Proteins That Underlie Neoplastic Progression of Ulcerative Colitis.

Department of Medicine, University of Washington, Seattle, WA 98195, USA.
PROTEOMICS - CLINICAL APPLICATIONS (Impact Factor: 2.96). 11/2009; 3(11):1326. DOI: 10.1002/prca.200900061
Source: PubMed


Patients with ulcerative colitis (UC) have an increased risk for developing colorectal cancer. Because UC tumorigenesis is associated with genomic field defects that can extend throughout the entire colon, including the non-dysplastic mucosa; we hypothesized that the same field defect will include abnormally expressed proteins. Here we applied proteomics to study the protein expression of UC neoplastic progression. The protein profiles of colonic epithelium were compared from 1) UC patients without dysplasia (non-progressors); 2) none-dysplastic colonic tissue from UC patient with high-grade dysplasia or cancer (progressors); 3) high-grade dysplastic tissue from UC progressors and 4) normal colon. We identified protein differential expression associated with UC neoplastic progression. Proteins relating to mitochondria, oxidative activity, calcium-binding proteins were some of interesting classes of these proteins. Network analysis discovered that Sp1 and c-myc proteins may play roles in UC early and late stages of neoplastic progression, respectively. Two over-expressed proteins in the non-dysplastic tissue of UC progressors, CPS1 and S100P, were further confirmed by IHC analysis. Our study provides insight into the molecular events associated with UC neoplastic progression, which could be exploited for the development of protein biomarkers in fields of non-dysplastic mucosa that identify a patient's risk for UC dysplasia.

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Available from: Tatiana Nikolskaya, Jun 06, 2014
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    • "Several S100 protein family members are differentially expressed in inflammatory bowel diseases, ie, ulcerative colitis and Crohn’s disease, and these have been implicated in inflammation-induced colorectal carcinogenesis (115). Brentnall et al. detected increased expression profiles of S100P, S100A6, S100A11, and S100H during the course of malignant transformation (116). On the other hand, S100A8, S100A9, S100A10, and S100A4 displayed heterogeneous expression patterns in ulcerative colitis patients showing neoplasia (116). "
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