Nematicidal Activity of Plant Extracts Against the Root-Knot Nematode, Meloidogyne incognita

The Open Natural Products Journal 10/2009; 2(1). DOI: 10.2174/1874848100902010077
Source: OAI


Nematicidal activity of extracts from plants was assayed against Meloidogyne incognita. In laboratory assays extracts from tobacco (Nicotiana tabacum L), clove (Syzygium aromaticum L), betelvine (Piper betle L), and sweet flag (Acorus calamus L) were most effective in killing the nematode, with an EC50 that was 5-10 times lower than the EC50 of the synthetic pesticides chlorpyrifos, carbosulfan and deltamethrin. The shapes of the dead nematodes differed in a characteristic way, and groups of pesticides and plant extracts could clearly be distinguished based on this phenomenon, which may be an indicator for the modes of action of the tested pesticides. In a greenhouse bioassay clove bud and betelvine were tested as mulch. Experiments revealed that the total number of live nematodes on roots of pepper plants treated with mulch of the clove bud was 7% of that of the controls and did not differ significantly from that of plants treated with the recommended synthetic pesticide carbofuran. The application of clove buds as a botanical pesticide for future use against nematodes is highly promising since clove is the 6th major plant grown on Bangka Island, and the market value of clove has decreased sharply over the last years

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    • "Different concentrations of n-hexane, DMSO and water extracts of M. afaqhusainii were studied against M. javanica. Hussey and Barker's method was used to isolate the eggs of M. javanica from Solanum melongena roots (Wiratno et al., 2009). The nematicidal activity of the algal extract was determined by a hatching test (Khalil & Badawy, 2012). "

    Full-text · Article · Jan 2016
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    • "The mechanisms of action of plant extracts may include denaturing and degrading of proteins, inhibition of enzymes and interfering with the electron flow in respiratory chain or with ADP phosphorylation (Elyousr et al., 2010; Taye et al., 2012). Extracts from leaves, seeds, flowers, stems, cloves, rhizomes, roots etc. of different plants reduce nematode population of Meloidogyne, Pratylenchus, Rotylenchulus, Helicotylenchus, Tylenchorhynchus, Radopholus, Tylenchulus, Hoplolaimus, Heterodera, Xiphinema etc. (Wiratno et al., 2009; Radhakrishnan, 2010; Taye et al., 2012; Akpheokhai et al., 2012). Botanicals reduce root gall formation, inhibit the hatching of juveniles and kill nematode (Akpheokhai et al., 2012). "

    Full-text · Article · Jun 2015
    • "40 mg/mL, respectively, against Meloidogyne spp. (Wiratno et al. 2009). The concentration-and time-dependent study of prodigiosin and copper sulphate revealed that the prodigiosin possessed high nematicidal potential than copper sulphate at very low concentration and in a very short time (Figure 1). "
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    ABSTRACT: Ineffectiveness of available nematicides and the high damage caused by plant-parasitic nematodes result in the urgent need to find some natural remedy for their control. Bioactivity of the pigment extracted from Serratia marcescens was screened for controlling nematodes at their juvenile stage. Test pigment was found effective against juvenile stages of Radopholus similis and Meloidogyne javanica at low concentrations (LC50 values, 83 and 79 μg/mL, respectively) as compared with positive control of copper sulphate (LC50 values, 380 and 280 μg/mL, respectively). The pigment also exhibited inhibition on nematode egg-hatching ability. Characterisation of extracted pigment with TLC, FTIR, HPLC, HPTLC and spectroscopic analysis confirmed the presence of prodigiosin as a bioactive metabolite. Considering the sensory mechanism of pathogen recognition by nematodes, the use of microbial secondary metabolites can be effective for nematode control rather than using whole organism.
    No preview · Article · Apr 2014 · Natural product research
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