Optimization and validation of a high performance liquid chromatography method for rapid determination of sinafloxacin, a novel fluoroquinolone in rat plasma using a fused-core C-18-silica column
Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, No.325 Guohe Road, Shanghai 200433, PR China. Journal of pharmaceutical and biomedical analysis
(Impact Factor: 2.98).
10/2009; 51(4):889-93. DOI: 10.1016/j.jpba.2009.09.046
A novel, simple and rapid high performance liquid chromatographic method has been developed and validated for the determination of sinafloxacin, a new fluoroquinolone, in rat plasma using 96-well protein precipitation, fused-core C(18)-silica column (4.6mmx50mm, 2.7microm) packed with a new solid support, which is made of 2.7microm particles that consist of a 1.7microm solid core covered with a 0.5microm thick shell of porous silica.The chromatographic separation was achieved with a mobile phase of 20:80 (v/v) of acetonitrile and phosphate buffer (pH=3.0) at a flow rate of 1mlmin(-1). Fluorescence detection was employed with lambda(ex) 295nm and lambda(em) 505nm. Lomefloxacin was used as internal standard (IS). The total analysis time was as short as 3min. The method was sensitive with a limit of detection (LOD) of 2ngml(-1), with good linearity (R(2)=0.9996) over the linear range of 5-500ngml(-1). The intra-day and inter-day precision was less than 5.8% and accuracy ranged from 100.3% to 103.5% for quality control (QC) samples at three concentrations of 10, 50 and 400ngml(-1).The fused-core C(18)-silica column method offered high sample throughput, low injection volume and low consumption of organic solvents. The method was successfully employed in the pharmacokinetic study of sinafloxacin formulation product after tail vein injection to healthy rats.
Available from: Stacey Etheridge Degrasse
- "These advantages, compared to fully porous columns, enable the use of shorter columns and less solvent. Recent reports have demonstrated that the solid core packing material has a higher loading capacity, sample efficiency and resolution, as well as faster analysis times for the separation of small molecule drugs (Abrahim et al., 2010; Song et al., 2009; Wang et al., 2010; Zheng et al., 2009). Solid core technology has also recently been applied to a rapid HPLC method to detect the presence of wheat glutenin (Naeem and Sapirstein, 2007). "
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