Article

Photobacterium gaetbulicola sp. nov., a lipolytic bacterium isolated from a tidal flat sediment

Biotechnology Research Division, National Fisheries Research and Development Institute (NFRDI), Gijang, Busan, Republic of Korea.
International Journal of Systematic and Evolutionary Microbiology (Impact Factor: 2.51). 12/2009; 60(Pt 11):2587-91. DOI: 10.1099/ijs.0.016923-0
Source: PubMed

ABSTRACT

A Gram-negative, motile, non-spore-forming and lipolytic bacterial strain, designated Gung47(T), was isolated from a tidal flat on the west coast of Korea. Strain Gung47(T) grew optimally at 30 °C and with 2-5 % (w/v) NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain Gung47(T) belonged to the genus Photobacterium. Strain Gung47(T) exhibited 98.1 % 16S rRNA gene sequence similarity with Photobacterium rosenbergii LMG 22223(T) and 94.3-96.3 % similarity with other type strains of species of the genus Photobacterium. Strain Gung47(T) exhibited 47 % DNA-DNA relatedness to P. rosenbergii LMG 22223(T). Strain Gung47(T) contained Q-8 as the predominant ubiquinone and C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH, C(16 : 0) and C(18 : 1)ω7c as the major fatty acids. In this study, two closely related type strains, P. rosenbergii LMG 22223(T) and Photobacterium halotolerans LMG 22194(T), were also found to have Q-8 as the predominant ubiquinone. The DNA G+C content of strain Gung47(T) was 50.6 mol%. The differential phenotypic properties together with the phylogenetic and genetic distinctiveness of strain Gung47(T) demonstrated that this strain is distinguishable from recognized Photobacterium species. Therefore, strain Gung47(T) is considered to represent a novel species of the genus Photobacterium, for which the name Photobacterium gaetbulicola sp. nov. is proposed. The type strain is Gung47(T) (=KCTC 22804(T) =CCUG 58399(T)).

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    • "); 3, P. gaetbulicola Gung 47 T (Kim et al., 2010); 4, P. lutimaris LMG 25278 T (Chimetto et al., 2010; Jung et al., 2007); 5, P. jeanii LMG 25436 T (Chimetto et al., 2010; Srinivas et al., 2013); 6, P. leiognathi LMG 4228 T (Baumann & Baumann, 1984; Chimetto et al., 2010; Nogi, Masui & Kato, 1998; Yoshizawa et al., 2009). +, Positive; −, negative; w, weak; v, variable; nd, no data available. "
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    ABSTRACT: Five novel strains of Photobacterium (A-394T, A-373, A-379, A-397 and A-398) were isolated from bleached coral Madracis decactis (scleractinian) in the remote St Peter & St Archipelago (SPSPA), Mid-Atlantic Ridge, Brazil. Healthy M. decactis specimens were also surveyed, but no strains were related to them. The novel isolates formed a distinct lineage based on the 16S rRNA, recA, and rpoA gene sequences analysis. Their closest phylogenetic neighbours were Photobacterium rosenbergii, P. gaetbulicola, and P. lutimaris, sharing 96.6 to 95.8 % 16S rRNA gene sequence similarity. The novel species can be differentiated from the closest neighbours by several phenotypic and chemotaxonomic markers. It grows at pH 11, produces tryptophane deaminase, presents the fatty acid C18:0, but lacks C16:0 iso. The whole cell protein profile, based in MALDI-TOF MS, distinguished the strains of the novel species among each other and from the closest neighbors. In addition, we are releasing the whole genome sequence of the type strain. The name Photobacterium sanctipauli sp. nov. is proposed for this taxon. The G+C content of the type strain A-394T (=LMG 27910T = CAIM 1892T) is 48.2 mol%.
    Full-text · Article · May 2014 · PeerJ
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    • "The genus Photobacterium, which was first reported by Beijerinck (1889), belonging to the family Vibrionaceae of the class Gammaproteobacteria comprises a group of Gramstain-negative , facultatively aerobic, rod-shaped bacteria that require NaCl for growth. At the time of writing, the genus Photobacterium is composed of the following 23 species and 2 subspecies: Photobacterium aphoticum (Lucena et al., 2011), P. swingsii (Gomez-Gil et al., 2011), P. gaetbulicola (Kim et al., 2010), P. jeanii (Chimetto et al., 2010), P. aquimaris (Yoshizawa et al., 2009), P. kishitanii (Ast et al., 2007), P. lutimaris (Jung et al., 2007), P. ganghwense (Park et al., 2006), P. halotolerans (Rivas et al., 2006), P. lipolyticum (Yoon et al., 2005), P. rosenbergii (Thompson et al., 2005), P. frigidiphilum (Seo et al., 2005a), P. aplysiae (Seo et al., 2005b), P. indicum (Xie & Yokota, 2004), P. iliopiscarium (Urakawa et al., 1999), P. profundum (Nogi et al., 1998), P. damselae subsp. piscicida and P. damselae subsp. "
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    ABSTRACT: A Gram-staining-negative, motile, weakly halophilic and facultatively aerobic bacterium, designated strain YA11T, was isolated from tidal flat sediment at Yeongam bay, South Korea. Strain YA11T grew at 10-30 °C (optimum, 20 °C), at pH 6.0-10.0 (optimum, pH 6.5-7.5) and in the presence of 1-6 % (w/v) NaCl (optimum, 2-3 %). The major cellular fatty acids of the strain were summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c), summed feature 8 (comprising C18:1 ω7c and/or C18:1 ω6c) and C16:0. The DNA G+C content of the genomic DNA was 44.2 mol%. Strain YA11T contained Q-8 as the sole respiratory quinone. Phylogenetic tree based on 16S rRNA gene sequences showed that strain YA11Tformed a distinct phyletic lineage within the genus Photobacterium and the 16S rRNA gene sequence similarities between strain YA11T and the type strains of the genus Photobacterium ranged between 94.0 and 96.4 %. Based on the phenotypic, chemotaxonomic and molecular properties, strain YA11T represents a novel species of the genus Photobacterium, for which the name Photobacterium aestuarii sp. nov. is proposed, with strain YA11T(=KACC 16912T=JCM 18592T) as the type strain.
    Full-text · Article · Oct 2013 · International Journal of Systematic and Evolutionary Microbiology
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    • "bacterio.net/). Members of the genus Photobacterium have been isolated from marine water and sediment samples [such as Photobacterium aphoticum (Lucena et al., 2011), P. aquimaris (Yoshizawa et al., 2009), P. frigidiphilum (Seo et al., 2005a), P. gaetbulicola (Kim et al., 2010), P. ganghwense (Park et al., 2006), P. indicum (Xie & Yokota, 2004), P. lipolyticum (Yoon et al.,2005), P. lutimaris (Jung et al., 2007), P. angustum, P. profundum (Thyssen & Ollevier, 2005)], or marine animals [such as Photobacterium aplysiae (Seo et al., 2005b), P. kishitanii, (Ast et al., 2007), P. swingsii (Gomez-Gil et al., 2011), P. jeanii (Chimetto et al., 2010), P. rosenbergii (Thompson et al., 2005a), P. damselae, P. iliopiscarium, P. leiognathi, P. phosphoreum (Thyssen & Ollevier, 2005)]; an exception is Photobacterium halotolerans, which was isolated from a saline lake (Rivas et al., 2006). Some strains are pathogenic for aquatic animals and can therefore indirectly affect human health through their consumption, such as Photobacterium damselae subsp. "
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    ABSTRACT: A Gram-staining-negative, heterotrophic, facultatively anaerobic bacterium, designated AE6T, was isolated from a grouper (Epinephelus malabaricas) culture tank in a recirculating mariculture system located in Tianjin, China. Strain AE6T was able to grow at 15-40 oC (optimum, 30-35 oC), at pH 5.5-10.0 (optimum, pH 7.0-7.5) and in the presence of 0.5-7 % (w/v) NaCl (optimum, 2-3 %). It contained Q-8 as the predominant respiratory quinone, phosphatidylethanolamine (PE) and phosphatidylglycerol (PG) as the major polar lipids, and C16:1ω7c/C16:1ω6c (40.4 %), C18:1ω7c (15.5 %) and C16:0 (13.5 %) as the predominant cellular fatty acids. The genome DNA G+C content was 47.1 mol %. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AE6T belonged to the genus Photobacterium (94.2-96.8 % of 16S rRNA gene sequence similarity) and formed a distinct phylogenetic lineage within the genus and exhibited the highest sequence similarities to P. aphoticum CECT 7614T (96.8 %). Multilocus sequence analysis (MLSA) using four loci (gyrB, rpoA, pyrH and recA gene) also revealed that strain AE6T was phylogenetic related to the genus Photobacterium. Based on the phylogenetic, chemotaxonomic and phenotypic evidences, strain AE6T is considered to represent a novel species of the genus Photobacterium, for which the name Photobacterium aquae sp. nov. is proposed. The type strain is AE6T (=CGMCC 1.12159T = JCM 18480T).
    Full-text · Article · Oct 2013 · International Journal of Systematic and Evolutionary Microbiology
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