In the contemporary digital landscape, the proliferation of cyber threats poses formidable challenges to organizations across various sectors. As adversaries become more sophisticated and the attack surface expands, traditional approaches to cybersecurity are proving insufficient in providing timely and effective threat detection and response. To address this critical gap, this research investigates the integration of Artificial Intelligence (AI) and Machine Learning (ML) techniques into real-time threat intelligence frameworks, aiming to enhance proactive threat detection and incident response capabilities. This study delves into the fundamental principles and methodologies underpinning AI and ML algorithms, exploring their applicability in the context of cybersecurity. By leveraging advanced algorithms such as neural networks, decision trees, and clustering techniques, AI-enabled systems can analyze vast volumes of heterogeneous data sources in real-time, extracting meaningful patterns and anomalies indicative of potential threats. Moreover, ML models can continuously learn from evolving datasets, refining their detection capabilities and adapting to emerging threats with minimal human intervention. Furthermore, the research investigates the integration of AI-driven analytics with existing security infrastructure, such as Security Information and Event Management (SIEM) systems and threat intelligence platforms. Through seamless integration and orchestration, AI-enhanced solutions can augment traditional security controls, providing contextualized insights and prioritized alerts to security analysts. Additionally, by automating routine tasks such as data correlation, anomaly detection, and threat hunting, these systems empower security teams to focus their efforts on mitigating high-priority threats and orchestrating timely incident response actions. The empirical evaluation of AI-driven threat intelligence solutions involves the development of prototype systems and the simulation of real-world cyber attack scenarios. By benchmarking the performance of AI algorithms against established metrics such as detection accuracy, false positive rate, and response time, this research aims to quantify the efficacy and scalability of AI-enabled threat detection mechanisms. Moreover, by conducting comparative analyses with traditional signature-based detection methods, insights into the added value of AI-driven approaches in enhancing overall cybersecurity posture are garnered. In conclusion, the integration of AI and ML technologies holds immense promise in revolutionizing real-time threat intelligence and incident response capabilities. By harnessing the power of advanced analytics and automation, organizations can proactively identify and mitigate cyber threats, thereby fortifying their defenses against evolving adversaries in the digital realm. However, challenges such as data privacy, algorithmic bias, and adversarial evasion tactics necessitate ongoing research and collaboration between academia, industry, and regulatory bodies to realize the full potential of AI-driven cybersecurity solutions.