Collecting back-reflected photons in
Hao F. Zhang
, Jing Wang
, Qing Wei
, Tan Liu
, Shuliang Jiao
, and Carmen A.
Department of Electrical Engineering and Computer Science, University of Wisconsin-Milwaukee, Milwaukee WI
Department of Ophthalmology, University of Southern California, Los Angeles, CA 90033, USA
College of Electronic Science and Engineering, Jilin University, Changchun, 130012, China
Abstract: Since the photoacoustic effect relies only on the absorbed optical
energy, the back-reflected photons from samples in optical-resolution
photoacoustic microscopy are usually discarded. By employing a 2 × 2
single-mode fiber optical coupler in a laser-scanning optical-resolution
photoacoustic microscope for delivering the illuminating laser light and
collecting the back reflected photons, a fiber-optic confocal microscope is
integrated with the photoacoustic microscope. Thus, simultaneous
multimodal imaging can be achieved with a single light source and images
from the two modalities are intrinsically registered. Such capabilities are
demonstrated in imaging both phantoms and small animals in vivo.
©2010 Optical Society of America
OCIS codes: (110.5120) Photoacoustic imaging; (170.1790) Confocal microscopy; (110.0180)
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#120567 - $15.00 USD Received 30 Nov 2009; revised 31 Dec 2009; accepted 4 Jan 2010; published 11 Jan 2010
(C) 2010 OSA 18 January 2010 / Vol. 18, No. 2 / OPTICS EXPRESS 1278