The organizational structure of an establishment refers to an institution’s hierarchy, depicting its chain of command, arrangement of departments, and associated reporting relationships within the organization. Organizational structure activities, including organizational chart assembly, represent the most notable pursuits of organization design, a component of organizational behavior and management which focuses on the process of formulating, implementing, and evaluating institutional structures, activities, and operations in a manner which facilitates the fulfillment of an organization’s mission. When healthcare organizations are established, a full range of decisions and actions concerning institutional structures and work processes are required. As they mature, these operational facets must evolve to accommodate any changes encountered. While options abound for structuring and designing organizations in manners desired, as with anything, prudent decisions must be made in order to realize success. The better designed a healthcare establishment, the better able it will be to perform. By deploying the knowledge and skills conveyed in this chapter, healthcare providers will be able to aid their given institutions in harnessing the power of organizational structure and design, affording opportunities to execute operations successfully, ultimately leading to mission fulfillment.