The regulation of self-control conflicts is integral to exerting self-control and pursuing (long-term) goals. Nonetheless, prevailing conceptualizations of self-control conflict remain vague, and the mechanisms and boundary conditions through which self-control conflict emerges are rarely empirically tested. In the present research, we thus propose that self-control conflicts originate in accessible ambivalent attitudes. To examine our attitudinal perspective on self-control and self-regulation, we investigated how (ambivalent) attitudes influence self-control conflicts and how resolving these attitudinal origins may enhance self-control and avert future conflicts. We ran a 21-day diary study assessing daily inhibition conflicts about eating meat among conflicted vegetarians (N = 156, k = 2,346). Our findings suggest that holding (positive) attitudes that conflict with predominant (negative) attitudes predicted heightened conflict frequency in people’s daily lives, and the situational accessibility of both positive and negative attitudes is associated with conflict magnitude. Moreover, to cope with these conflicts, people often engaged in attitude-based self-regulation involving the affirmation of negative and the disaffirmation of positive attitudes toward eating meat, thereby successfully exerting self-control. Contrary to our prediction, however, we did not find evidence for the effectiveness of attitude-based self-regulatory strategies for mitigating subsequent conflict. In fact, various self-regulatory strategies, including the disaffirmation of positive attitudes, self-distraction, and thought suppression, even escalated subsequent conflict. These findings suggest that our attitudinal perspective on self-control and self-regulation provides a parsimonious and testable conceptualization of self-control conflicts.