Distribution of adipose-derived stem cells in adipose tissues from human cadavers
Department of Plastic and Reconstructive Surgery, Keio University School of Medicine, 35 Shinanomachi Shinjuku-ku Tokyo, 160-8582, Japan. Journal of Plastic Reconstructive & Aesthetic Surgery
(Impact Factor: 1.42).
10/2010; 63(10):1717-22. DOI: 10.1016/j.bjps.2009.10.020
Adipose-derived stem cells (ASCs) possess multipotency in vivo and in vitro, and thus are thought to be very promising precursors for use in regenerative medicine. ASCs can be concentrated from adipose tissue by enzymatic digestion and transplanted to increase angiogenesis or for cosmesis. ASC transplants are now being performed in a clinical setting. Although data on ASCs are extensive, the distribution of ASCs in human fat tissue has not been fully clarified. Thus, it is important to identify the distribution of ASCs to obtain cell populations rich in ASCs for clinical use.
ASCs express CD34, a cell surface marker. As CD34 is also expressed by endothelial cells, we immunohistochemically stained 2-μm-thick serial paraffin sections of fat tissue obtained from various parts of formalin-fixed cadavers with anti-CD31 and anti-CD34 antibodies to distinguish ASCs from endothelial cells.
CD34(+)/CD31(-) cells were mainly found in connective tissue tracts and perivascularly. Among fat tissues obtained from various sites, fat tissues in the thoracic back and lower abdomen were richest in CD34(+)/CD31(-) cells.
The concentrations of CD34(+)/CD31(-) cells in adipose tissues differ between sites. The sites most highly enriched for ASCs were identified, and it is now possible to select the best sites for collection of ASCs for transplantation.
Available from: Mehmet Tokac
- "Also adipose derived stem cells (ASCs) are available in adipose tissue (Kishi et al., 2010; Ulicna et al., 2010). ASCs in vivo are transformed into endothelial cells and they organize and develope angiogenesis. "
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ABSTRACT: Adipose tissue is a dynamic ‘hard-working’ tissue that awaits extensive studies. It does not merely function as a fat-storage
region; adipose tissue also plays a major role in the formation of body shapes, which determine attractiveness in humans.
Moreover, adipose tissue secretes molecules that direct brain processes.
The importance of adipose tissue is not limited to its physiologic roles; it also has a wide potential for use in the treatment
of several disorders, and is also the most abundant, reproducible, and accessible tissue for cell therapists. With the support
of basic and clinical studies, the significance of adipose tissue might be widely acknowledged in the near future.
Available from: Jesus Benito-Ruiz
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ABSTRACT: Though adipose tissue grafts have been used for decades in last century, the technique has been considered as slightly usefull provided that the graft was tending to reabsorption. It was Coleman who introduced a system of atraumatic harvesting-processing-infiltration that increased the percentage of survival of the graft obtaining positive similar results in its application all over the world. Nonetheless, publication of unlike results in the literature regarding survival of the graft with different percentages of reabsorption and the discovery of stem cells in the adipose tissue, have led to the accomplishment of numerous research works directed to understanding the biology of the fat graft, the role of the stem cells in this process and the influence that the different steps for transplantation have on the cellular viability. We present a review of the scientific literature about the theme to bring together the available information about these controversies.
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