International Journal of Food Sciences and Nutrition,
February 2010; 61(1): 1–10
Stevia (Stevia rebaudiana) a bio-sweetener: a review
S. K. GOYAL
& R. K. GOYAL
Department of Agricultural Engineering and Food Technology, S.V.B.P. University of Agriculture
& Technology, Meerut, India, and
Department of Animal Husbandry & Dairying, RBS College,
Studies revealed that Stevia has been used throughout the world since ancient times for various
purposes; for example, as a sweetener and a medicine. We conducted a systematic literature
review to summarize and quantify the past and current evidence for Stevia. We searched relevant
papers up to 2007 in various databases. As we know that the leaves of Stevia plants have
functional and sensory properties superior to those of many other high-potency sweeteners,
Stevia is likely to become a major source of high-potency sweetener for the growing natural food
market in the future. Although Stevia can be helpful to anyone, there are certain groups who are
more likely to beneﬁt from its remarkable sweetening potential. These include diabetic patients,
those interested in decreasing caloric intake, and children. Stevia is a small perennial shrub that
has been used for centuries as a bio-sweetener and for other medicinal uses such as to lower blood
sugar. Its white crystalline compound (stevioside) is the natural herbal sweetener with no calories
and is over 100300 times sweeter than table sugar.
Keywords: Stevia leaves, rebaudioside, stevioside, extract, powder, medicinal use
Stevia rebaudiana is a small perennial growing up to 6580 cm tall, with sessile,
oppositely arranged leaves. Different species of Stevia contain several potential sweet-
ening compounds, with S. rebaudiana being the sweetest of all. Stevia is a semi-humid
subtropical plant that can be grown easily like any other vegetable crop even in the
kitchen garden. The soil should be in the pH range 6.57.5; well-drained red soil and
sandy loam soil. Saline soils should be avoided to cultivate this plant. Stevia has been
successfully cultivated in recent years in many areas of Indian states: Rajasthan,
Maharashtra, Kerela and Orissa. The increasing demands for natural sweeteners
have driven the farmers in India toward large-scale Stevia cultivation. Diterpene
glycosides are the group of natural sweeteners that have been extracted from Stevia.
The leaves of wild Stevia plants contain 0.3% dulcoside, 0.6% rebaudioside C, 3.8%
rebaudioside A and 9.1% stevioside.
Stevia (Asteraceae) is a woody shrub that can reach 80 cm in height when it is fully
matured. The Stevia genus comprises at least 110 species (Rajbhandari and Roberts
1983) but there may be as many as 300. Its habitat extends from the southwestern
United States to the Brazilian highlands (Soejarto et al. 1982). Different species of
Correspondence: S. K. Goyal, Department of Agricultural Engineering and Food Technology, S.V.B.P. University of
Agriculture & Technology, Meerut 250 110, India. E-mail: email@example.com
ISSN 0963-7486 print/ISSN 1465-3478 online 2010 Informa UK Ltd
Stevia contain several potential sweetening compounds, with S. rebaudiana Bertoni
being the sweetest of all (Soejarto et al. 1982; Kinghorn et al. 1984) The use of
S. rebaudiana as a sweetener can be found in many parts of Central and South America,
where this species is indigenous (Melis 1992), as well as in Japan (Kinghorn et al.
1984). People in Japan have been using Stevia as a sweetener in products such as
seafood, soft drinks, and candies (Soejarto et al. 1982). This plant has been used in
several areas of the world, such as in Brazil and Paraguay, as a natural control for
diabetes (Jeppesen et al. 2000). Stevia also has been used to help control weight in
obese persons (Suttajit et al. 1993).
The complete chemical composition of Stevia species is not yet available. However, a
variety of Stevia species has been tested for their chemical compositions. The useful
part of this shrub is the leaves. Out of 110 species tested for sweetness, only 18 were
found to possess this characteristic (Soejarto et al. 1982). Eight ent-kaurene glyco-
sides—namely dulcoside A, rebaudiosides AE, steviolbioside, and stevioside—
produce the sweet taste sensation (Kinghorn et al. 1984). These glycosides are mainly
compounds of the diterpene derivative steviol (Shibata et al. 1995). S. rebaudiana
Bertoni, the sweetest species, contains in its leaves all of the eight ent-kaurene glycosides
(Kinghorn et al. 1984), with stevioside being the major constituent (38% by weight of
the dried leaves) (Melis 1992). In addition, S. rebaudiana Bertoni contains stigmasterol,
b-sitosterol, and campesterol (D’Agostino et al. 1984). The same species also contains
steviol, a product formed by enzymatic hydroxylation within the plant (Kim et al. 1996).
Other chemicals with no sweet taste are also found in Stevia species and some may even
be bitter in taste. Stevisalioside A (from the roots of Stevia salicifolia) (Mata et al. 1992),
longipinane derivatives in the roots of Stevia connata (Sanchez-Arreola et al. 2000),
epoxylabdane diterpenes and a clerodane derivative in the leaves of Stevia subpubescens
(Roman et al. 2000), ﬂavonoids from the leaves of S. rebaudiana (Soejarto et al. 1982),
Stevia nepetifolia (Rajbhandari and Roberts 1983), Stevia microchaeta,Stevia monardifolia,
Stevia origanoides (Rajbhandari and Roberts 1985) and Stevia procumbens (aerial parts)
(Sosa et al. 1985), and sesquiterpene lactones from the aerial parts of S. procumbens and
the leaves of S. origanoides (Calderon et al. 1987) are in this group.
Proximate composition of Stevia
Six sweet-tasting compounds have been reported in the leaves of S. rebaudiana
Bertoni—stevioside, rebaudiosides A, D and E, dulcosides A and B (Kohda et al.
1976; Kobayashi et al. 1977). Stevioside is a glycoside with a glucosyl and sophorosyl
residue attached to the aglycon steviol; the latter has a cyclo-pentanoperhydrophenan-
threne skeleton. The C4 and C13 of steviol are connected to the b-glucosyl and b-
sophorosyl group, respectively. The structure of rebaudioside A is the same as that of
stevioside except that the sophorosyl residue is replaced by a glucosyl-(13)-sophorosyl
residue. The Stevia sweeteners are similar in structure, in that a steviol aglycon is
connected at C4 and C13 to trisaccharides consisting of glucose and/or rhamnose
residues (Kobayashi et al. 1977). Stevioside is a natural sweetener extracted from leaves
of Stevia (Genus Jan 2003).
Soejarto et al. (1983) believed that the sesquiterpene lactones are responsible for the
bitter aftertaste. Phillips (1987) described a European patent held by the Stevia
2S. K. Goyal et al.
Company, which attributes the bitter aftertaste to the presence of essential oils, tannins,
and ﬂavonoids. Nevertheless, as pointed out, stevioside and rebaudioside A are partially
responsible for the aftertaste, even though the contribution of rebaudioside A is signif-
icantly less than that of stevioside. The S. rebaudiana Bertoni contains a complex mixture
of labdane diterpenes, triterpenes, stigmasterol, tannins, volatile oils, and eight diterpe-
nenic glycosides: stevioside, steviobioside, dulcoside, and rebaudiosides A, B, C, D,
and E. The most abundant substances are stevioside and rebaudioside A. Of the Stevia
glycosides, rebaudioside A is the sweetest and the most stable, and it is less bitter than
stevioside. Rebaudioside E is as sweet as stevioside, and rebaudioside D is as sweet as
rebaudioside A, while the other glycosides are less sweet than stevioside (Cramer and
Ikan 1987). According to Pederson (1987), stevioside is a white, crystalline powder
extracted from the leaves of the Stevia plant. Its chemical identiﬁcation and quantitative
compositions are listed for those with a more scientiﬁc interest in the product. It is 100%
natural, having no (zero) calories, is 200300 times sweeter than sugar, heat stable to
198C, non-fermentable, a ﬂavour enhancer, and is anti-plaque and anti-caries. He
reported the proximate composition of S. rebaudiana Bertoni that is presented in Table I,
and compared Stevia leaf powder and Stevia white extract with granulated sugar
Ngowatana (1997) puriﬁed the Stevia extract and obtained stevioside and its
products that were a white ﬁne powder and highly hygroscopic. It must be kept in
an air-tight package to prevent moisture absorption. In large-scale production the same
methods are used, except for the ﬁnal step that produced dry products by using a spray
dryer. Researchers reported that 3,000 g Stevia could produce 101.56 g light-yellow
ﬁne powder of stevioside and its products (Table III). Product compositions were
stevioside. The amounts of iron and calcium were 0.97 and 1.47 mg/g product,
respectively. The moisture content of the product was 9.31%.
Table I. Proximate composition of S. rebaudiana Bertoni.
Sample number Constituent Value (%)
1 Aluminium 0.0072
2 Manganese 0.0147
3 Ash 6.3000
4 Phosphorus 0.3180
6 Potassium 1.7800
7 Calcium 0.5440
8 Protein 11.200
9 Chromium 0.0039
10 Selenium 0.0025
11 Cobalt 0.0025
12 Silicon 0.0132
13 Fat 1.9000
14 Sodium 0.0892
15 Fibre 15.200
16 Tin 0.0015
17 Iron 0.0039
18 Vitamin 0.0110
19 Magnesium 0.3490
20 Water 82.300
Stevia a bio-sweetener 3
According to Sharma et al. (2006), the fresh Stevia leaves contain a large amount of
water between 80 and 85%. The main constituents present were glycosides such as
stevioside, steviol and rebaudioside A and B. The other constituents present in Stevia
were ascorbic acid, b-carotene, chromium, cobalt, magnesium, iron, potassium,
phosphorous, riboﬂavin, thiamin, tin, zinc, and so forth. The other chemicals found
in Stevia include apigenin, austroinulin, avicularin, b-sitosterol, caffeic acid, compes-
terol, caryophyllene, centaureidin, chorogenic acid, chlorophyll, cynaroside, daucos-
terol, di-terpene glycoside, dulcosides A and B, foeniculin, formic acid, gibberellic
acid, gibberellin, indole-3-acetonitrile, isoquercitrin, isosteviol, kaempferol, kaurene,
lupeol, luteolin, polysatachoside, quercetin, quercitrin, scooletin, stigmasterol, umbel-
liferone and xanthophyllus.
Physiological and pharmacological actions
Stevia is used in many parts of the world as a non-caloric sweetener (Matsui et al.
1996). Along with sweetness, a bitter taste is also felt in humans (Jakinovich and Moon
1990). As an extract, this herb was found to have similar potency with regard to
sweetness as a 10% sucrose solution at either pH 3.0 or 7.0. The same study also
showed that the herbal extract had similar potency to that of aspartame and a
cyclamate/saccharin mixture (Cardello et al. 1999). The potency of Stevia extracts
was found to be higher than other herbal sweet extracts such as those of Thladiantha
grosvernorii (Cucurbitaceae) or Abrus precatorius (Fabaceae). The sweetness of stevio-
side, the major sweet component in Stevia species, was detected in a concentration as
low as 24 mg/ml (Jakinovich and Moon 1990).
Table II. Comparison of Stevia leaf powder and Stevia white extract with granulated sugar.
Granulated sugar Stevia leaf powder Stevia white extract
1 teaspoon 1/8 teaspoon Dust on spoon
1 tablespoon 3/8 teaspoon 1/2 pinch
1/4 cup 1/2 teaspoon Pinch
1/2 cup 1 tablespoon 1/8 teaspoon
1 cup 2 tablespoons 1/4 teaspoon
3.75 pounds 7.2 ounces 0.3 ounces
10 pounds 19.2 ounces 0.8 ounces
Table III. Some commercial available Stevia products in the USA.
Product Manufacturer Type
Stevia At Stevia LLC (Valley Forge, PA, USA) Crystals
Stevia extract Life Extension Foundation (Fort Lauderdale,
JAJ Stevioside JAJ Group, Inc. (Jacksonville, FL, USA) Powder
Stevia Liquid Extract Baar Products, Inc. (Downingtown, PA, USA) Liquid
Stevia Dark Liquid
Stevia NOW (Shrub Oak, NY, USA) Liquid concentrate
Stevia Pure Powder Extract Stevia NOW Powder extract
Stevia Tablet Stevia NOW Tablets (100400 mg)
4S. K. Goyal et al.
Despite centuries of use, there is still a lack of comprehensive clinical studies on Stevia
as a supplement. In normal human volunteers, the effect of administering extracts of
S. rebaudiana on glucose tolerance tests was investigated. Subjects were given aqueous
extracts from 5 g leaves every 6 h for 3 days. A glucose tolerance test was performed
before and after administration of the extracts. The results showed that treatment with
Stevia resulted in an increase in glucose tolerance and a decrease in plasma glucose
concentrations (Curi et al. 1986). Moreover, it was shown recently that both steviol and
stevioside can produce a direct effect on beta cells in the pancreas to release insulin.
The authors concluded that this plant may have a potential use in the management of
type 2 diabetes (Jeppesen et al. 2000).
Cariogenic and mutagenic effects
Since Stevia products are used as sugar substitutes by many populations, a study was
conducted to test whether stevioside and rebaudioside A may have the potential of
causing dental caries from prolonged use. Rats were fed a diet containing 0.5%
stevioside or 0.5% rebaudioside A for 5 weeks. Neither compound showed a potential
of increasing the risk of developing dental caries (Das et al. 1992). Several researchers
investigated the risk of mutagenicity. In two studies (Matsui et al. 1986; Pezzuto et al.
1996), steviol produced a dose-related positive mutagenic effect in some tests. In the
same studies, stevioside was found to be devoid of this effect. Other reports indicated
lack of mutagenicity of both compounds (Suttajit et al. 1993; Klongpanichpak et al.
1997). Because of these contradictory reports, the Food and Drug Administration is
still cautious in introducing this herb as a sugar substitute until its safety is completely
established (FDA 1999).
Some examples of Stevia products available on the market in the USA are presented
in Table III. Products of Stevia can be purchased directly from various companies or
from local pharmacies. Many companies sell Stevia products via the Internet.
Studies on food safety, including an extensive review of the literature, undertaken prior
to 1982 (Lee 1979; Kinghorn 1982) concluded that Stevia leaves and extracts are safe;
studies since then conﬁrm this. Possible medicinal uses have been investigated often by
using Stevia extracts as intravenous infusions in rats; possible effects on glucose
metabolism, diuresis, organ weights, endocrine function, and so on, have been studied
in this way (Kinghorn 1987; Nunes and Pereira 1988; Oliveira Filho 1988; Suanar-
unsawat and Chaiyabut 1996, 1997). Stevia extract infusions have also shown some
anti-androgenic activity in rats (Sincholle and Marcorelles 1989). Likely beneﬁcial
effects of Stevia extracts, as antioxidants and to relieve blood pressure and hyperten-
sion, have also been shown (Chan et al. 1998; Xi 1998; Xi et al. 1998). Steviol (a
precursor in the biosynthesis of steviosides) can be produced from steviosides exper-
imentally using speciﬁc bacteria but not in situ in the human body. Steviol can exhibit
some toxic and mutagenic activity (Tateo 1990).
Stevia a bio-sweetener 5
Investigations of the effect of aqueous extract of S. rebaudiana leaves on glucose
tolerance have been carried out by Curi et al. (1986) on volunteers. Aqueous extract
of 5 g leaves were administered to volunteers at regular 6-hourly intervals for 3 days,
with glucose tolerance tests performed before and after extract administration.
The extract increased glucose tolerance; it signiﬁcantly decreased plasma glucose
levels during the test and after overnight fasting in all volunteers. In Japan, where
artiﬁcial chemical sweeteners are not approved, many toxicology safety studies
have been conducted (Elton Johnson 1990). Among studies carried out are some
to investigate carcinogenicity and mutagenicity (if any) in animal testing (Oliveira
Filho 1988; Toruan-Mathius et al. 1995; Toyoda 1997), to show dental beneﬁts in
the form of plaque inhibition and cavity reduction (Elton-Johnson 1990), to conﬁrm
the safety of Stevia for diabetic use (Polyanskii et al. 1997; Thamolwan and
Narongsak 1997). The safety of feeding to animals, chickens and humans has also
been conﬁrmed by a wide range of studies (Sincholle and Marcorelles 1989; Smolyar
1993; White et al. 1994; Melis 1995, 1997; Suanarunsawat and Chaiyabut 1996,
1997; Wood 1996; Polyanskii et al. 1997).
The traditional method of use by the Paraguayan Guarani Indians was to dry
the leaves and to use them to sweeten tea and medicines or to chew the leaves as a
‘sweet treat’. Stevia was regularly used in drinks many times a day, not just
occasionally, with no side effects. The use of dried leaves (pieces or powdered) is
unacceptable in domestic cooking and does leave a sediment in clear drinks, and so
forth, and can also leave a green colour. There may also be an unpleasant aroma
associated with the dried leaves. Appropriate processing of the dry herbage may
remove this aroma, which is due to speciﬁc leaf compounds (not steviosides)
(Tsanava et al. 1991). Although Stevia has been used without any problems for
many years in its native Paraguay and in other countries for lesser periods, health
and safety issues have been receiving considerable attention in the past 20 years.
There has been considerable media attention in the USA, including claims and
counterclaims before the US FDA. Many of these claims relate to its potential
competitive position in relation to aspartame. Stevia products have been approved
for use in the USA as nutrition supplements although many protagonists claim it
should be granted Generally Regarded As Safe status in the same manner as tea,
coffee, sugar and fruit and vegetables, and so on. The general safety of steviosides
could be largely due to the fact that they are not broken down nor are absorbed in the
digestive tract (Hutapea 1997). Bacteriological studies on hot water extract from
S. rebaudiana have been carried out by Tomita (1997). Lactobacilli were not killed on
exposure to the fermented extract; however, under acidic conditions, the extract was
found to be bactericidal.
In Japan, artiﬁcial sweeteners were banned some 40 years ago so Stevia has been
their chosen alternative to sweeten their food and beverages. The Japanese have
performed over 40,000 clinical studies and found Stevia to be safe. Stevia in its raw
form, although incredibly sweet, has a very subtle liquorice essence to it. A sign of an
excellent Stevia product is one that is free of this liquorice essence and still not bitter
(Tateo et al. 1998). Genus Jan (2002) concluded that Stevia and stevioside are safe
when used as a sweetener. Stevia is suited for both diabetics and Phenylketonuria
(PKU) patients, as well as for basepersons intending to lose weight by avoiding sugar
supplements in the diet. No allergic reactions to it seem to exist. Midmore and Rank
(2002) found that the aqueous extracts of the leaves—boiled in water, cooled, then
6S. K. Goyal et al.
strained (ﬁltered)—are preferred in many situations and are better suited for controlled
levels of sweetening. Crystalline powders and extracts are preferred in commercial
situations as they have a ﬁxed known sweetening value. Fixed concentration liquids are
also acceptable. Kumar et al. (2007) reported that the Stevia is sweetest plant in the
world because leaves contain diterpene glycoside that has a sweet taste but it is not
metabolized and contains no calories. It is native to a relatively small area of eastern
Paraguay (on the Brazilian border) where its leaves have been used by the local Guarani
Indians as a sweetener for many hundreds of years. They specially used it in the local
green tea (Mate tea-Hex sp.), as well as with other unpalatable medicinal and other
drinks. The leaves are 30 times sweeter than cane sugar and can be safely used by
diabetic patients. Sharma and Mogre (2007) observed the effect of consumption of
Stevia extract on 20 selected hypercholestronic women: 20 ml extract was used to
intervene in one subject in a glass of water (200 ml). They found the consumption of
Stevia extract reduces the levels of cholesterol, triglyceride and low-density lipoprotein-
cholesterol signiﬁcantly while an increase in high-density lipoprotein-cholesterol was
noted, which is desirable. They concluded that Stevia extract had a hypolipidaemic
effect used to reduce the resistance of cardiovascular disease. The documented
properties of Stevia are anti-bacterial, anti-fungal, anti-inﬂammatory, anti-microbial,
anti-viral, anti-yeast, cardio-tonic, diuretic, hypoglycaemic, hypotensive and as a
vasodilator. Stevia has an advantage over artiﬁcial sweeteners because it is stable at
high temperatures and has a pH range 39. Stevia extract is used as a sweetener or
ﬂavour enhancer in many countries such as China, Japan, Korea, Israel, Brazil and
Paraguay. It is also used in soft drinks, ice creams, cookies, pickles, chewing gum, tea
and skincare products (Lee 1979; Kinghorn 1982, 1987; Elton Johnson 1990; Tateo
1990). Stevia plant and its extract both are used in weight-loss programmes because of
their ability to reduce the craving for sweet and fatty foods (Jain et al. 2007).
Uses of Stevia
.Stevia is safe for diabetics, as it does not affect blood sugar levels.
.Stevia does not have the neurological or renal side effects as other artiﬁcial
.Stevia possess anti-fungal and anti-bacterial properties in addition to its other
versatile uses. It can be safely used in herbal medicines, tonics for diabetic patients
and also in daily usage products such as mouthwashes and toothpastes.
.Mild Stevia leaf tea offers excellent relief for an upset stomach.
Stevia is a herb that is used extensively in various areas of the world (without
documentation of long-term use and effects) as a non-caloric sugar substitute. Various
reports in animals and humans indicate that the safety of this herb is not yet completely
determined. The current status of using this herb in the USA is as a ‘dietary
supplement’. Until further information is available, pharmacists should be advised
to conform to the FDA recommendation when counselling patients about this herb.
Speciﬁcally, mild to moderate use as a supplement should be safe, but increased use for
other pharmacological effects may not be warranted.
Stevia a bio-sweetener 7
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