Identification of an Arabidopsis Nodulin-related protein in heat stress

Key Laboratory of Tropical Forest Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, 650223, People's Republic of China.
Moleculer Cells (Impact Factor: 2.09). 12/2009; 29(1):77-84. DOI: 10.1007/s10059-010-0005-3
Source: PubMed


We identified a Nodulin-related protein 1 (NRP1) encoded by At2g03440, which was previously reported to be RPS2 interacting protein in yeast-two-hybrid assay. Northern blotting showed that AtNRP1 expression was suppressed by heat stress (42 degrees C) and induced by low temperature (4 degrees C) treatment. Strong GUS staining was observed in the sites of meristematic tissues of pAtNRP1:: GUS transgenic plants, such as shoot apex and root tips, young leaf veins, stamens and stigmas of flowers, and abscission layers of young siliques. To study AtNRP1 biological functions, we have characterized both loss-of-function T-DNA insertion and transgenic overexpression plants for AtNRP1 in Arabidopsis. The T-DNA insertion mutants displayed no obvious difference as compared to wild-type Arabidopsis under heat stress, but the significant enhanced susceptibility to heat stress was revealed in two independent AtNRP1-overexpressing transgenic lines. Further study found that the decreased thermotolerance in AtNRP1-overexpressing lines accompanied significantly decreased accumulation of ABA after heat treatment, which was probably due to AtNRP1 playing a role in negative-feedback regulation of the ABA synthesis pathway. These results support the viewpoint that the application of ABA inhibits nodulation and nodulin-related gene expression and threaten adverse ambient temperature can impact the nodulin-related gene expression.

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    • "A gene encoding nodulinrelated protein was also found in the CR locus. There is some evidence that a nodulin-related gene was induced and provided a protective defense response when Arabidopsis roots were colonized by several strains of Pseudomonas thivervalensis (Cartieaux et al. 2003), and that this gene plays a role in negative feedback regulation of the ABA-synthesis pathway (Fu et al. 2010). An increase in ABA was observed in Brassica roots during development of clubroot disease (Devos et al. 2005), and was thought to be a signal for protecting seriously clubbed roots from drought (Siemens et al. 2006). "
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    • "Nodulin-related proteins were also reported to play a role in pathogen and heat stress tolerance in A. thaliana (Fu et al. 2010) as well as they may be markers of Rhisobium infection in Vicia faba (Becker et al. 2001). The third gene, a transcript highly similar to dinelactone hydrolase protein family, was predicted to be targeted by conserved miR159c. "
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    • "For example, Nodulin-related protein 1 (NRP1) is likely to be involved in the negativefeedback regulation of ABA biosynthesis. Transgenic plants overexpressing NRP1 have reduced ABA accumulation and increased susceptibility to heat stress (Fu et al. 2010). The non-protein amino acid b-aminobutyric acid (BABA) is a xenobiotic compound that can be transported to all the aerial parts of Arabidopsis when watered to the roots (Zimmerli et al. 2008). "
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