A simple and sensitive HPLC method for the simultaneous determination of eight bioactive components and fingerprint analysis of Schisandra sphenanthera

Article (PDF Available)inAnalytica chimica acta 662(1):97-104 · March 2010with145 Reads
DOI: 10.1016/j.aca.2009.12.039 · Source: PubMed
Abstract
A simple and sensitive high performance liquid chromatography method with photodiode array detection (HPLC-DAD) was developed for simultaneous determination of eight bioactive constituents (schisandrin, schisandrol B, schisantherin A, schisanhenol, anwulignan, deoxyshisandrin, schisandrin B and schisandrin C) in the ripe fruit of Schisandra sphenanthera and its traditional Chinese herbal preparations Wuzhi-capsule by optimizing the extraction, separation and analytical conditions of HPLC-DAD. The chemical fingerprint of S. sphenanthera was established using raw materials of 15 different origins in China. The chromatographic separations were obtained by an Agilent Eclipse XDB-C18 reserved-phase column (250 mm x 4.6 mm i.d., 5 microm) using gradient elution with water-formic acid (100:0.1, v/v) and acetonitrile, at a flow rate of 1.0 mL min(-1), an operating temperature of 35 degrees C, and a wavelength of 230 nm. The constituents were confirmed by (+) electrospray ionization LC-MS. The new method was validated and was successfully applied to simultaneous determination of components in 13 batches of Wuzhi-capsule. The results indicate that this multi-component determination method in combination with chromatographic fingerprint analysis is suitable for quantitative analysis and quality control of S. sphenanthera.
    • "e concentrations of all standard solutions under the optimized conditions within a day. Inter-day precision was validated with the standard solutions for three consecutive days. Repeatability was conducted by analyzing the extractive solution six times in the same day. All investigated components were expressed as relative standard deviation (RSD). [10] The newly established analytical method was subsequently applied for the simultaneous determination of six isolated compounds in the methanol extract of CA. The isolated compounds were quantified by linear regression of the standards. Each compound was analyzed in triplicate to determine the mean content."
    [Show abstract] [Hide abstract] ABSTRACT: Hyangsapyeongwisan (HSPWS), known as traditional herbal medicine, has been used in the treatment of gastric disease. Standardization of HSPWS is a necessary step for the establishment of a consistent biological activity for the production and manufacturing of HSPWS herbal preparations. A simple, sensitive and accurate method using ultra-performance liquid chromatography (UPLC) fingerprinting with a diode array detector (DAD) was developed and validated for systematic quality evaluation of HSPWS. Separation conditions were optimized using a Halo C18 2.7 µm, 4.6 × 100 mm column with a mobile phase of 0.1% phosphoric acid and acetonitrile, and detection wavelengths of 215, 250 and 350 nm. Validation of the analytical method was evaluated by tests of linearity, precision, accuracy and robustness. All calibration curves of components showed good linearity (R(2) > 0.9996). The limit of detection (LOD) and limit of quantification (LOQ) were within the ranges of 0.004-0.134 and 0.012-0.406 µg/mL, respectively. The relative standard deviation (RSD) values of intra- and inter-day testing were within the range of 0.01-3.84%. The result of the recovery test was 96.82-104.62% with an RSD value of 0.14-3.84%. Robustness values of all parameters as well as the stability test of analytical solutions were within the standard limit. It showed that the developed method was simple, specific, sensitive, accurate, precise, reproducible and robust for the quantification of active components of HSPWS. Chromatographic fingerprinting with quantitative analysis of marker compounds in HSPWS prepared by different methods and commercial formulation was also evaluated successfully.
    Article · Dec 2015
    • "e concentrations of all standard solutions under the optimized conditions within a day. Inter-day precision was validated with the standard solutions for three consecutive days. Repeatability was conducted by analyzing the extractive solution six times in the same day. All investigated components were expressed as relative standard deviation (RSD). [10] The newly established analytical method was subsequently applied for the simultaneous determination of six isolated compounds in the methanol extract of CA. The isolated compounds were quantified by linear regression of the standards. Each compound was analyzed in triplicate to determine the mean content."
    [Show abstract] [Hide abstract] ABSTRACT: Cheilanthes albomarginata Clarke, a fern from the Pteridaceae family, is traditionally used to treat peptic ulcer and stomach disorder. Six flavonoid compounds were isolated and identified from the ethyl acetate soluble fraction of this plant. Kumatakenin 5-O-β-glucoside,kaempferol-7-methyl ether, rhamnocitrin 3-O-β-glucoside, and kaempferol 3-O-β-glucoside were isolated for the first time from this plant. UPLC method was developed and used for the simultaneous determination and quantification of the isolated compounds in the methanol extract of the plant. The method was also validated using the parameters: linearity, precision, and accuracy.
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    • "Comprehensive fingerprint profiles were performed by the " Similarity Evaluation System for Chromatographic Fingerprint of TCM " software (SESC-TCM, Version 2004 A, Chinese Pharmacopoeia Commission) [25,26]. The software can export the reference chromatogram based on correlation coefficients (R1); the R1 was calculated with median or average data and was expressed as follows [27]: "
    [Show abstract] [Hide abstract] ABSTRACT: Notopterygii Rhizoma et Radix (Qianghuo), including Notopterygium incisum Ting ex H. T. Chang (NI) and Notopterygium franchetii H. de Boiss (NF), is an important traditional Chinese medicine. Of these two plants, NI, is more commonly used and has a much higher price in the marketplace. To compare these two plants, a combination of gas chromatography-mass spectrometry (GC-MS) and high performance liquid chromatography (HPLC) was carried out, thus obtaining an overall characterization for both volatile and none-volatile compounds. Combined with hierarchical cluster analysis (HCA) and principal component analysis, GC-MS was successfully applied to distinguish NF and NI. The chemical constitutes of volatile oil in NI and NF were firstly compared in detail, and 1R-alpha-pinene, beta-pinene and 4-isopropyl-1-methyl-1,4-cyclohexadiene had great contribution to the discrimination. Fingerprints of 14 batches of Qinghuo samples were also established based on HPLC, and an obvious difference was found between the two species. The chromatographic fingerprints were further analyzed by similarity analysis and HCA. The present study is the first reported evaluation of two origins of Notopterygii Rhizoma et Radix by GC-MS and HPLC, which will facilitate quality control and its clinical application.
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