Large-area spatially ordered arrays of gold nanoparticles directed by lithographically confined DNA origami. Nat Nanotechnol 5:121-126

Department of Nanoengineering, 9500 Gilman Drive M/C 0448, University of California San Diego, La Jolla, California 92093-0448, USA.
Nature Nanotechnology (Impact Factor: 34.05). 12/2009; 5(2):121-6. DOI: 10.1038/nnano.2009.450
Source: PubMed


The development of nanoscale electronic and photonic devices will require a combination of the high throughput of lithographic patterning and the high resolution and chemical precision afforded by self-assembly. However, the incorporation of nanomaterials with dimensions of less than 10 nm into functional devices has been hindered by the disparity between their size and the 100 nm feature sizes that can be routinely generated by lithography. Biomolecules offer a bridge between the two size regimes, with sub-10 nm dimensions, synthetic flexibility and a capability for self-recognition. Here, we report the directed assembly of 5-nm gold particles into large-area, spatially ordered, two-dimensional arrays through the site-selective deposition of mesoscopic DNA origami onto lithographically patterned substrates and the precise binding of gold nanocrystals to each DNA structure. We show organization with registry both within an individual DNA template and between components on neighbouring DNA origami, expanding the generality of this method towards many types of patterns and sizes.

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Available from: Luisa D Bozano, Dec 19, 2014
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    • "Numerous practices have since been made including the preparation of ordered nanostructures [9], gene and drug delivery [10] and biosensing [11]. For instance, DNA has been used as a rigid spacer between Au nanoparticles to prepare ordered architectures with distance-dependent optical [12] and physical properties [13] [14] in terms of heat, electron, and energy transfer. In Ref. [12], the authors reported the preparation of highly uniform DNAtailorable Au nanoparticle architectures with 1 nm inter-particle gap. "
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    ABSTRACT: In this paper, we reported the surface modification of multilayer carbon shell-encapsulated gold nanoparticles (Carbon Nanoparticles, or CNPs) via an oxygen plasma treatment approach and their subsequent functionalization with extraneous gold (Au) nanoparticles for Surface-enhanced Raman Scattering (SERS) sensing and DNA immobilization. The oxygen plasma treatment process led to purification of the multilayer carbon shell, reduction of the shell thickness, and derivatization of carbon shell with functional carboxylic groups. This further opens potential opportunities for surface functionalization of CNPs with extraneous Au nanoparticles or λ-DNA fragments, which were both achieved through a well-defined carbodiimide based covalent linking chemistry without removing the CNPs from the substrate. The resulting CNP–Au nanoparticle hybrids as well as the CNP-DNA architectures were characterized using various microscopic and spectroscopic techniques. The CNP–Au nanoparticle hybrids were further used as highly-sensitive SERS substrate for the detection of trace amount water-containing organic molecule. Meanwhile, they were also fundamentally simulated for their plasmonic behavior using discrete dipole approximation (DDA) method to understand the basic Raman enhancement principle. On the other hand, highly-ordered assembly of CNP-DNA architectures were also demonstrated in detail for their great potential in future DNA detection/recognition and bio-device applications.
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    • "Various approaches have been taken to control the placement of DNA nanostructures on surfaces. Electron-beam and nanoimprint lithography have been used to pattern hydrophilic regions in a hydrophobic matrix (on the substrate surface) to which DNA origami could physisorb [15] [16] [17]. Other strategies rely on non-covalent interactions, such as ionic attractions on carboxyl-functionalized Au surfaces [18], or physisorption on nanopatterned, and chemically modified, graphene films [19]. "
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    ABSTRACT: In order to exploit the outstanding physical properties of one-dimensional (1D) nanostructures such as carbon nanotubes and semiconducting nanowires and nanorods in future technological applications, it will be necessary to organize them on surfaces with precise control over both position and orientation. Here, we use a 1D rigid DNA motif as a model for studying directed assembly at the molecular scale to lithographically patterned nanodot anchors. By matching the inter-nanodot spacing to the length of the DNA nanostructure, we are able to achieve nearly 100% placement yield. By varying the length of single-stranded DNA linkers bound covalently to the nanodots, we are able to study the binding selectivity as a function of the strength of the binding interactions. We analyze the binding in terms of a thermodynamic model which provides insight into the bivalent nature of the binding, a scheme that has general applicability for the controlled assembly of a broad range of functional nanostructures.
    Full-text · Article · Jun 2013 · Nano Research
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    • "In another major step toward wafer scale origami applications, DNA origamies have been placed and oriented on lithographically patterned surfaces thus combining the top-down and bottom-up approaches [61]. Furthermore, Hung et al. reported specific positioning of gold nanoparticles on top of lithographically confined DNA origamies [62]. Positioning and alignment of origami between nanoelectrodes has been demonstrated using dielectrophoretic trapping [63]. "
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    ABSTRACT: The exploitation of DNA for the production of nanoscale architectures presents a young yet paradigm breaking approach, which addresses many of the barriers to the self-assembly of small molecules into highly-ordered nanostructures via construct addressability. There are two major methods to construct DNA nanostructures, and in the current review we will discuss the principles and some examples of applications of both the tile-based and DNA origami methods. The tile-based approach is an older method that provides a good tool to construct small and simple structures, usually with multiply repeated domains. In contrast, the origami method, at this time, would appear to be more appropriate for the construction of bigger, more sophisticated and exactly defined structures.
    Full-text · Article · Dec 2012 · International Journal of Molecular Sciences
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