A simple and sensitive HPLC method for the simultaneous determination of eight bioactive components and fingerprint analysis of Schisandra sphenanthera

Department of Pharmacy, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Analytica chimica acta (Impact Factor: 4.51). 03/2010; 662(1):97-104. DOI: 10.1016/j.aca.2009.12.039
Source: PubMed


A simple and sensitive high performance liquid chromatography method with photodiode array detection (HPLC-DAD) was developed for simultaneous determination of eight bioactive constituents (schisandrin, schisandrol B, schisantherin A, schisanhenol, anwulignan, deoxyshisandrin, schisandrin B and schisandrin C) in the ripe fruit of Schisandra sphenanthera and its traditional Chinese herbal preparations Wuzhi-capsule by optimizing the extraction, separation and analytical conditions of HPLC-DAD. The chemical fingerprint of S. sphenanthera was established using raw materials of 15 different origins in China. The chromatographic separations were obtained by an Agilent Eclipse XDB-C18 reserved-phase column (250 mm x 4.6 mm i.d., 5 microm) using gradient elution with water-formic acid (100:0.1, v/v) and acetonitrile, at a flow rate of 1.0 mL min(-1), an operating temperature of 35 degrees C, and a wavelength of 230 nm. The constituents were confirmed by (+) electrospray ionization LC-MS. The new method was validated and was successfully applied to simultaneous determination of components in 13 batches of Wuzhi-capsule. The results indicate that this multi-component determination method in combination with chromatographic fingerprint analysis is suitable for quantitative analysis and quality control of S. sphenanthera.

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Available from: Wan-Sheng Chen, Nov 06, 2014
    • "In the process, techniques such as HPLC, (gas chromatography) GC, gas chromatography-mass spectrometry (GC-MS), and liquid chromatography-mass spectrometry (LC-MS) are often used. However, HPLC is simple, reliable, and inexpensive, and has been widely used for quantitative analysis of herbal medicine.[1112131415] "
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    ABSTRACT: Background:Tilia amurensis consists of various compounds, such as flavonoids and terpenoids.Objective:A simple and reliable high performance liquid chromatography (HPLC) coupled with the diode array detector (DAD) method has been established for simultaneous determination of epicatechin, nudiposide, lyoniside, and scopoletin isolated from Tilia amurensis.Materials and Methods:Optimum separations were obtained with a SHISEIDO C18 column by gradient eluton, with 0.1% Trifluoroacetic acid (TFA) water-methanol as the mobile phase. The gradient elution system was completed within 40 minutes. The flow rate and detection wavelength were 1 mL/minute, 205 nm, 250 nm, and 280 nm, respectively.Results:Validation of the analytical method was evaluated by linearity, precision, and the accuracy test. The calibration curve was linear over the established range with R2 > 0.997. The limit of detection (LOD) and limit of quantification (LOQ) ranged from 0.01-15.20 μg/mL and 0.03-46.06 μg/mL. The method exhibited an intraday and interday precision range of 96.25-105.66% and 93.52-109.92%, respectively (RSD <2.80%). The recoveries and relative standard deviation (RSD) of the four compounds in Tilia amurensis were in the range of 90.42-104.84% and 0.2-2.58%.Conclusion:This developed method was accurate and reliable for the quality evaluation of the four compounds isolated from Tilia amurensis.
    No preview · Article · Jul 2014 · Pharmacognosy Magazine
    • "All validation was showed for determination of analysis sample in this study. Linearity, limit of detection (LOD), limit of quantification (LOQ), precision, and recovery were evaluated for the method.[12131415] "
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    • "Previously, our laboratory has screened the CYP3A substrates from a large number of Traditional Chinese Medicines that are classified as superior drugs (Wu et al., 2012). Fructus Schisandrae has been used for thousands of years, owing to its diverse pharmacological effects (Wei et al., 2010). Many structurally similar dibenzocyclooctadiene lignans from Fructus Schisandrae were found to be metabolized by CYP3A4. "
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