Chen, Z. G. et al. Screening DNA-targeted anticancer drug in vitro based on the drug-conjugated DNA by resonance light scattering technique. Biosens. Bioelectron. 25, 1947-1952

Department of Chemistry, Shantou University, Daxue Road 243#, Shantou 515063, China.
Biosensors & Bioelectronics (Impact Factor: 6.41). 04/2010; 25(8):1947-52. DOI: 10.1016/j.bios.2010.01.011
Source: PubMed


A sensitive and reliable assay has been developed to directly screen DNA-targeted anticancer drugs in vitro via using resonance light scattering (RLS) technique. The results of experiments displayed that the increment of RLS intensity was directly proportional to the antitumor effect of anticancer drugs. Through the RLS spectra, the activities of four drugs have been demonstrated as mitoxantrone (MIT)>epirarubicin (EPI)>daunorubicin (DAU)>adriamycin (ADM). However, to further verify the activities of the above four drugs, binding constant (k) for each of them has been calculated by RLS technique as follows: k(RLS) (MIT, 8.75 x 10(5) L mol(-1))>k(RLS) (EPI, 6.58 x 10(5) L mol(-1))>k(RLS) (DAU, 4.79 x 10(5) L mol(-1))>k(RLS) (ADM, 3.82 x 10(5) L mol(-1)). Also, this RLS assay result was validated by seasoned vitro screening methods for anticancer drugs. In all, the proposed RLS is not only a simple, sensitive, objective and straightforward method, but also it is an unprecedented assay for primarily screening DNA-targeted anticancer drugs.

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    ABSTRACT: Interactions of the anti-inflammatory drug ketoprofen with calf thymus DNA (ctDNA) in aqueous solution have been studied by multi-spectroscopic method including resonance light scattering (RLS) technique, ultraviolet spectra (UV), (1)H NMR, etc. The characteristics of RLS spectra, the effective factors and optimum conditions of the reaction have been unequivocally investigated. Mechanism investigations have shown that ketoprofen can bind to ctDNA by groove binding and form large particles, which resulted in the enhancement of RLS intensity. In Critic acid-Na(2)HPO(4) buffer (pH=6.5), ketoprofen has a maximum peak 451.5 nm and the RLS intensity is remarkably enhanced by trace amount of ctDNA due to the interaction between ketoprofen and ctDNA. The enhancement of RLS signal is directly proportional to the concentration of ctDNA in the range of 1.20×10(-6)-1.0×10(-5) mol/L, and its detection limit (3σ) is 1.33×10(-9) mol/L. The method is simple, rapid, practical and relatively free from interference generated by coexisting substance, and was applied to the determination of trace amounts of nucleic acid in synthetic samples with satisfactory results.
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    ABSTRACT: In this contribution, a novel resonance light scattering (RLS) amplification system was developed based on the enhanced RLS signals of the ammonium molybdate (AM)–cetyl trimethyl ammonium bromide (CTMAB) system upon the addition of benzidine (BD). It was found that in the presence of CTMAB, trace amounts of BD in surface water induced a remarkable change in RLS intensity of the AM–CTMAB system. Moreover, in the range of 0.005–1.0 μg mL−1, there was a good linear relationship between the enhanced RLS intensity and the concentration of BD with a detection limit of 0.08 ng mL−1. Three kinds of surface water sample were determined by this assay, and the interaction mechanism between AM and BD as well as several influencing factors were also investigated. Compared with conventional assays for the determination of BD in water, this assay has the advantages of simplicity, high sensitivity and it is more suitable for the determination of trace amounts of micromolecules.
    No preview · Article · Aug 2011 · Analytical methods
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