Light Image Patterned Molecular Delivery into Live Cells Using Gold Particle Coated Substrate

Department of Electrical Engineering, University of California, Los Angeles (UCLA), 420 Westwood Plaza, 48-121 Engineering IV, Los Angeles, CA 90095-1597, USA.
Optics Express (Impact Factor: 3.49). 01/2010; 18(2):938-46. DOI: 10.1109/LEOSST.2008.4590556
Source: PubMed


An image-patterned molecular delivery system for mammalian cells is demonstrated by pulsed laser irradiation of gold particles immobilized on a substrate below a cell monolayer. Patterned cavitation bubble nucleation was captured using a time-resolved imaging system and molecular delivery verified by observing the uptake of a membrane-impermeable fluorescent dye, calcein. Delivery efficiency as high as 90% was observed and multiplexed, patterned dye delivery was demonstrated. (C) 2009 Optical Society of America

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