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Marine cosmetics and the blue bioeconomy: From sourcing to success stories
Abstract
As the global population continues to grow, so does the demand for longer, healthier lives and environmentally responsible choices. Consumers are increasingly drawn to naturally sourced products with proven health and wellbeing benefits. The marine environment presents a promising yet underexplored resource for the cosmetics industry, offering bioactive compounds with the potential for safe and biocompatible ingredients. This manuscript provides a comprehensive overview of the potential of marine organisms for cosmetics production, highlighting marine-derived compounds and their applications in skin/hair/oral-care products, cosmeceuticals and more. It also lays down critical safety considerations and addresses the methodologies for sourcing marine compounds, including harvesting, the biorefinery concept, use of systems biology for enhanced product development, and the relevant regulatory landscape. The review is enriched by three case studies: design of macroalgal skincare products in Iceland, establishment of a microalgal cosmetics spin-off in Italy, and the utilization of marine proteins for cosmeceutical applications.
... Pigments now play a role not just in aesthetics, but also in enhancing the performance and sustainability of products across many sectors. Due to the rising demand for natural and sustainable beauty products, there has been a shift toward using pigments derived from natural sources [24]. The textile industry is embracing digital pigment printing techniques, allowing for more complex designs and reduced water usage. ...
... Producing pigments from actinomycetes offers promising economic opportunities, especially when considering the growing demand for sustainable alternatives. While synthetic pigments currently benefit from cost-effectiveness due to established petrochemical processes, efficient supply chains, and economies of scale, actinomycete pigment production has distinct advantages [24,[119][120][121]. Although the initial costs associated with fermentation technology and purification methods may be high, these expenses are offset by the increasing consumer preference for eco-friendly products. ...
... For instance, the global natural food colorant market is projected to expand significantly as more consumers opt for natural alternatives to synthetic dyes (https://www.precedenceresearch.com/naturalfood-color-market; accessed on 25 September 2024). Similarly, the cosmetic industry is witnessing a surge in demand for clean beauty products, with consumers increasingly scrutinizing ingredient lists and favoring brands that prioritize sustainability [24]. As such, actinomycete-derived pigments stand to benefit from these market trends, positioning themselves as viable alternatives to both synthetic and traditional natural pigments. ...
Pigment production has a substantial negative impact on the environment, since mining for natural pigments causes ecosystem degradation, while synthetic pigments, derived from petrochemicals, generate toxic by-products that accumulate and persist in aquatic systems due to their resistance to biodegradation. Despite these challenges, pigments remain essential across numerous industries, including the cosmetic, textile, food, automotive, paints and coatings, plastics, and packaging industries. In response to growing consumer demand for sustainable options, there is increasing interest in eco-friendly alternatives, particularly bio-based pigments derived from algae, fungi, and actinomycetes. This shift is largely driven by consumer demand for sustainable options. For bio-pigments, actinomycetes, particularly from the Streptomyces genus, have emerged as a promising green source, aligning with global sustainability goals due to their renewability and biodegradability. Scale-up of production and yield optimization challenges have been circumvented with the aid of biotechnology advancements, including genetic engineering and innovative fermentation and extraction methods, which have enhanced these bio-pigments’ viability and cost-competitiveness. Actinomycete-derived pigments have successfully transitioned from laboratory research to commercialization, showcasing their potential as sustainable and eco-friendly alternatives to synthetic dyes. With the global pigment market valued at approximately USD 24.28 billion in 2023, which is projected to reach USD 36.58 billion by 2030, the economic potential for actinomycete pigments is extensive. This review explores the environmental advantages of actinomycete pigments, their role in modern industry, and the regulatory and commercialization challenges they face, highlighting the importance of these pigments as promising solutions to reduce our reliance on conventional toxic pigments. The successful commercialization of actinomycete pigments can drive an industry-wide transition to environmentally responsible alternatives, offering substantial benefits for human health, safety, and environmental sustainability.
... Cordycepin-loaded hydrogels provide localized delivery to skin lesions, reducing melanoma growth rates by 50% in preclinical models and significantly downregulating pro-inflammatory cytokines such as IL-6 and TNF-α [50]. Polysaccharide-enriched gels accelerate wound healing by 43%, promoting keratinocyte migration and collagen synthesis, while also reducing inflammation markers by 51% [84]. These properties make C. militaris gels effective in managing chronic wounds, inflammatory skin conditions, and post-surgical recovery. ...
Cordyceps militaris is a medicinal mushroom widely utilized in traditional East Asian medicine, recognized for its diverse therapeutic properties. This review explores the potential of C. militaris-derived bioactive gels for applications in dermatology and skincare, with a particular focus on their therapeutic and anti-aging benefits. In response to the rising incidence of skin cancers and the growing demand for natural bioactive ingredients , C. militaris has emerged as a valuable source of functional compounds, including cordycepin, polysaccharides, and adenosine. These compounds exhibit multiple bioac-tivities, including apoptosis induction, cell cycle arrest, and anti-inflammatory effects, which have been shown to be particularly effective against melanoma and other skin cancers. Additionally, the antioxidant properties of C. militaris enhance skin resilience by scavenging reactive oxygen species, reducing oxidative stress, and promoting collagen synthesis, thereby addressing skin health and anti-aging requirements. The potential for incorporating C. militaris compounds into gel-based formulations for skincare is also examined , either as standalone bioactives or in combination with synergistic ingredients. Emphasis is placed on the necessity of clinical trials and standardization to establish the safety, efficacy, and reproducibility of such applications. By providing a safer alternative to synthetic agents, C. militaris-derived bioactive gels represent a promising advancement in dermatology and skincare.
Thraustochytrids, as a distinct group of heterotrophic protists, have garnered considerable attention owing to their remarkable adaptability in extreme marine environments, pronounced capacity for metabolic regulation and prolific production of high-value metabolites. The taxonomic classification of these microorganisms presents a substantial challenge due to the variability in morphological characteristics under different culture conditions. And this undermines the efficacy of traditional classification systems on physiological and biochemical traits. The establishment of a polyphasic taxonomic system integrating genomic characteristics in the future will provide new avenues for more accurate classification and identification. Thraustochytrids can effectively accumulate bioactive substances such as docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), squalene and carotenoids. Through fermentation optimization and genetic modification, scientists have significantly enhanced the production of these metabolites. Moreover, the application of thraustochytrids in aquaculture, poultry and livestock feed has significantly improved animal growth and physiological indicators meanwhile increasing their DHA content. Natural bioactive substances in thraustochytrids, such as terpenoid compounds with antioxidant properties, have been proposed for application in the cosmetics industry. In the field of pharmacology, thraustochytrids have shown certain anti-inflammatory and anti-cancer activities and provide potential for the development of new oral vaccines. Additionally, they can degrade various industrial and agricultural wastes for growth and fatty acid production, demonstrating their potential in environmental bioremediation. Therefore, thraustochytrids not only exhibit tremendous application potential in the field of biotechnology, but also hold significant value in environmental protection and commercialization.
Background
The tunicates form a group of filter-feeding marine animals closely related to vertebrates. They share with them a number of features such as a notochord and a dorsal neural tube in the tadpole larvae of ascidians, one of the three groups that make tunicates. However, a number of typical chordate characters have been lost in different branches of tunicates, a diverse and fast-evolving phylum. Consequently, the tunic, a sort of exoskeleton made of extracellular material including cellulose secreted by the epidermis, is the unifying character defining the tunicate phylum. In the larva of ascidians, the tunic differentiates in the tail into a median fin (with dorsal and ventral extended blades) and a caudal fin.
Results
Here we have performed experiments in the ascidian Phallusia mammillata to address the molecular control of tunic 3D morphogenesis. We have demonstrated that the tail epidermis medio-lateral patterning essential for peripheral nervous system specification also controls tunic elongation into fins. More specifically, when tail epidermis midline identity was abolished by BMP signaling inhibition, or CRISPR/Cas9 inactivation of the transcription factor coding genes Msx or Klf1/2/4/17, median fin did not form. We postulated that this genetic program should regulate effectors of tunic secretion. We thus analyzed the expression and regulation in different ascidian species of two genes acquired by horizontal gene transfer (HGT) from bacteria, CesA coding for a cellulose synthase and Gh6 coding for a cellulase. We have uncovered an unexpected dynamic history of these genes in tunicates and high levels of variability in gene expression and regulation among ascidians. Although, in Phallusia, Gh6 has a regionalized expression in the epidermis compatible with an involvement in fin elongation, our functional studies indicate a minor function during caudal fin formation only.
Conclusions
Our study constitutes an important step in the study of the integration of HGT-acquired genes into developmental networks and a cellulose-based morphogenesis of extracellular material in animals.
Marine collagen sources are potent alternatives due to abundant yield, low pathogen infection risk, high biocompatibility, and any religious and ethical restrictions compared to terrestrial collagen sources. In this research, we aim to investigate the biomaterials potential of the collagen from Aurelia aurita, which is a native jellyfish species in the Marmara Sea. Spectroscopic techniques were used to investigate the structure of jellyfish collagen (JCol) from acid-soluble fraction and compared to Jellagen® from Rhizostoma pulmo. MALDI-TOF showed the main peak of Jellagen® at 276,765.161 Da and jellyfish collagen at 276,761.687 Da. SDS-PAGE indicated α1 and α2 bands at about 122 kDa and 140 kDa, respectively. In FTIR and Raman spectra, the locations of amide bands of both species were almost the same. The pI of JCol was determined as 4.46. The particle size decreased abruptly at 43 oC from 890 to 290 nm. Water, organic and inorganic ratios of collagen were determined at 7.14%, 63.59, and 29.27 respectively. In DSC, the denaturation temperature (Td) of JCol was found at 43.7 oC and found to be higher than that of the collagens from jellyfishes that have been reported so far in the literature. Biocompatibility testing by metabolic assay revealed significantly higher fibroblast proliferation on collagen film than on the Tissue Culture Plate. To conclude, Aurelia aurita collagen would be a suitable source of collagen when biomaterials are needed to have high biocompatibility and unique macromolecular properties such as high denaturation temperatures.
Bioassays are the main tool to decipher bioactivities from natural resources thus their selection and quality are critical for optimal bioprospecting. They are used both in the early stages of compounds isolation/purification/identification, and in later stages to evaluate their safety and efficacy. In this review, we provide a comprehensive overview of the most common bioassays used in the discovery and development of new bioactive compounds with a focus on marine bioresources. We present a comprehensive list of practical considerations for selecting appropriate bioassays and discuss in detail the bioassays typically used to explore antimicrobial, antibiofilm, cytotoxic, antiviral, antioxidant, and anti-ageing potential. The concept of quality control and bioassay validation are introduced, followed by safety considerations, which are critical to advancing bioactive compounds to a higher stage of development. We conclude by providing an application-oriented view focused on the development of pharmaceuticals, food supplements, and cosmetics, the industrial pipelines where currently known marine natural products hold most potential. We highlight the importance of gaining reliable bioassay results, as these serve as a starting point for application-based development and further testing, as well as for consideration by regulatory authorities.
The world’s oceans and seas host >100 known strains of thraustochytrids, a common group of marine eukaryotic unicellular protists, residing in diverse marine habitats, with many others to be isolated and cultivated. The thraustochytrids have become of considerable industrial interest due to health benefits gained from their high percentages of valuable bioactive compounds, revealing the needs for the isolation of new potential strains. Employing a recently developed isolation methodology (use of cell culture medium), we assess initial culture conditions and growth paces of newly isolated thraustochytrid cells (thraustochytrid sp. BSH), originated from the colonial tunicate Botryllus schlosseri, residing on Helgoland Island, Germany. Cells were cultivated under static versus agitated conditions, along with two inoculation sizes (0.5 × 106 and 1 × 106 cells/dish) and in three vessel types (35 mm Petri dishes and T25 and T75 flasks; containing 3, 5 and 15 mL medium, respectively). Cultures were observed under regular microscopy, confocal microscopy and H&E staining. While cells in all conditions grew fast, results revealed the superiority of agitated cultivation in T75 flasks inoculated with 0.5 × 106 cells/dish (6.41 ± 1.91-fold increase/week). Further, 18S rDNA revealed high similarities (99.5–99.8) of strain BSH to two thraustochytrid strains from Monterey, California (USA), B. schlosseri colonies, elucidating a new understanding of these animals-protists associations.
Step towards resilience and sustainability through exploring renewable biomass and waste streams to produce higher-added value products and energy is among key aspects for closing the loops, saving resources, and reducing the resource and emission footprints. In that respective, crustacean shells waste can offer rich spectre of valuable compounds such as proteins, chitin, carotenoids. This waste is produced in large quantities worldwide, thus allowing for commercial valorisation. An overview of technologies is undertaken for more sustainable and environmentally friendly chitosan production via chitin isolation and conversion and compared to the conventional processes. Furthermore, an assessment of the environmental burden and energy demand distribution for conventional and more sustainable alternative processes was performed, based on lab-scale experimental data. Three different chitin extraction routes and three distinct chitosan conversion processes were considered and compared for their greenhouse gas footprint, abiotic depletion, acidification, eutrophication and other potentials. Finally, the energy demand distribution was analysed considering electricity production patterns from three European countries, Slovenia, Portugal and Norway. The results showed that alternatives 3-A and 3-B (conventional eco-solvents - conventional deacetylation with 40 % and 50 % NaOH) generate the lowest environmental burden (184 g CO2 eq./g chitosan). Electricity was the main hotspot of the processes, used either for extraction, plasma treatment or deacetylation. The sensitivity analysis proved that the Norwegian electricity mix has the lowest environmental impact (4.2 g CO2 eq./g chitosan). This study highlights the impact of blue biorefineries by transforming marine waste to valuable biopolymers such as chitin and chitosan.
The growing understanding and knowledge of the potential of marine species, as well as the application of “blue biotechnology” have been motivating new innovative solutions in cosmetics. It is widely noted that that marine species are important sources of compounds with several biological activities that are yet to be discovered. This review explores various biological properties of marine-derived molecules and briefly outlines the main extraction methods. Alongside these, it is well known the legislative and normative framework of cosmetics is increasingly being developed. In this research segment, there is a growing concern with sustainability. In this sense, “blue biotechnology”, together with the use of invasive species or marine waste products to obtain new active ingredients, haven been emerging as innovative and sustainable solutions for the future’s cosmetics industry. This review also examines the regulatory framework and focus on the recent advancements in “blue biotechnology” and its relevance to the sustainable development of innovative cosmetics.
Chitin/chitosan and collagen are two of the most important bioactive compounds, with applications in the pharmaceutical, veterinary, nutraceutical, cosmetic, biomaterials, and other industries. When extracted from non-edible parts of fish and shellfish, by-catches, and invasive species, their use contributes to a more sustainable and circular economy. The present article reviews the scientific knowledge and publication trends along the marine chitin/chitosan and collagen value chains and assesses how researchers, industry players, and end-users can bridge the gap between scientific understanding and industrial applications. Overall, research on chitin/chitosan remains focused on the compound itself rather than its market applications. Still, chitin/chitosan use is expected to increase in food and biomedical applications, while that of collagen is expected to increase in biomedical, cosmetic, pharmaceutical, and nutritional applications. Sustainable practices, such as the reuse of waste materials, contribute to strengthen both value chains; the identified weaknesses include the lack of studies considering market trends, social sustainability, and profitability, as well as insufficient examination of intellectual property rights. Government regulations, market demand, consumer preferences, technological advancements, environmental challenges, and legal frameworks play significant roles in shaping both value chains. Addressing these factors is crucial for seizing opportunities, fostering sustainability, complying with regulations, and maintaining competitiveness in these constantly evolving value chains.
Recent years have seen a lot of interest in mycosporine-like amino acids (MAAs) because of their alleged potential as a natural microbial sunscreen. Since chemical ultraviolet (UV) absorbers are unsafe for long-term usage, the demand for natural UV-absorbing substances has increased. In this situation, MAA is a strong contender for an eco-friendly UV protector. The capacity of MAAs to absorb light in the UV-A (320–400 nm) and UV-B (280–320 nm) range without generating free radicals is potentially relevant in photoprotection. The usage of MAAs for purposes other than photoprotection has now shifted in favor of medicinal applications. Aside from UV absorption, MAAs also have anti-oxidant, anti-inflammatory, wound-healing, anti-photoaging, cell proliferation stimulators, anti-cancer agents, and anti-adipogenic properties. Recently, MAAs application to combat SARS-CoV-2 infection was also investigated. In this review article, we highlight the biomedical applications of MAAs that go beyond photoprotection, which can help in utilizing the MAAs as promising bioactive compounds in both pharmaceutical and cosmetic applications.
Sea cucumbers have high economic value, and in most forms of trade, their body wall is typically the only part that is harvested and sold. The organs of the sea cucumber, collectively known as the viscera, are frequently discarded, contributing to land and water pollution. However, discarded sea cucumber viscera contain various nutrients that can be used in many applications. Therefore, this review highlights the biological and economic aspects of sea cucumbers, followed by a critical discussion of the nutritional value of their internal organs and possible applications, including as functional feed additives in the aquaculture industry, sources of natural testosterone for application in sex reversal and production of monosex population, of neuroprotective agents against central nervous system disorders and of cosmetic ingredients, especially for skin whitening and anti-ageing products. The review further highlights the valorisation potential of viscera to maximize their economic potential, thus providing an enormous prospect for reusing sea cucumber waste, thereby reducing the negative impact of the sea cucumber fishery sector on the environment.
In spite of the significant progress towards sustainable cosmetics, mass-produced sustainable packaging has proven to be a challenge. The complexity of environmental, economic, social, technological, and policy considerations in conjunction with varying consumer behaviors and corporate goals can make it difficult to select an optimal strategy across heterogeneous supply chain components spread over the globe, and the cost and effort of developing, testing, and validating alternative strategies discourages empirical exploration of potential alternatives. This review discusses the challenges that can be expected in the context of broader sustainability efforts, as well as the experience gained in related fields, such as sustainable cosmetics and sustainable packaging, to identify potential pitfalls as well as promising trends towards the development of sustainable color cosmetics packaging. The findings suggest there may be little to be gained from attempting to induce customers to change their behavior, waiting for a significant increase in global recycling infrastructure, or expecting regulatory constraints to substitute for the lack of technological and business solutions. A research strategy is delineated towards the development of sustainable packaging that, with appropriate policy support, could minimize externalities and provide mass-produced packaging that is acceptable to both consumers and producers.
Dissolved organic matter (DOM) occupies a huge and uncharted molecular space. Given its properties, DOM can be presented as a promising biotechnological resource. However, research into bioactivities of DOM is still in early stages. In this study, the biotechnological potential of terrestrial and marine DOM, its molecular composition and their relationships are investigated. Samples were screened for their in vitro antibacterial, antifungal, anticancer and antioxidant activities. Antibacterial activity was detected against Staphylococcus aureus in almost all DOM samples, with freshwater DOM showing the lowest IC 50 values. Most samples also inhibited Staphylococcus epidermidis , and four DOM extracts showed up to fourfold higher potency than the reference drug. Antifungal activity was limited to only porewater DOM towards human dermatophyte Trichophyton rubrum . No significant in vitro anticancer activity was observed. Low antioxidant potential was exerted. The molecular characterization by FT-ICR MS allowed a broad compositional overview. Three main distinguished groups have been identified by PCoA analyses. Antibacterial activities are related to high aromaticity content and highly-unsaturated molecular formulae (O-poor). Antifungal effect is correlated with highly-unsaturated molecular formulae (O-rich). Antioxidant activity is positively related to the presence of double bonds and polyphenols. This study evidenced for the first time antibacterial and antifungal activity in DOM with potential applications in cosmeceutical, pharmaceutical and aquaculture industry. The lack of cytotoxicity and the almost unlimited presence of this organic material may open new avenues in future marine bioprospecting efforts.
Graphical abstract
Marine (blue) biotechnology is an emerging field enabling the valorization of new products and processes with massive potential for innovation and economic growth. In the Mediterranean region, this innovation potential is not exploited as well as in other European regions due to a lack of a clear identification of the different value chains and the high fragmentation of business innovation initiatives. As a result, several opportunities to create an innovative society are being missed. To address this problem, eight Northern Mediterranean countries (Croatia, France, Greece, Italy, Montenegro, Portugal, Slovenia and Spain) established five national blue biotechnology hubs to identify and address the bottlenecks that prevent the development of marine biotechnology in the region. Following a three-step approach (1. Analysis: setting the scene; 2. Transfer: identification of promising value chains; 3. Capitalization: community creation), we identified the three value chains that are most promising for the Northern Mediterranean region: algae production for added-value compounds, integrated multi-trophic aquaculture (IMTA) and valorization aquaculture/fisheries/processing by-products, unavoidable/unwanted catches and discards. The potential for the development and the technical and non-technical skills that are necessary to advance in this exciting field were identified through several stakeholder events which provided valuable insight and feedback that should be addressed for marine biotechnology in the Northern Mediterranean region to reach its full potential.
Environmental concerns regarding the usage of nonrenewable materials are driving up the demand for biodegradable marine biopolymers. Marine biopolymers are gaining increasing attention as sustainable alternatives in various industries, including the food sector. This review article aims to provide a comprehensive overview of marine biopolymers and their applications in the food industry. Marine sources are given attention as innovative resources for the production of sea-originated biopolymers, such as agar, alginate, chitin/chitosan, and carrageenan, which are safe, biodegradable, and are widely employed in a broad spectrum of industrial uses. This article begins by discussing the diverse source materials of marine biopolymers, which encompass biopolymers derived from seaweed and marine animals. It explores the unique characteristics and properties of these biopolymers, highlighting their potential for food applications. Furthermore, this review presents a classification of marine biopolymers, categorizing them based on their chemical composition and structural properties. This classification provides a framework for understanding the versatility and functionality of different marine biopolymers in food systems. This article also delves into the various food applications of marine biopolymers across different sectors, including meat, milk products, fruits, and vegetables. Thus, the motive of this review article is to offer a brief outline of (a) the source materials of marine biopolymers, which incorporates marine biopolymers derived from seaweed and marine animals, (b) a marine biopolymer classification, and (c) the various food applications in different food systems such as meat, milk products, fruits, and vegetables.
Blue Biotechnology is developing rapidly worldwide. However, the Nagoya Protocol (NP), Responsible Research and Innovation (RRI) and other regulatory requirements in this field are falling behind. This article identifies the main RRI, NP, and regulatory gaps and provides key recommendations to mitigate these challenges.
Introduction: Marine-derived compounds, such as seaweed extracts, fucoidan and ulvans, and ectoin, have gained attention in recent years due to their unique structural and functional characteristics, which make them attractive ingredients for skincare products. In this study, we developed a serum spray based on fucoidan, Ulva lactuca extract, and ectoin and evaluated its efficacy on facial skin. Materials and Methods: A split-face design dermatological evaluation of the serum spray was conducted on 33 subjects with visible signs of skin aging, with 29 subjects completing the study according to its protocol. The subjects had a mean age of 50 years and 16 had sensitive skin. The instrumental efficacy and subjective efficacy of the spray were measured on facial skin by evaluating the trans-epidermal water loss (TEWL), skin pH, skin roughness/wrinkle reduction, and skin hydration at baseline, 20 min after its application and 28 days after its continuous use. Results: We found that the application of the serum spray did not significantly affect the TEWL. The hydration in the area treated with Moisturizer + Spray was 17% higher than that in the area treated with Moisturizer alone after 20 min of application (p < 0.001) and 5% higher after 28 days of use (p < 0.05). Twenty minutes after the application of the product, the average roughness in the area treated with Moisturizer + Spray decreased significantly, with an average of 7% compared to baseline (p < 0.001). With regard to the long-term antiwrinkle effect, 28 days after the continuous use of the product, the average roughness in the area treated with Moisturizer + Spray decreased significantly, with an average of 17% in relation to baseline (p < 0.001). The skin pH was significantly lowered by 6% after 28 days of use of the moisturizer + spray (p < 0.05). Conclusion: The results of this study suggest that the marine-derived compounds fucoidan, ulvans, and ectoin have hydrating and anti-wrinkle properties that make them effective ingredients for skincare products. The serum spray developed in this study was demonstrated to be safe and increase hydration, showing a reduction in wrinkles and maintenance of the skin barrier function after 28 days of its continuous use. Therefore, it could be a promising addition to skincare products for improving skin health.
Introduction
The up-side down jellyfish Cassiopea andromeda represents a yet untapped marine species that could be targeted as a new source for functional ingredients, such as natural pigments and antioxidants. Since C. andromeda hosts endosymbiotic dinoflagellates, this jellyfish contains peridinin pigments, which are linked with high antioxidant capacities and many other health-promoting properties. This study investigates the potential to specifically increase the content of peridinin and overall antioxidant activity in C. andromeda, through the targeted application of different photosynthetic active radiation (PAR) intensities and ultraviolet radiation, cultured in fully controlled indoor aquaculture systems.
Materials and Methods
Indoor bred C. andromeda specimens were exposed to five different PAR intensities (50, 100, 200, 400 and 800 µmol photons m⁻² s⁻¹) and a combined treatment of narrow-band UVB (λ = 285 ± 10 nm) radiation and intermediate (200 µmol photons m⁻² s⁻¹) PAR intensity over a period of four weeks. Before the treatment and after two- and four-week treatment intervals, pigment concentrations and antioxidant activity levels were measured using high-performance liquid chromatography (HPLC) and the Trolox Equivalent AntioxidantCapacity (TEAC) assay, respectively. In addition, relative growth rate, umbrella pulsation and photosynthetic efficiency (measured by pulse amplitude modulated fluorometry) of C. andromeda individuals were also monitored throughout the experiment.
Results
Chlorophyll a (Chl a) and peridinin (Per) dominated overall pigment content in C. andromeda endosymbionts, chlorophyll c2 and diadinoxanthin were detected in minor amounts. Over the treatment time, Chl a and Per concentrations, measured as µg g⁻¹ jellyfish dry weight and pg microalgae-cell⁻¹, decreased sharply at higher PAR intensities (200 – 800 µmol photons m⁻² s⁻¹) compared to the control treatment (100 µmol photons m⁻² s⁻¹). After four weeks Chl a and Per concentrations were lowest at the highest PAR intensity (800 µmol photons m⁻² s⁻¹) and highest at the lowest PAR intensity (50 µmol photons m⁻² s⁻¹). Moreover, the ratio of Chl a and Per showed a relative decrease of Per with increasing PAR intensity. The combined treatment of narrow-band UVB (λ = 285 ± 10 nm) radiation and intermediate (200 µmol photons m⁻² s⁻¹) PAR intensity led to significantly elevated Chl a and Per concentrations compared to the 200 µmol photons m⁻² s⁻¹ PAR treatment without UVB. Significantly elevated antioxidant activity levels, measured as Trolox Equivalents mmol g⁻¹ jellyfish dry weight, were only detected in UVB exposed C. andromeda, indicating that Chl a and Per did not determine overall antioxidant capacity. The photosynthetic efficiency of C. andromeda endosymbionts was not affected by elevated antioxidant activity. Opposing that, the jellyfish hosts that were exposed to the UVB treatment shrunk drastically, indicating a strong stress response.
Discussion
With this study, we demonstrate for the first time the application potential of PAR intensity manipulations and UVB irradiation, to increase the content of valuable pigments and antioxidants in C. andromeda jellyfish and their endosymbiotic dinoflagellates that live in hospite within the host tissue. Based on these findings, we propose the culture of C. andromeda under fully controlled and light-optimized conditions as new pathway to harness bioproducts and functional ingredients.
Seaweeds or algae are marine autotrophic organisms. They produce nutrients (e.g., proteins, carbohydrates, etc.) essential for the survival of living organisms as they participate in biochemical processes and non-nutritive molecules (such as dietary fibers and secondary metabolites), which can improve their physiological functions. Seaweed polysaccharides, fatty acids, peptides, ter-penoids, pigments, and polyphenols have biological properties that can be used to develop food supplements and nutricosmetic products as they can act as antibacterial, antiviral, antioxidant, and anti-inflammatory compounds. This review examines the (primary and secondary) metabolites produced by algae, the most recent evidence of their effect on human health conditions, with particular attention to what concerns the skin and hair's well-being. It also evaluates the industrial potential of recovering these metabolites from biomass produced by algae used to clean wastewater. The results demonstrate that algae can be considered a natural source of bioactive molecules for well-being formulations. The primary and secondary metabolites' upcycling can be an exciting opportunity to safeguard the planet (promoting a circular economy) and, at the same time, obtain low-cost bioactive molecules for the food, cosmetic, and pharmaceutical industries from low-cost, raw, and renewable materials. Today's lack of methodologies for recovering bioactive molecules in large-scale processes limits practical realization.
Objective
There are many reasons why marine resources are currently being examined extensively. One of the causes is that 70% of the earth's surface is covered by oceans. From marine resources, some natural products and their derivatives are derived. These marine natural products are produced by the following organisms: Marine invertebrates, marine algae, and marine microbes are the three main biological classes. An overview of the marine natural products (MNPs) industry is presented, along with a current list of medications that the FDA has approved as well as those that are undergoing clinical trials and could be regarded as MNP derivatives.
Methods
Secondary metabolites from marine organisms such as snails, tunicates, sponges, soft corals, and mollusks have been extracted to produce bio active compounds, which have sparked the creation of new classes of therapeutic agents. Marine life is a significant source of structurally diverse and biologically active secondary metabolites.
Key findings
It is observed that marine source is one of the powerful way of producing products that are natural and effective.
Conclusion
Some commercially available preparations treat diseases like HIV, cancer, and various skin conditions using their collected resources
Marine bioprospecting is an activity that has only been developed recently. The term refers to the exploration and commercial exploitation of marine genetic resources. It is a promising but also highly controversial activity, which is expected to experience significant growth in the next decades, offering vast economic and commercial profits. At the same time, it raises several environmental, scientific, ethical, and legal challenges that will need to be addressed. In order to increase understanding about marine bioprospecting and its overall impact, this paper aims to shed more light on the activity and briefly present some of the resulting challenges.
Carotenoids and squalene are important terpenes that are applied in a wide range of products in foods and cosmetics. Thraustochytrids might be used as alternative production organisms to improve production processes, but the taxon is rarely studied. A screening of 62 strains of thraustochytrids sensu lato for their potential to produce carotenoids and squalene was performed. A phylogenetic tree was built based on 18S rRNA gene sequences for taxonomic classification, revealing eight different clades of thraustochytrids. Design of experiments (DoE) and growth models identified high amounts of glucose (up to 60 g/L) and yeast extract (up to 15 g/L) as important factors for most of the strains. Squalene and carotenoid production was studied by UHPLC-PDA-MS measurements. Cluster analysis of the carotenoid composition partially mirrored the phylogenetic results, indicating a possible use for chemotaxonomy. Strains in five clades produced carotenoids. Squalene was found in all analyzed strains. Carotenoid and squalene synthesis was dependent on the strain, medium composition and solidity. Strains related to Thraustochytrium aureum and Thraustochytriidae sp. are promising candidates for carotenoid synthesis. Strains closely related to Schizochytrium aggregatum might be suitable for squalene production. Thraustochytrium striatum might be a good compromise for the production of both molecule groups.
The manufacturing and consumption of plastic products have steadily increased over the past decades due to rising global demand, resulting not only in the depletion of petroleum resources but also increased environmental pollution due to the non-biodegradable nature of conventional plastics. Moreover, despite being introduced into the market as an alternative to conventional petroleum-based plastics, biobased plastics are mainly manufactured from agricultural crop-based sources, which has negative impacts on the environment and the livelihoods of people. Marine-derived bioplastics are becoming a promising and cost-effective solution to the rising demand for plastic products. The physicochemical, biological, and degradation properties of marine-derived bioplastics have made them promising substances for many applications. However, more research is required for their large-scale implementation. Therefore, this review summarizes the raw materials of marine-derived bioplastics such as algae, animals, and microorganisms, as well as their extraction processes and properties. These insights could thus accelerate the production of marine-derived bioplastics as a novel alternative to prevailing bioplastics by taking advantage of marine biomass.
Aging is an irreversible physiological process in the human body, and the aging characteristics of the body that accompany this process also lead to many other chronic diseases, such as neurodegenerative diseases represented by Alzheimer’s disease and Parkinson’s disease, cardiovascular diseases, hypertension, obesity, cancer, and so on. The marine environment is highly biodiverse, the natural active products of these organisms constitute a vast treasure trove of marine drugs or drug candidates that play an essential role in disease prevention and treatment, and the active peptide products among them have received special attention because of their unique chemical properties. Therefore, the development of marine peptide compounds as anti-aging drugs is emerging as an important research area. This review highlights the currently available data on marine bioactive peptides with anti-aging potential from 2000 to 2022 by analyzing the prevalent aging mechanisms, critical aging metabolic pathways and well-established multi-omics aging characteristics, as well as grouping different bioactive and biological species lines of peptides from marine organisms and discussing their research modalities and functional characteristics. Active marine peptides is a promising topic to explore and to develop their potential as anti-aging drugs or drug candidates. We expect this review to be instructive for future marine drug development and to reveal new directions for future biopharmaceuticals.
Indole has an increasing interest in the flavor and fragrance industry. It is used in dairy products, tea drinks, and fine fragrances due to its distinct floral odor typical of jasmine blossoms. The current production of indole based on isolation from coal tar is non-sustainable and its isolation from plants is often unprofitable due to low yields. To offer an alternative to the conventional production, biosynthesis of indole has been studied recently. A glucose-based indole production was achieved by employing the Corynebacterium glutamicum tryptophan synthase α-subunit (TrpA) or indole-3-glycerol phosphate lyase (IGL) from wheat Triticum aestivum in a genetically-engineered C. glutamicum strain. In addition, a highly efficient bioconversion process using C. glutamicum heterologously expressing tryptophanase gene (tnaA) from Providencia rettgeri as a biocatalyst was developed. In this work, de novo indole production from glucose was enabled by expressing the P. rettgeri tnaA in a tryptophan-producing C. glutamicum strain. By metabolic engineering of a C. glutamicum shikimate accumulating base strain, tryptophan production of 2.14 ± 0.02 g L⁻¹ was achieved. Introduction of the tryptophanase form P. rettgeri enabled indole production, but to low titers, which could be improved by sequestering indole into the water-immiscible solvent tributyrin during fermentation and a titer of 1.38 ± 0.04 g L⁻¹ was achieved. The process was accelerated by decoupling growth from production increasing the volumetric productivity about 4-fold to 0.08 g L⁻¹ h⁻¹.
Key points
• Efficient de novo indole production via tryptophanases from glucose
• Increased indole titers by product sequestration and improved precursor supply
• Decoupling growth from production accelerated indole production
Brown seaweeds, due to their wide range of bioactive compounds, have a high ability to inhibit free radicals and protect against ultraviolet rays. In the present study, the ethyl acetate fraction (EF) was isolated from the Padina boergesenii brown seaweed. Antioxidant activity (by the DPPH scavenging activity method) and cytotoxicity against UVB-induced cytotoxicity in HaCaT human keratinocytes were evaluated. Then, this fraction was used as a bio-filter in the formulation of sun-screen, and the physical properties and stability were investigated. The results showed that the EF could inhibit DPPH radical scavenging (54 ± 1%) and cell viability of HaCaT keratinocytes exposed to UVB irradiation (81.2 ± 0.1%). The results of the stability study of the cream formulated with EF showed that at temperatures 4 °C and 25 °C it has high stability; and at 40 °C on the 28th day, a slight decrease in its stability was observed. The pH and Sun Protection Factor of the cream formulated with EF were reported at 5.8 and 20.55, respectively. Also, the DPPH scavenging activity of the cream was not altered for 28 days of storage at temperatures of 4-40 °C. According to our results, it was proved that the sunscreen formulated with EF of P. boergesenii brown seaweed has promising properties and characteristics that can create a new opportunity for the development of cosmetics and skin care products.
Pigments have been used since historical times and are currently used in food, cosmetic, pharmaceutical, and other industries. One of the main sources of natural pigments are plants and insects; however, microorganisms are of great interest due to their bioactivities and advantages in their production. Actinobacteria, especially the genus Streptomyces, are biotechnologically valuable, producing specialized metabolites with a broad spectrum of bioactivities, such as antioxidant, anticancer, antibiofilm, antifouling, and antibiotic activities, as well as pigments, among others. In this review, we identify, summarize, and evaluate the evidence regarding the potential of Streptomyces strains to be biological sources of bioactive pigments. To conclude, future research will include purifying pigmented extracts that have already been reported, studying the purified compounds in a specific application, isolating new microorganisms from new isolation sources, improving the production of pigments already identified, modifying culture media or using new technologies, and developing new extraction techniques and a wide range of solvents that are ecofriendly and efficient.
The global cosmetics market reached US800 billion by 2023, at around a 7% annual growth rate. The cosmetics industry is emerging as one of the fastest-growing industries of the past decade. Data shows that the Chinese cosmetics market was US450 billion. The influence of social media and the internet has raised awareness of the risks associated with the usage of many chemicals in cosmetics and the health benefits of natural products derived from plants and other natural resources. As a result, the cosmetic industry is now paying more attention to natural products. The present review focus on the possible applications of natural products from various biological sources in skin care cosmetics, including topical care products, fragrances, moisturizers, UV protective, and anti-wrinkle products. In addition, the mechanisms of targets for evaluation of active ingredients in cosmetics and the possible benefits of these bioactive compounds in rejuvenation and health, and their potential role in cosmetics are also discussed.
The market demand for marine-based cosmetics has shown a tremendous growth rate in the last decade. Marine resources represent a promising source of novel bioactive compounds for new cosmetic ingredient development. However, concern about sustainability also becomes an issue that should be considered in developing cosmetic ingredients. The fisheries industry (e.g., fishing, farming, and processing) generates large amounts of leftovers containing valuable substances, which are potent sources of cosmeceutical ingredients. Several bioactive substances could be extracted from the marine by-product that can be utilized as a potent ingredient to develop cosmetics products. Those bioactive substances (e.g., collagen from fish waste and chitin from crustacean waste) could be utilized as anti-photoaging, anti-wrinkle, skin barrier, and hair care products. From this perspective, this review aims to approach the potential active ingredients derived from marine by-products for cosmetics and discuss the possible activity of those active ingredients in promoting human beauty. In addition, this review also covers the prospect and challenge of using marine by-products toward the emerging concept of sustainable blue cosmetics.
Plant biofactories are a promising platform for sustainable production of high-value compounds, among which are insect sex pheromones, a green alternative to conventional insecticides in agriculture. Recently, we have constructed transgenic Nicotiana benthamiana plants (“Sexy Plants”, SxP) that successfully produce a blend of moth (Lepidoptera) sex pheromone compounds (Z)-11-hexadecen-1-ol and (Z)-11-hexadecenyl acetate. However, efficient biosynthesis of sex pheromones resulted in growth and developmental penalty, diminishing the potential for commercial use of SxP in biomanufacturing. To gain insight into the underlying molecular responses, we analysed the whole-genome transcriptome and evaluated it in relation to growth and pheromone production in low- and high-producing transgenic plants of v1.0 and v1.2 SxP lines. In our study, high-producing SxPv1.2 plants accumulated the highest amounts of pheromones but still maintained better growth compared to v1.0 high producers. For an in-depth biological interpretation of the transcriptomic data, we have prepared a comprehensive functional N. benthamiana genome annotation as well as gene translations to Arabidopsis thaliana, enabling functional information transfer by using Arabidopsis knowledge networks. Differential gene expression analysis, contrasting pheromone producers to wild-type plants, revealed that while only a few genes were differentially regulated in low-producing plants, high-producing plants exhibited vast transcriptional reprogramming. They showed signs of stress-like response, manifested as downregulation of photosynthesis-related genes and significant differences in expression of hormonal signalling and secondary metabolism-related genes, the latter presumably leading to previously reported volatilome changes. Further network analyses confirmed stress-like response with activation of jasmonic acid and downregulation of gibberellic acid signalling, illuminating the possibility that the observed growth penalty was not solely a consequence of a higher metabolic burden imposed upon constitutive expression of a heterologous biosynthetic pathway, but rather the result of signalling pathway perturbation. Our work presents an example of comprehensive transcriptomic analyses of disadvantageous stress signalling in N. benthamiana biofactory that could be applied to other bioproduction systems.
Fucoxanthin is one of the light-harvesting pigments in brown microalgae, which is increasingly gaining attention due to its numerous health-promoting properties. Currently, the production of microalgal fucoxanthin is not yet feasible from an economic perspective. However, the cultivation of microalgae at favourable conditions holds great potential to increase the viability of this fucoxanthin source. Hence, this study aimed to review the fucoxanthin production of microalgae under different conditions systematically. A literature search was performed using the Web of Science, Scopus and PubMed databases. A total of 188 articles were downloaded and 28 articles were selected for the current review by two independent authors. Microalgae appeared to be a more reliable fucoxanthin source compared to macroalgae. Overall, a consensus fucoxanthin production condition was obtained and proposed: light intensity ranging from 10 to 100 µmol/m2/s could achieve a higher fucoxanthin content. However, the optimal light condition in producing fucoxanthin is species-specific. The current review serves as an antecedent by offering insights into the fucoxanthin-producing microalgae response to different culture factors via a systematic analysis. With the current findings and recommendations, the feasibility of producing fucoxanthin commercially could be enhanced and possibly achieve practical and sustainable fucoxanthin production.
BACKGROUND
Owing to their biodiversity, many valuable natural compounds have been extracted from marine resources and used in the development of pharmaceutical, nutraceutical and cosmeceutical products. The New Zealand black‐footed abalone ( Haliotis iris ) is known to host a variety of bioactive compounds in its flesh.
RESULTS
Water‐soluble bioactive compounds were extracted from H. iris by subcritical water extraction technique, and the effect of subcritical water temperature (110–280 °C) on the extraction performance was studied. The highest antioxidant activity and subsequently glycogen and phenolic content, as well as concentration of bioactives, were found at temperatures between 220 and 250 °C. The carbohydrate content of the extracts peaked at 110 °C, which was then degraded at higher temperatures. Protein and amino acid contents of H. iris extracts were also decreased as the temperature increased above 160 °C. Furthermore, the H. iris extracts were found to be non‐toxic.
CONCLUSION
The results indicate that extraction temperature has a significant impact on the bioactivity of H. iris extracts. Subcritical water extraction can be used in place of more traditional techniques to create high‐quality abalone extracts. © 2022 The Authors. Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).
Solid wastes from domestic, industrial and agricultural sectors cause acute economic and environmental problems. These issues can be partly solved by anaerobic digestion of wastes, yet this process is incomplete and generates abundant byproducts as digestate. Therefore, cultivating mixotrophic algae on anaerobic digestate appears as a promising solution for nutrient recovery, pollutant removal and biofuel production. Here we review mixotrophic algal cultivation on anaerobic waste digestate with focus on digestate types and characterization, issues of recycling digestate in agriculture, removal of contaminants, and production of biofuels such as biogas, bioethanol, biodiesel and dihydrogen. We also discuss applications in cosmetics and economical aspects. Mixotrophic algal cultivation completely removes ammonium, phosphorus, 17β-estradiol from diluted digestate, and removes 62% of zinc, 84% of manganese, 74% of cadmium and 99% of copper.
Background:
Guided by ethical considerations and regulatory requirements such as the 7th Amendment to the European Cosmetics Directive N° 1223/2009, the cosmetic industry has developed and evaluated alternative test strategies such as in vitro assays, in silico approaches for toxicological endpoints and efficacy of cosmetic products and cosmetics ingredients. In consequence, the European Centre for the Validation of Alternative Methods (ECVAM) has proposed a list of validated cell-based in vitro models for predicting the safety and toxicity of cosmetic ingredients. These models have been demonstrated as valuable and effective tools to overcome the limitations of animal in vivo studies. For example, 3D human skin equivalent models are used to evaluate skin irritation potential; and excised human skin is used as the gold standard for the evaluation of dermal absorption.
Objective:
This review presents, in relation to the regulatory requirements, the main alternative in vitro models used in the safety tests of cosmetic products, focusing on skin sensitization, skin corrosion, skin irritation and skin absorption, with advantages and limitations of each model. Recent innovative 3D cell technologies such as Organ-on-a-Chip (OoC) models that can bring significant improvements for toxicology and efficacy testing are also presented.
Conclusion:
The development of OoC technology is promising for assessing the toxicity of substances contained in cosmetics, particularly for repeated dose toxicity, for which no alternative in vitro methods are currently available. Nevertheless, aside from the challenges, the technology needs to be validated and accepted by regulatory organizations as an effective method. Collaboration between researchers, regulatory organizations and industry would be required to achieve this validation.
Phycobiliproteins (PBPs) are colored and water-soluble biliproteins found in cyanobacteria, rhodophytes, cryptomonads and cyanelles. They are divided into three main types: allophycocyanin, phycocyanin and phycoerythrin, according to their spectral properties. There are two methods for PBPs preparation. One is the extraction and purification of native PBPs from Cyanobacteria, Cryptophyta and Rhodophyta, and the other way is the production of recombinant PBPs by heterologous hosts. Apart from their function as light-harvesting antenna in photosynthesis, PBPs can be used as food colorants, nutraceuticals and fluorescent probes in immunofluorescence analysis. An increasing number of reports have revealed their pharmaceutical potentials such as antioxidant, anti-tumor, anti-inflammatory and antidiabetic effects. The advances in PBP biogenesis make it feasible to construct novel PBPs with various activities and produce recombinant PBPs by heterologous hosts at low cost. In this review, we present a critical overview on the productions, characterization and pharmaceutical potentials of PBPs, and discuss the key issues and future perspectives on the exploration of these valuable proteins.
As the market size of the cosmetics industry increases, the safety and effectiveness of new products face higher requirements. The marine environment selects for species of micro-organisms with metabolic pathways and adaptation mechanisms different from those of terrestrial organisms, resulting in their natural products exhibiting unique structures, high diversity, and significant biological activities. Natural products are usually safe and non-polluting. Therefore, considerable effort has been devoted to searching for cosmetic ingredients that are effective, safe, and natural for marine micro-organisms. However, marine micro-organisms can be difficult, or impossible, to culture because of their special environmental requirements. Metagenomics technology can help to solve this problem. Moreover, using marine species to produce more green and environmentally friendly products through biotransformation has become a new choice for cosmetic manufacturers. In this study, the natural products of marine micro-organisms are reviewed and evaluated with respect to various cosmetic applications.
The development of alternative methods to animal testing has gained great momentum since Russel and Burch introduced the "3Rs" concept of Reduction, Refinement, and Replacement of animals in safety testing in 1959. Several alternatives to animal testing have since been introduced, including but not limited to in vitro and in chemico test systems, in silico models, and computational models (e.g., [quantitative] structural activity relationship models, high-throughput screens, organ-on-chip models, and genomics or bioinformatics) to predict chemical toxicity. Furthermore, several agencies have developed robust integrated testing strategies to determine chemical toxicity. The cosmetics sector is pioneering the adoption of alternative methodologies for safety evaluations, and other sectors are aiming to completely abandon animal testing by 2035. However, beyond the use of in vitro genetic testing, agencies regulating the food industry have been slow to implement alternative methodologies into safety evaluations compared with other sectors; setting health-based guidance values for food ingredients requires data from systemic toxicity, and to date, no standalone validated alternative models to assess systemic toxicity exist. The abovementioned models show promise for assessing systemic toxicity with further research. In this paper, we review the current alternatives and their applicability and limitations in food safety evaluations.
Jellyfish have been deemed as a viable food source for centuries, however little is known regarding its collagen and its bioactive qualities. With the rise of demand in marine resources, the potential of jellyfish collagen to be applied in the pharmaceutical, medicinal, cosmeceutical and nutraceutical sectors cannot be ignored. In this chapter, we collate the prevalent methods to extract the jellyfish collagen, including acid-solubilized and pepsin-soluble-solubilized method, and physical assisted acid-extraction method, each yielding various percentage of jellyfish collagen extract. Aside from that, this chapter also discusses the applications of jellyfish collagen in numerous industries, thanks to studies investigating their benefits to mankind when consumed. More studies should be done to further characterize jellyfish collagen from different species; however, preventive measures should be taken note of so that exploitation of jellyfish does not occur.
The skin surface lipid (SSL) is composed of lipids originated from sebum and epidermis. Sebum is produced entirely by the sebaceous glands. Many conditions can affect the function of sebaceous glands. This function can be evaluated through analyzing the composition of sebum, measuring the quantity of sebum excretion, and evaluating glandular parameters. Many methods have been developed to determine SSL and sebum function objectively which are based on solvent extraction, bentonite gel, absorbent paper, frosted glass, and plastic film collection.
The separation of microalgae biomass from diluted cultures found in large-scale production systems is a major challenge for microalgae-related processes. Conventional methods use centrifugation but it requires too much energy (1 kWh/m³), the combination with previous pre-concentration operations such as settling or flotation is needed to reduce this energy consumption. However, both settling and flotation of microalgae biomass require the addition of chemical coagulants/flocculants, thus contaminating the biomass. This work explores the potential use of Electro-Coagulation-Flocculation (ECF) as a method for the pre-concentration of microalgae biomass. Thus, the recovery of freshwater microalgae Scenedesmus almeriensis was studied, both in batch and continuous mode, using this technique to optimize the operating conditions. Data from batch experiments show as a minimum conductivity of 5 mS∙cm⁻¹ is required, the electrical potential of 12 V and current density of 18 mA∙cm⁻² allowing for the recovery of 90% of the biomass, while the biomass recovery increases up to 99% when increasing the current density to 23 mA∙cm⁻². The efficiency of the process increases when increasing the biomass concentration, thus energy consumption reduces by half from 1.0 to 0.5 kWh∙Kg⁻¹ when the biomass concentration increases from 2.8 to 5.0 g∙L⁻¹. To optimize the performance of the system experiments were performed in continuous mode maintaining the voltage and current density at 12 V and 47 mA∙cm⁻² respectively, but modifying the flow rate and biomass concentration provided to the electrocoagulation chamber. Data shows as the optimal residence time is 15 s whereas the energy consumption reduces from 3.2 to 0.58 kWh∙Kg⁻¹ when increasing the biomass concentration from 0.75 to 4.20 g∙L⁻¹. Results confirm the reliability of this method for the pre-concentration of microalgae biomass, it being a fast process consuming reasonable energy. Further optimization of the electrocoagulation chamber and electrical conditions will improve the efficiency of this system for further commercial processes while keeping its major advantages of non-contaminating the biomass and allowing to achieve high biomass recovery values in short times.
Carbohydrates and their implications for human health have been the subject to a rapidly growing interest. Substantial advances in analytical methods have enabled a more effective assessment of carbohydrates and their pharmacological effects. Developing a carbohydrate profile technology would surely aid the understanding of carbohydrate dietary impacts. With the advances in technology for characterization, as well as exploration of complex structure, it is becoming more feasible to synthesize such compounds, rather than isolation. Several technological developments, including improved analytical tools, glycomics, and automation technology, have opened up new opportunities to globally assess most carbohydrates in envisaged samples. The main analytical methods applied to carbohydrates are described. And then the development of automation technology in glycan synthesis are introduced. This review concludes by considering the limitations of the existing technologies and required future developments for overcoming these limitations and improving identification score and/or yield.
Cosmetic industries have expanded globally and will continue to increase as there are consumers. Nowadays, the interest starts to incline towards cosmetics formulated using plant-based ingredients. Marine plants such as seaweed possess numerous natural polysaccharides. Carrageenan is one of the compounds that can be extracted from red seaweed (Rhodophyta). This natural polysaccharide is widely known to act as a thickener, stabilizer, and water-binding agent as well as have diverse biological activities that make it a suitable active ingredient in cosmetic products. The review paper is organized by starting with discussing the significant aspects related to carrageenan which are the source, structure, as well as general and biological properties of carrageenan that make it appropriate to be applied in cosmetics. This paper also highlighted the applications of carrageenan in cosmetics, followed by the extraction method and instrument used.
In the last few decades, attention to new natural antimicrobial compounds has arisen due to a change in consumer preferences and the increase in the number of resistant microorganisms. Algae are defined as photosynthetic organisms that demonstrate a wide range of adaptability to adverse environmental conditions like temperature extremes, photo-oxidation, high or low salinity, and osmotic stress. Algae are primarily known to produce large amounts of secondary metabolite against various kinds of pathogenic microbes. Among these algae, micro and microalgae of river, lake, and algae of oceanic origin have been reported to have antimicrobial activity against the bacteria and fungi of pathogenic nature. Various polar and non- polar extracts of micro- and macroalgae have been used for the suppression of these pathogenic fungi. Apart from these, certain algal derivatives have also been isolated from these having antibacterial and antifungal potential. Among the bioactive molecules of algae, polysaccharides, sulphated polysaccharides, phycocyanobilin polyphenols, lectins, proteins lutein, vitamin E, B12 and K1, peptides, polyunsaturated fatty acids and pigments can be highlighted. In the present review, we will discuss the biological activity of these derived compounds as antifungal/ antibacterial agents and their most promising applications. A brief outline is also given for the prospects of these isolated phytochemicals and using algae as therapeutic in the dietary form. We have also tried to answer whether alga-derived metabolites can serve as potential therapeutics for the treatment of SARS-CoV-2-like viral infections too.
Polysaccharides have attracted much attention due to their significant bio-activities. This review aims to summarize the main polysaccharides sources of related polysaccharides from algae, plants, fungus, and bacteria, and give insights into the structure-activity relationship between the moisture retention and structural characteristics of polysaccharides. The molecular weight, functional groups, polysaccharide modifications and apparent structure of polysaccharides are closely related to the moisturizing properties of polysaccharides in terms of moisturizing conformation. Based on recent moisturizing pieces of evidence, we propose a new framework focusing on the moisturizing intrinsic and extrinsic mechanisms. Polysaccharides molecular weight has different effects on moisturizing property. The extrinsic moisturization is mainly via the formation of hydrogen bonds between polysaccharides, the intrinsic moisturizing is mainly by regulating the production of some tight junction proteins. Accordingly, this review could further open the door for the production and application of polysaccharides as novel moisturizing agents in the cosmetic field.
Squalene, [oxidized form squalane] is a terpenoid with biological activity that produced by animals and plants. In the human body, a significant excretion named as sebum includes squalene in 12 percent. This bioactive compound shows anti-inflammatory, detoxifying, moisturizing and antioxidant effects on the human body. In addition to having these properties, it is known that squalene production decreases as less sebum is produced with age. Because of that, the need for supplementation of squalene through products has arisen. As a result, squalene production has been drawn attention due to its many application possibilities by cosmetic, cosmeceutical and pharmaceutical fields. At this point, approximately 3,000 of sharks, the major and the most popular source of squalene must be killed to obtain 1 ton of squalene. These animals are on the verge of extinction. This situation has caused to focus on finding microalgae strains, which are sustainable producers of squalene as alternative to sharks. This review paper summarizes the recent progresses in the topic of squalene. For this purpose, it contains information on squalene producers, microalgal squalene production and cosmetic evaluation of squalene.