Non-Ionizing Radiation with Nanosecond Pulsed Electric Fields as a Cancer Treatment: In Vitro Studies

Eastern Virginia Medical School and Frank Reidy Research Center for Bioelectrics, Old Dominion University, USA.
Conference proceedings: ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Conference 01/2009; 2009:6509-12. DOI: 10.1109/IEMBS.2009.5333139
Source: PubMed


Cancer continues to be a major risk to the health and well being among populations around the world. A new method using ion-ionizing radiation with nanosecond pulsed electric fields (nsPEFs) provides a novel means to treat cancer at local sites. NsPEFs promote cell death in several cell types and here we investigated mechanisms for cell death induction. In murine B16f10 melanoma, murine E4 squamous carcinoma, murine Hep1-6 and human HepG2 hepatocellular carcinoma, nsPEFs induced cell death in 90-95% of cells. Cell death coincides with decreases in the mitochondria membrane potential, increases in YO-PRO-1 uptake and active caspases in the presence or absence of cytochrome c release. The results indicate that nsPEFs induced cell death by multiple apoptosis mechanisms that involve mitochondrial responses, but not necessarily through cytochrome c release. Further, these in vitro studies suggest a potential to induce cell death that bypasses cancer mechanisms that evade apoptosis.

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Available from: Stephen Beebe, Dec 18, 2015
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    • "New research has focused on decreasing the treatment time to nanosecond levels in order to decrease the side effects of PEF on a food's main structure. Nano-level application of PEF is being used for cancer and tumor therapy in human cells (Zygulska and Pawlega, 2008; Beebe et al., 2009; Donthula et al., 2009; Mi et al., 2009a, 2009b; Yang et al., 2009). Katsuki and others (2002) applied PEF in ultra short pulses (nanosecond PEF) and set the electric field amplitude to 130 kV/s and pulse width to 45 ns. "
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