Follicular fluid high density lipoprotein-associated micronutrient levels are associated with embryo fragmentation during IVF

Department of Biotechnical and Clinical Laboratory Sciences, University at Buffalo, State University of New York, 26 Cary Hall, 3435 Main Street, Buffalo, NY 14214, USA.
Journal of Assisted Reproduction and Genetics (Impact Factor: 1.72). 11/2009; 26(11-12):557-60. DOI: 10.1007/s10815-009-9367-x
Source: PubMed


To investigate whether follicular fluid lipid-soluble micronutrients are associated with embryo morphology parameters during IVF.
Follicle fluid and oocytes were obtained prospectively from 81 women. Embryo morphology parameters were used as surrogate markers of oocyte health. HDL lipids and lipid-soluble micronutrients were analyzed by high-pressure liquid chromatography. Non-parametric bi-variate analysis and multivariable ordinal logistic regression models were employed to examine associations between biochemical and embryo morphology parameters.
Follicular fluid HDL cholesterol (r = -0.47, p < 0.01), alpha-tocopherol (r = -0.41, p < 0.01), delta-tocopherol (r = -0.38, p < 0.05) and beta-cryptoxanthine (r = -0.42, p < 0.01) are negatively correlated with embryo fragmentation. Ordinal logistic regression models indicate that a 0.1 mumol/L increase in beta-cryptoxanthine, adjusted for gamma-tocopherol, is associated with a 75% decrease in the cumulative odds of higher embryo fragmentation (p = 0.010).
Follicular fluid HDL micronutrients may play an important role in the development of the human oocyte as evident by embryo fragmentation during IVF.

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    • "Although the negative relation observed in our study between HDL-C and EFS was not statistically significant, we were able to show a significant positive relation between FR and HDL-C in FF. This may indicate the importance of HDL in fertilization which has also been reported by others (35). It has been shown that HDL and the proteins present in the structure of HDL could have a cytoprotective effects on oocyte and surrounding granulosa cells (36). "
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    • "Glutathione, as source of antioxidant is essential to oocyte health and play an important role in sperm decondensation post sperm penetration into oocyte (27, 32, 33). The negative effect of PUFA on reproductive outcomes may be accounted at two levels. "
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