Detection of Gene Expression in Embryonic Tissues and Stratified Epidermis by In Situ Hybridization

ArticleinMethods in molecular biology (Clifton, N.J.) 585:253-60 · January 2010with2 Reads
Impact Factor: 1.29 · DOI: 10.1007/978-1-60761-380-0_17 · Source: PubMed

    Abstract

    Although recent molecular biology advances provide a very effective way in determining localized gene expression, the visualization of the expression by in situ hybridization of whole-mount embryos or paraffin-embedded tissue sections continues to be an excellent method to determine overall gene expression. In this chapter, I review protocols designed to determine mRNA expression by in situ hybridization techniques, in particular, focusing on the developing stratified epidermis.