Sex-preselected buffalo (Bubalus bubalis) calves derived from artificial insemination with sexed sperm

Animal Reproduction Institute, Guangxi Key Laboratory of Subtropical Bio-resource Conservation and Utilization, Guangxi University, Nanning, PR China.
Animal reproduction science (Impact Factor: 1.51). 06/2010; 119(3-4):169-71. DOI: 10.1016/j.anireprosci.2010.01.001
Source: PubMed


Flow cytometry sorting of X- and Y-chromosome bearing sperm has been emerging as a promising technology to alter the sex ratio in progenies of mammals in the recent years. The objective of this study was to evaluate the efficiency of AI by using the sexed sperm to produce sex-preselected calves in buffalo species. A total of 43 buffalo cows were inseminated with X-sorted sperm, 30 of which were confirmed pregnant 3 mo following AI. In terms of conception rate, significant difference was observed between AI with sexed sperm derived from different bulls (P<0.05), but not between sexed and non-sexed sperm (P>0.05), nor between heifers and parous buffalo cows (P>0.05). A total of 29 sex-preselected calves, 24 females and 5 males, developed to term and were viable on delivery. Results of this study indicate the feasibility of the application of the sexing technology to accelerate the genetic improvement in swamp buffalo.

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Available from: Sheng-Sheng Lu, Dec 16, 2013
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    • "Recently, flow-cytometry sorting of X-and Yspermatozoa followed by insemination for production of sex-preselected offspring as a breeding strategies has been successful in domestic animals, such as horse (Buchanan et al., 2000), cattle (de Graaf et al., 2009), pig (Grossfeld et al., 2005) and sheep (Hollinshead et al., 2002). The same technique has been used in buffalo and buffalo calves were born after in vitro fertilization and artificial insemination with sorted buffalo spermatozoa (Presicce et al., 2005; Lu et al., 2006, 2007, 2010). Application of this technology will accelerate the expansion of high genetic merit in swamp buffalo and boost the meat and milk production industry. "
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    ABSTRACT: Flow-cytometry sorting technology has been successfully used to separate the X- and Y-chromosome bearing spermatozoa for production of sex-preselected buffalo. However, an independent technique should be employed to validate the sorting accuracy. In the present study, X-chromosomes of bovine were micro-dissected from the metaphase spreads by using glass needles. Then X-chromosomes were then amplified by PCR and labelled with Cy3-dUTP for use as a probe in hybridization of the unsorted and sorted buffalo spermatozoa -chromosome. The results revealed that 47.7% (594/1246) of the unsorted buffalo spermatozoa were positive for X- chromosome probe, which was conformed to the sex ratio in buffalo (X:Y spermatozoa=1:1); 9.6% (275/2869) of the Y-sorted buffalo spermatozoa and 86.1% (1529/1776) of the X-sorted buffalo spermatozoa showed strong X-chromosome FISH signals. Flow cytometer re-analysis revealed that the proportions of X- and Y-bearing spermatozoa in the sorted X and Y semen was 89.6% and 86.7%, respectively. There were no significant differences between results assayed by flow-cytometry re-analysis and by FISH in this study. In conclusion, FISH probe derived from bovine X- chromosomes could be used to verify the purity of X and Y sorted spermatozoa in buffalo.
    Full-text · Article · May 2011 · Animal reproduction science
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    • "In this study, a straw containing total 2 million live sorted spermatozoa has been found sufficient in obtaining similar rates of pregnancy when compared with straw content of 20 million nonsexed spermatozoa . Also, in contrast to the report of Lu et al. [51], the season of the year of increasing daylight did not undermine the final pregnancy outcome in our study. Additional important information derived from this study comes from the feasibility of achieving pregnancy rates with sexed semen similar to what is usually obtained with conventional semen, without the use of special precautions or inserting devices and particular skills [50]. "
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    ABSTRACT: The use of sexed semen in farm animal production and genetic improvement has been shown to be feasible with variable degree of efficiency in a number of species, and proved to be economically viable in cattle. In the last two decades, various newly developed reproductive technologies applicable in buffaloes have mushroomed. Recently, following the birth of the first buffalo calves using AI with sexed semen, commercial interest to exploit sexing of semen in this species too is aroused. In order to verify the successful adoption of this technology in the buffalo, the present study on the use of sexed semen for AI was carried out and compared with conventional artificial insemination using nonsexed semen. A total of 379 buffalo heifers were used for synchronization of ovulation using the Presynch protocol in the South of Italy. Selected animals at the time of AI were randomly allocated to three different experiment groups: (1) 102 animals subjected to AI in the body of the uterus with sexed semen (SS body); (2) 104 animals subjected to AI in the horn of the uterus with sexed semen (SS horn); and (3) 106 animals subjected to AI in the body of the uterus with conventional nonsexed semen (NSS body). Semen of three buffalo bulls was sexed by a collaborating company and commercially distributed in 0.25 mL straws with a total of 2 million sexed spermatozoa. Pregnancy rates were first assessed at Day 28 following AI, and rechecked at Day 45 by ultrasound. Pregnancy rates were nonsignificantly different between animals inseminated with sexed or nonsexed semen: 80/206 (38.8%) and 40/106 (37.7%), respectively (P = 0.85). However, site of insemination of sexed semen affected pregnancy rate significantly as higher pregnancy rates were obtained when sexed semen was deposited into the body rather than the horn of the uterus: 46/101 (45.5%) and 34/105 (32.3%), respectively (P = 0.05). In conclusion, the use of sexed semen in buffalo heifers gave satisfactory and similar pregnancy rates when compared with conventional nonsexed semen. Deposition of sexed semen into the body of the uterus, however, increased pregnancy rates significantly.
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