Biodegradation of the major color containing compounds in distillery wastewater by an aerobic bacterial culture and characterization of their metabolites

Environmental Microbiology Section, Indian Institute of Toxicology Research , Lucknow, Uttar Pradesh, India.
Biodegradation (Impact Factor: 2.34). 02/2010; 21(5):703-11. DOI: 10.1007/s10532-010-9336-1
Source: PubMed


This study deals the biodegradation of the major color containing compounds extracted from distillery wastewater (DWW) by an aerobic bacterial consortium comprising Bacillus licheniformis (DQ79010), Bacillus sp. (DQ779011) and Alcaligenes sp. (DQ779012) and characterization of metabolic products. The degradation of color containing compounds by bacteria was studied by using the different carbon and nitrogen sources at different environmental conditions. Results revealed that the bacterial consortium was efficient for 70% color removal in presence of glucose (1.0%) and peptone (0.1%) at pH 7.0 and temperature 37 degrees C. The HPLC analysis of control and bacterial degraded samples has shown the reduction in peak area as well as shifting of peaks compared to control indicating the bacterial degradation as well as transformation of color containing compounds from DWW. The comparative LC-MS-MS and other spectrophotometric analysis has shown the presence of dihydroxyconiferyl alcohol, 2, 2'-bifuran-5-carboxylic acid, 2-nitroacetophenone, p-chloroanisol, 2, 3-dimethyl-pyrazine, 2-methylhexane, methylbenzene, 2, 3-dihydro-5-methylfuran, 3-pyrroline, and acetic acid in control samples that were biodegraded and biotransformed into 2-nitroacetophenone, p-chloroanisol, 2, 2'-bifuran, indole, 2-methylhexane, and 2, 3-dihydro-5-methylfuran by bacterial consortium. In this study, it was observed that most of the compounds detected in control samples were diminished from the bacterial degraded samples and compounds 2, 2'-bifuran and indole with molecular weight 134 and 117 were produced as new metabolites during the bacterial degradation of color containing compounds from DWW.

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Available from: Ram Naresh Bharagava, Dec 17, 2013
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    • "Various methods have been tried to remove melanoidin color and recalcitrant COD. Biological processes employing bacteria [9], fungi [10], and enzyme [11] have been tried before to treat melanoidin-rich wastewater. Though biological processes are generally less costly than chemical or physical treatment methods, none of the reported methods can effectively remove the contaminants in melanoidin-rich wastewaters. "
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    ABSTRACT: Melanoidin-rich industrial effluents, e.g. from coffee extraction plants and molasses distilleries, can cause potential environmental problems due to the high content of remnant dissolved organic carbon and dark color. It mainly consists of melanoidins and other organic colorants, which are recalcitrant to biological treatment. The current study was aimed to develop a polishing step after anaerobic digestion for the colorant elimination from melanoidin-rich wastewater (molasses distillery wastewater, MDW) using natural manganese oxides. Anaerobically digested MDW was used to test the removal of organic contents and color at different pH values. It was observed that the kinetics of colorant elimination was best described by the second order equation, with a significant dependence on pH. Furthermore, the liquid chromatography with organic carbon detection was applied to analyze the changes in molecular composition during the reaction. There was a preferential removal of low weight melanoidin molecules over higher weight molecules.
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    • "). Most of the metabolic products have been also reported from distillery effluent by fungal treatment (Gonzalez et al., 2000) and also as degradation products of synthetic and natural melanoidin after bacterial degradation as published in earlier studies (Bharagava and Chandra, 2010; Chandra et al., 2009). "
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    • "Wastewater treatment by conventional biological treatment leads to the large reduction in the organic load but colorants are scarcely degraded. The removal and degradation of the color contributing compounds like melanoidins, caramel, and phenolics and their metabolic products is very essential for the safe disposal of the distillery wastewater to the environmental [13]. Many researchers have tried to use biological processes for removing melanoidins from effluent. "
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