Dietary Milk Fat Globule Membrane Reduces the Incidence of Aberrant Crypt Foci in Fischer-344 Rats

Department of Nutrition, Dietetics and Food Sciences, Utah State University, 750 N 1200 E, Logan, Utah 84322-8700, USA.
Journal of Agricultural and Food Chemistry (Impact Factor: 2.91). 02/2010; 58(4):2157-63. DOI: 10.1021/jf903617q
Source: PubMed


Milk fat globule membrane (MFGM) is a biopolymer composed primarily of membrane proteins and lipids that surround the fat globules in milk. Although it is considered to have potential as a bioactive ingredient, few feeding studies have been conducted to measure its potential benefits. The aim of this investigation was to determine if dietary MFGM confers protection against colon carcinogenesis compared to diets containing corn oil (CO) or anhydrous milk fat (AMF). Male, weanling Fischer-344 rats were randomly assigned to one of three dietary treatments that differed only in the fat source: (1) AIN-76A diet, corn oil; (2) AIN-76A diet, AMF; and (3) AIN-76A diet, 50% MFGM, 50% AMF. Each diet contained 50 g/kg diet of fat. With the exception of the fat source, diets were formulated to be identical in macro and micro nutrient content. Animals were injected with 1,2-dimethylhydrazine once per week at weeks 3 and 4, and fed experimental diets for a total of 13 weeks. Over the course of the study dietary treatment did not affect food consumption, weight gain or body composition. After 13 weeks animals were sacrificed, colons were removed and aberrant crypt foci (ACF) were counted by microscopy. Rats fed the MFGM diet (n = 16) had significantly fewer ACF (20.9 +/- 5.7) compared to rats fed corn oil (n = 17) or AMF (n = 16) diets (31.3 +/- 9.5 and 29.8 +/- 11.4 respectively; P < 0.05). Gene expression analysis of colonic mucosa did not reveal differential expression of candidate colon cancer genes, and the sphingolipid profile of the colonic mucosa was not affected by diet. While there were notable and significant differences in plasma and red blood cell lipids, there was no relationship to the cancer protection. These results support previous findings that dietary sphingolipids are protective against colon carcinogenesis yet extend this finding to MFGM, a milk fat fraction available as a food ingredient.

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    • "Because components derived from the MFGM have shown properties beneficial to health, interest has increased in recent years in the isolation of the MFGM as a functional ingredient. For example, isolates from cream and buttermilk have antiproliferative activity against colon cancer cells both in vitro and in vivo (Snow et al., 2010; Zanabria et al., 2013), and immunomodulatory properties (Zanabria et al., 2014b). Processing of milk, cream, or buttermilk may affect the structure and composition of the MFGM (Kim and Jimenez Flores, 1995; Michalski et al., 2002; Morin et al., 2007). "
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    ABSTRACT: The present work evaluated the effect of processing on the antiproliferative activities of milk fat globule membrane (MFGM) extracts. The antiproliferative activity on human adenocarcinoma HT-29 cells of untreated MFGM extracts were compared with those extracted from pasteurized cream, thermally treated cream, or cream subjected to pulsed electrical field (PEF) processing. The PEF with a 37 kV/cm field strength applied for 1,705 μs at 50 and 65°C was applied to untreated cream collected from a local dairy. Heating at 50 or 65°C for 3 min (the passage time in the PEF chamber) was also tested to evaluate the heating effect during PEF treatments. The MFGM extracted from pasteurized cream did not show an antiproliferative activity. On the other hand, isolates from PEF-treated cream showed activity similar to that of untreated samples. It was also shown that PEF induced interactions between β-lactoglobulin and MFGM proteins at 65°C, whereas the phospholipid composition remained unaltered. This work demonstrates the potential of PEF not only a means to produce a microbiologically safe product, but also as a process preserving the biofunctionality of the MFGM. Copyright © 2015 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.
    No preview · Article · Feb 2015 · Journal of Dairy Science
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    • "The MFGM fractions have beneficial properties such as inhibition of growth of cancer cells (Snow et al., 2010), inhibition of pathogen adhesion (Guri et al., 2012), or antimicrobial properties (Spitsberg, 2005; Sanchez- Juanes et al., 2009). For example, fat globules from both bovine and goat milk inhibit the adhesion of Salmonella enteritidis to HT-29 human adenocarcinoma cells in a cultured cell experiment (Guri et al., 2012). "
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    ABSTRACT: Milk is composed of a wide variety of molecules with nutritional and multiple biological functionalities. The two main examples of colloidal structures in milk with multiple functions are fat globules and protein assemblies, referred to as casein micelles. Both assemblies are examples of how nature has resolved the task of delivering nutrients. They are often studied as a model of how to create structures in foods with enhanced functionality during consumption and digestion. Proteins and lipid aggregates in milk encode bioactive structures that will become important once digested, and will serve protective roles in the gut, for example, by regulating the immune system, by serving as cell differentiation factors, or by showing symbiotic functions with beneficial microorganisms. The milk components can form supramolecular structures that can be employed for the delivery of additional health benefits. The current knowledge of such structures present in milk, their changes during processing and digestion, and their relationship to biological functions in the gastrointestinal tract are outlined in this chapter. Recent findings in this area have created a paradigm shift in how we process and design new dairy foods aimed at providing additional health benefits to consumers.
    Full-text · Chapter · Jun 2014
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    • "Upon consideration of these protein solubility and AMF processing factors and in view of an apparent absence of published AMF protein data, our objective was to develop a method for reliably estimating the protein concentration in AMF and to apply the method to commercial AMF. The Kjeldahl nitrogen methodology (DePeters and Ferguson 1992) was not regarded as a suitable option due to sensitivity considerations and to the established presence of phospholipids in AMF (Niranjan and Krishnakantha 2001; Rombaut et al. 2006; Fedotova and Lencki 2008; Gallier et al. 2010; Snow et al. 2010), i.e., their contribution of nonprotein nitrogen (NPN) to a Kjeldahl N total may be expected to impart a significant positive bias. Accordingly, on the basis of (a) its capacity for excluding the NPN bias, (b) its established use for accurately determining protein concentration (Schegg et al. 1997), (c) its application to the determination of protein in oils (Hidalgo et al. 2001; Martin-Hernandez et al. 2008), (d) its ability via the use of derivatization reagents (e.g., FMOC) for quantifying low analyte concentrations (Jambor and Molnar- Perl 2009), and (e) its compatibility with widely available liquid chromatography instrumentation, amino acid analysis was selected as the methodology of choice. "
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    ABSTRACT: A method for the estimation of protein in anhydrous milk fat is described. The protein concentration is estimated by quantifying the arginine and aspartic acid (ASX) released upon acid hydrolysis of the anhydrous milk fat. Arginine and aspartic acid are derivatized with the fluorescent tag 9-fluorenylmethoxycarbonyl (FMOC), the derivatives are quantified by reversed phase HPLC, and the protein concentration in the anhydrous milk fat is estimated as 8.77 × (Arg + ASX). Method suitability was defined by experimental assessments of FMOC-arginine and FMOC-aspartic acid linearity (R 2 averages > 0.999), protein estimate precision [day-to-day RSD values (n = 8 days) ranged from 7.3 to 14 % for protein concentrations of 9.42 to 40.0 mg/kg of anhydrous milk fat], protein estimate accuracy [spike recovery average = 95.6 % (9.3 %), n = 8, spiking level = 20 mg/kg and agreement of the experimental protein estimate for butter, 0.611 (0.044) g/100 g, n = 3 lots, with a published value 0.6/100 g], and analyte selectivity (baseline resolution of FMOC-arginine and FMOC-aspartic acid from the FMOC derivatives of other common amino acids). The method provides for a reliable estimation of the protein content of anhydrous milk fat, when present at concentrations >2 mg/kg.
    Full-text · Article · Apr 2013 · Food Analytical Methods
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