An organizational culture is a mindset, expressed through and influenced by tangible and intangible means, which characterizes an institution and its operation. More simply, it could be described as a philosophy of operation embraced by an organization and expressed through both visual and nonvisual means. Organizational cultures exist in all establishments, and they have a significant bearing on institutional performance. An organization’s culture, if well devised and properly managed, affords health and medical institutions with a strategic asset and key competitive advantage. Importantly, organizational culture educates and enlightens personnel regarding perspectives and practices deemed to be appropriate and acceptable, providing direction as duties and responsibilities are being completed. This is especially important in health and medical settings, as direct oversight often is not possible. Not unlike guidance systems used by rockets journeying into space, ensuring that they stay on course, a productive organizational culture helps healthcare establishments stay on course by creating an institutional mindset that guides employees and their associated decisions and actions. This chapter dedicates attention toward understanding organizational culture in healthcare institutions, complete with guidance on how to foster the development of cultures that are productive.