Article

PCR-based detection of Leishmania major kDNA within naturally infected Phlebotomus papatasi in southern Iran

Department of Medical Entomology, Research Centre for Health Sciences, School of Health and Nutrition, Shiraz University of Medical Sciences, P.O. Box 71645-111, Shiraz, Iran.
Transactions of the Royal Society of Tropical Medicine and Hygiene (Impact Factor: 1.84). 06/2010; 104(6):440-2. DOI: 10.1016/j.trstmh.2010.01.008
Source: PubMed

ABSTRACT

The annual incidence of cutaneous leishmaniasis (CL) in Iran rose by 43% over a five year period, from 2002 to 2006; most of these cases were caused by Leishmania major. Two complementary standard and nested polymerase chain reactions (PCRs) were used to detect parasites within their natural vector, Phlebotomus papatasi. Twelve different sand fly species were morphologically identified. The most abundant species (31.3%) was P. papatasi. Leptomonads were found in nine (2.4%) phlebotomines. Twenty (5.3%) sand fly species were found positive for Leishmania-genus DNA using standard PCR. The infection rate of this species was 5% and 7% by microscopic and molecular methods, respectively.

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    • "About 0.7–1.3 million cases of CL occur in 88 countries annually (Desjeux 2001). CL appears to be a major health concern, which, despite its notifiable status, necessitates urgent action to contain its increasingly reported cases (Abai et al. 2007, Azizi et al. 2010, 2012a-d, Fakoorziba et al. 2011). More than a quarter of all CL cases (n= 26,824) registered in 2010 within the Eastern Mediterranean region were Iranians (Postigo 2010). "

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    • "amplify the variable area of the DNA of Leishmania spp. in the rodent liver, spleen, ear and footpad as previously described [17] . The primer sequences were as follow; CSB1XR (reverse for 1st round): (5'-ATT TTT CGC GAT TTT CGC AGA ACG-3), CSB2XF (forward for 1st round): (5'- CGAGTAGCAGAAACTCCCGTTCA-3'). "
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    DESCRIPTION: Objective: To examine the fauna of rodents as zoonotic cutaneous leishmaniasis reservoir hosts in Zarqan County, Fars Province, south of Iran, during 2012. Methods: During 2012, wild rodents from different parts of this region were caught by Sherman traps and checked by the examination of liver and spleen smears, forLeishmania infection, to see which species were acting as reservoir hosts; the slides were then processed to extract DNA for molecular test using PCR assay. Results: From 108 rodent species caught, 63% were male and 37% identified as female. Meriones libycus was the most abundant species caught (80.5%) and 5.7% of them were found to be smearpositive forLeishmania amastigotes. The other species were Rattus rattus (14.8%) and Mus musculus (4.7%), but none of them were found positive. Leishmania infection was observed in male and female samples microscopically. Moreover, molecular results revealedLeishmania major in three male and two female specimens. Conclusions: Based on our knowledge, Meriones libycus is incriminated as the main reservoir hosts of Leishmania major in the rural area of Zarqan.
    Full-text · Research · Aug 2015
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    • "amplify the variable area of the DNA of Leishmania spp. in the rodent liver, spleen, ear and footpad as previously described [17] . The primer sequences were as follow; CSB1XR (reverse for 1st round): (5'-ATT TTT CGC GAT TTT CGC AGA ACG-3), CSB2XF (forward for 1st round): (5'- CGAGTAGCAGAAACTCCCGTTCA-3'). "
    [Show description] [Hide description]
    DESCRIPTION: Objective: To examine the fauna of rodents as zoonotic cutaneous leishmaniasis reservoir hosts in Zarqan County, Fars Province, south of Iran, during 2012. Methods: During 2012, wild rodents from different parts of this region were caught by Sherman traps and checked by the examination of liver and spleen smears, forLeishmania infection, to see which species were acting as reservoir hosts; the slides were then processed to extract DNA for molecular test using PCR assay. Results: From 108 rodent species caught, 63% were male and 37% identified as female. Meriones libycus was the most abundant species caught (80.5%) and 5.7% of them were found to be smearpositive forLeishmania amastigotes. The other species were Rattus rattus (14.8%) and Mus musculus (4.7%), but none of them were found positive. Leishmania infection was observed in male and female samples microscopically. Moreover, molecular results revealedLeishmania major in three male and two female specimens. Conclusions: Based on our knowledge, Meriones libycus is incriminated as the main reservoir hosts of Leishmania major in the rural area of Zarqan.
    Full-text · Research · Aug 2015
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