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GC–MS Analysis of Polysaccharides from an Intergeneric Hybrid of Pleurotus florida and Cordyceps militaris: A Comparative Study

Authors:
Varsha Meshram at Pandit Ravishankar Shukla University
Varsha Meshram
Prachi Thakur
Prachi Thakur
Prachi Thakur
  • This person is not on ResearchGate, or hasn't claimed this research yet.
Shailesh Kumar Jadhav
Shailesh Kumar Jadhav
Shailesh Kumar Jadhav
  • This person is not on ResearchGate, or hasn't claimed this research yet.
Nagendra Kumar Chandrawanshi Ph.D. at Pandit Ravishankar Shukla University
Nagendra Kumar Chandrawanshi Ph.D.
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Abstract and Figures

Edible and medicinal mushrooms are valuable sources of polysaccharides, known for their dual roles as immunostimulants and immunosuppressants. This study aimed to enhance polysaccharide content by fusing two mushroom species, P. florida and C. militaris, while exploring their antioxidant and antibacterial potential. These mushrooms have diverse health benefits, including lowering high cholesterol, providing anti-inflammatory effects, supporting diabetes management, aiding in cancer treatment, and enhancing the efficacy of COVID-19 vaccines. Successful hyphal fusion was achieved, and optimal culture conditions were determined using response surface methodology. The hybrids exhibited superior growth compared to the parental strains. Hyphal fusion improved several attributes, resulting in diverse hybrids with increased biomass and metabolite production. FTIR analysis confirmed the presence of exopolysaccharides, with concentrations measured at 28.4 g/L (P1), 31.50 g/L (CD), and 36.74 g/L (F3). GC–MS analysis identified various bioactive metabolites, including a higher concentration of dimethyl palmitamine in the hybrid, a novel compound, butanenitrile, 2-(methoxymethoxy), which was not found in the parental strains. These compounds are likely responsible for the enhanced antimicrobial and antioxidant activities. Graphical Abstract
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Vol.:(0123456789)
Applied Biochemistry and Biotechnology (2025) 197:1805–1830
https://doi.org/10.1007/s12010-024-05121-z
ORIGINAL ARTICLE
GC–MS Analysis ofPolysaccharides fromanIntergeneric
Hybrid ofPleurotus florida andCordyceps militaris:
AComparative Study
VarshaMeshram1· PrachiThakur2· ShaileshKumarJadhav1·
NagendraKumarChandrawanshi1
Accepted: 19 November 2024 / Published online: 29 November 2024
© The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2024
Abstract
Edible and medicinal mushrooms are valuable sources of polysaccharides, known for their
dual roles as immunostimulants and immunosuppressants. This study aimed to enhance
polysaccharide content by fusing two mushroom species, P. florida and C. militaris, while
exploring their antioxidant and antibacterial potential. These mushrooms have diverse
health benefits, including lowering high cholesterol, providing anti-inflammatory effects,
supporting diabetes management, aiding in cancer treatment, and enhancing the efficacy
of COVID-19 vaccines. Successful hyphal fusion was achieved, and optimal culture condi-
tions were determined using response surface methodology. The hybrids exhibited superior
growth compared to the parental strains. Hyphal fusion improved several attributes, result-
ing in diverse hybrids with increased biomass and metabolite production. FTIR analysis
confirmed the presence of exopolysaccharides, with concentrations measured at 28.4g/L
(P1), 31.50 g/L (CD), and 36.74 g/L (F3). GC–MS analysis identified various bioactive
metabolites, including a higher concentration of dimethyl palmitamine in the hybrid, a
novel compound, butanenitrile, 2-(methoxymethoxy), which was not found in the parental
strains. These compounds are likely responsible for the enhanced antimicrobial and anti-
oxidant activities.
Graphical Abstract
Extended author information available on the last page of the article
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