSTRACT: We have developed a method to investigate the interaction between DNA-targeted anthracyclines and DNA in the presence of the drug paclitaxel. It is based on resonance light scattering (RLS) and on the finding that anthracyclines when bound to DNA undergo a dramatic enhancement in their RLS intensities, while paclitaxel does not display such an effect. However, the RLS intensities of the anthracyclines-DNA associates are remarkably enhanced again on addition of paclitaxel. UV-visible spectra reveal interactions between paclitaxel and anthracyclines, but no reaction between paclitaxel and DNA. Consequently, paclitaxel, though not DNA-targeted, can improve the DNA-binding capabilities of anthracyclines. Binding constants between anthracyclines and DNA, and improved efficiency of paclitaxel on the DNA-binding capabilities of anthracyclines were calculated. The DNA binding constants of doxorubicin, epirubicin, and mitoxantrone, respectively, are 4.53 × 105 L mol−1, 6.05 × 105 L mol−1, and 9.47 × 105 L mol−1. The improved values in presence of paclitaxel are 78%, 47% and 19%. We also have investigated the effects of drug concentrations and the order of adding the drugs. Displacement studies (using methylene blue as a competitive agent) provided additional information on the mechanisms of the interaction between paclitaxel and anthracyclines. Figure A novel resonance light scattering (RLS) method for the investigation on the interaction between anthracyclines and DNA in the presence of paclitaxel has been developed based on the enhanced RLS intensities.
    No preview · Article · Apr 2011 · Microchimica Acta
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