Development of a new method for improved identification and relative quantification of unknown metabolites in complex samples: Determination of a triterpenoid metabolic fingerprint for the in situ characterization of Ganoderma bioactive compounds

Bindley Bioscience Center, Purdue University, West Lafayette, IN 47907, USA.
Journal of Separation Science (Impact Factor: 2.74). 12/2009; 32(23-24):4052-8. DOI: 10.1002/jssc.200900496
Source: PubMed


Ganoderma lucidum is a mushroom with a long history of medical applications. Research has demonstrated chemotherapeutic effects of G. lucidum in tissue culture, and bioactive fractions of the mushroom have been shown to contain high levels of triterpenoids and polysaccharides. In this study, we developed a new method for the detection of ganoderic acids and other triterpenes in Ganoderma mushroom extracts based on a post-biosynthetic stable isotope encoding technique. Overall, 57 doublets were identified as potential ganoderic acids and 11 of those matched with the database. Ganoderic acid A, F and H were confirmed by standards and their absolute concentrations were measured in GLT (GA A: 3.88 mg/g; GA F: 0.95 mg/g and GA H: 1.74 mg/g) and ReishiMax (GA A: 2.32 mg/g; GA F: 0.43 mg/g and GA H: 0.85 mg/g) extracts. The method was also used for the evaluation of bioavailability of triterpenes after an oral application and demonstrated the presence of G. lucidum triterpenes in plasma.

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Available from: Daniel Sliva, Dec 11, 2014
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    • "A simple, rapid, and accurate HPLC method allowing the quantification of GA in different kinds of Lingzhi and their products was developed and validated (Huie and Di 2004; Tang et al. 2006a, b; Wang et al. 2007a, b, c; Keypour et al. 2010). So setting up the standard fingerprint and analysis methods of Lingzhi, and controlling the Lingzhi and its product quality have attracted attention in the academic circles worldwide (Gu and Weng 2008; Ding et al. 2009a, b; Adamec et al. 2009). Determination of triterpenoid content usually uses the GA-B as the standard. "
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    • "Moreover, we have previously identified some of the triterpenes in RM (e.g. ganoderic acids A, F, H, Mh, S, lucidenic acid B, D, D1, E1, L and methyl lucidenate G) [77], that can be used for the standardization of the active supplements or G. lucidum extracts. Our study is with agreement with previous study demonstrating stimulation of glucose uptake and activation of AMPK in rat muscle cells [78]. "
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