Microfluidic in vivo Screen Identifies Compounds Enhancing Neuronal Regeneration

Research Laboratory of Electronics, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Conference proceedings: ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference 01/2009; 2009:5950-2. DOI: 10.1109/IEMBS.2009.5334771
Source: PubMed


Compound screening is a powerful tool to identify new therapeutic targets, drug leads, and elucidate the fundamental mechanisms of biological processes. We report here the results of the first in vivo small-molecule screens for compounds enhancing neuronal regeneration. These screens are enabled by the microfluidic devices we have developed for C. elegans. The devices enable rapid and repeatable animal immobilization which allows high-throughput and precise surgery. Following surgery, animals are exposed to the contents of a small-molecule library and assayed for neuronal regeneration. Using this screening method we have identified several compounds that enhance neural regeneration in vivo.

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Available from: Chrysanthi Samara, Aug 16, 2014
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